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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in DMEM with 10% fetal bovine serum (FBS) and 1:100 penicillin-streptomycin (ThermoFisher). In 24-well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... and renilla luciferase plasmid (1 ng) were transfected into HEK293 cells or HCT116 cells by lipofectamine2000 (Invitrogen, 11668030) following manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells were transiently transfected with 1 µg of each cDNA using Lipofectamine 2000 (Invitrogen, Carlsbad CA, USA). After 12 h of transfection ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
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bioRxiv - Bioengineering 2023Quote: ... and rabbit polyclonal cementum protein 1 (1:100, Thermo Fisher Scientific) prepared in a solution of 0.3% Triton X-100 ...
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bioRxiv - Plant Biology 2024Quote: ... Mixture of Dynabeads with proteins A and G (1:1) (Invitrogen) was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research) ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Neuroscience 2023Quote: ... and 1.3 nM human insulin-like growth factor 1 (hIGF-1, Thermo Fisher Scientific). The growth media were changed after 24 h and further regularly changed every 4 days ...
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bioRxiv - Immunology 2019Quote: ... Protein A and Protein G coupled magnetic beads (mixture 1:1, Dynabeads, Thermo Fisher, 10002D and 10004D) were pretreated with BSA (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
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bioRxiv - Bioengineering 2022Quote: ... A 40 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for an additional 4 h at 4°C on a rotator (400 rpm) ...
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bioRxiv - Immunology 2023Quote: ... A 20 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4 h at 4°C with rotation (50 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length human LRRC4B was subcloned and inserted into the pcDNA6-V5-His vector (Invitrogen). FAM19A5 (NM_001252310.1 ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclear factor erythroid 2-related factor 2 (NRF2) (ThermoFisher Scientific, Carlsbad, CA), samples diluted 1:2 ...
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bioRxiv - Neuroscience 2023Quote: ... and the related SilencerTM select siRNA negative control (Thermo Fisher Scientific, 4390843) were purchased ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled LDL (1 mg) from human plasma (Thermo Scientific) was incubated with 50 nmol cholesterol (Top-Fluor cholesterol ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-human CD133 (Thermo Fisher Scientific, 1:1000) or rabbit anti-human Vimentin (Proteintech Group ...
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bioRxiv - Cancer Biology 2019Quote: ... Rabbit anti-human-Ki67 (1:20, Thermo Fisher Scientific), Rabbit anti-Chromogranin A (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 1% human melanocyte growth supplement (HMGS, Gibco) and 1% Pen-strep ...
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bioRxiv - Neuroscience 2021Quote: ... human tau (HT7; 1:1000; MN1000; Invitrogen, Waltham, MA), poly-tau (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... anti-human BCL-XL (clone 7B2.5, Invitrogen, 1:50), and p24 antibodies (anti-HIV core antigen ...
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bioRxiv - Immunology 2021Quote: ... then 1:5000 Goat anti-Human (ThermoFisher Scientific A18817) was added ...
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bioRxiv - Immunology 2021Quote: ... then 1:5000 Goat anti-Human (ThermoFisher Scientific A18817) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-human CD31 (1:500, Invitrogen, 14-0319-82), anti-PyMT (1:200 ...
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bioRxiv - Immunology 2021Quote: ... anti-human CD8 (Invitrogen, 53-0008-82, 1:100), rabbit anti-human GRZMK (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Human recombinant insulin (10 μg ml−1, Gibco #12585014) was additionally included in the culture media for MCF7 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-human CD68 (Thermo Fisher, MA5-13324, 1:100), anti-human Chi3l1 (Proteintech ...
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bioRxiv - Bioengineering 2023Quote: ... rabbit anti-human IgG antibody (Invitrogen, 31423, 1:20,000) and HRP-conjugated anti-M13 antibody (Sino Biological ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-human Cx36 (Invitrogen, 36-4600, 1:500) and chicken anti-GFP (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... 1 ng/mL human FGF2 (ThermoFisher #68-8785-63), 1% antibiotic-antimycotic (ThermoFisher #1540062) ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-human AlexaFluor 647 (Invitrogen, A21445, 1:1000). For Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg/mL recombinant human insulin (Thermo Fisher Scientific), 0.55 µg/mL human transferrin (Thermo Fisher Scientific) ...