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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... Probes were mixed with Human Cot-1 DNA (Invitrogen, 15279011) and salmon sperm (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 µg of human Cot-1 DNA (ThermoFisher Scientific) and then denatured in deionized formamide (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Immunology 2022Quote: ... anti-Human CD279(PD-1) antibody (Invitrogen, eBioscience, PE-Cyanine7), anti-Human CD223(LAG-3 ...
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bioRxiv - Immunology 2021Quote: ... 1:100 mouse anti-human CXCR5 PE-Cy7 (MU5UBEE, ThermoFisher) was added to each well at the start of stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (1:1000; AT180, Thermo Scientific, USA). The secondary antibodies used against above were anti-Mouse Cy3 (1:200) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (AT180; 1:1000, Thermo Scientific, USA), PHF-tauThr181 (AT270 ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 μg of recombinant human CDKL5 (1-498) (ThermoFisher, A33353) was combined with 3 μg of recombinant human SQSTM1/p62 protein (GeneTex ...
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bioRxiv - Immunology 2019Quote: ... ProcartaPlex Human Cytokine/Chemokine/Growth Factor Panel 1 (Thermo Scientific), as previously described [14] ...
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bioRxiv - Bioengineering 2019Quote: ... then with human-CD68 (FITC conjugated 1:20 dilution; Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-human GRZMK (Invitrogen, LS-C119554-50, 1:100), rabbit anti-human E-cadherin (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% antibiotic-antimycotic (ThermoFisher #1540062) ...
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bioRxiv - Microbiology 2022Quote: ... 1:5000 dilution rabbit anti-human Lcn2 (Invitrogen, Cat. 702248), 1:1000 dilution rabbit anti-human peptidylarginine deiminases 4 (PAD4 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat anti-human CD44 (1:500, ThermoFisher, 14-0441-82), Rabbit anti-human GFAP (1:400 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... goat anti-human Alexa Fluor 546 (1:400, Invitrogen Corporation) and goat anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rat anti-human CD44 (1:50, clone IM7, Invitrogen). The secondary antibodies included alexa fluor 488 labelled donkey anti-mouse (xxx ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-human IgG–Alexa594 (1:1000, ThermoFisher Scientific) for 30 min and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-C2-IQGAP1 and pcDNA3/RH-p122RhoGAP/DLC-1 plasmids were transfected into HEK293 cells using Lipofectamine 3000 (Invitrogen, MA).
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bioRxiv - Cancer Biology 2020Quote: ... pX330 plasmid harboring a p53-specific gRNA (Supplementary Table 1) was transfected into HEK293 and HCT116 cells using Lipofectamine 2000 (Invitrogen). Two days later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 stably expressing the tetracycline (tet)-repressor (Flp-In T-REx HEK293, Invitrogen, Carlsbad, CA, USA) were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells: HEK293 cells (CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045) were cultured in DMEM high glucose (Gibco) and supplemented with 10% FCS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-lysosomal-associated membrane protein-1 (LAMP-1, 1:200 #14-1079-80; Thermo Fisher Scientific), followed by incubation with secondary antibodies (anti-rabbit conjugated with Alexa Flour 488 ...
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bioRxiv - Bioengineering 2019Quote: MDA-MB-231 human breast cancer cells were cultured using 1:1 DMEM:F12 medium (Life Technologies) supplemented with 50 µg/ml gentamicin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Huh7 human hepatocellular carcinoma cells were grown in DMEM/F-12 1:1 medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Hec1 (1:1000, human monoclonal) and Anti-phospho-Paxillin (1:100, rabbit polyclonal, pTyr31, Invitrogen). Secondary antibodies diluted in antibody dilution buffer was added along with the conjugated Phalloidin-TRITC (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were stained with mouse-anti-human-ZO-1 1:500 (Thermo Fisher Scientific, 33-9100) or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was pre-cleared with 1:1 protein A:protein G Dynabeads (Life Technologies) and immunoprecipitation was carried out with H3K27ac-bead complexes at 4°C overnight ...
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bioRxiv - Genetics 2020Quote: ... 50 uL of a 1:1 mix of Protein A (Cat #10008D, Invitrogen) and Protein G Dynabeads magnetic beads (Cat #10004D ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant full-length human G2019S-LRRK2 protein (Invitrogen) was used as a protein standard ...
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bioRxiv - Bioengineering 2022Quote: ... Human BMP-4 Recombinant Protein (Gibco, Catalog # PHC9534). For cell culture of iPSCs on niches ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Vitronectin (recombinant human protein) was from Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen, PHG0313), 1.1 μM hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...