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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
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bioRxiv - Genetics 2023Quote: ... CMs were loaded with 1 µM Fluo-4 AM (Life Technologies, Carlsbad, CA) in HBSS solution for 30 minutes at 37°C and washed ...
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bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight at 4°C and with secondary antibodies (Alexa fluor, Invitrogen 1:800) during 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... at a 4:1 PEI:DNA mass ratio and hybridoma SFM (Cat# 12045076, Thermofisher). An empty vector pcDNA3.1+ (Cat# V79020 ...
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bioRxiv - Cell Biology 2024Quote: ... to which 1 μl of 4′,6-diamidino-2-phenylindole (DAPI) (D1306, Invitrogen) was added ...
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bioRxiv - Neuroscience 2024Quote: ... and processed for 24 hrs at 4 degrees with NeuroTrace (1:500, Invitrogen) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C with AlexaFluor-568 streptavidin (1:2000, Invitrogen) and anti-somatostatin antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1mM Probenecid and 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco). Cells were imaged at Ex/Em = 490/525nm for Cal-520 AM fluorescence intensity for a total of 120 seconds ...
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bioRxiv - Bioengineering 2024Quote: ... followed by primary antibodies (IL-4 Abcam ab9811, 1:100; IL-13 Invitrogen PA5-102574 ...
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bioRxiv - Biophysics 2024Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 4× SSC and 1:100 dilution of SUPERase In RNase inhibitor(AM2694; Invitrogen). The 100 μl of serial hybridization buffers for 70 rounds of seqFISH+ imaging (including smFISH genes ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Ran (1:500; O/N at 4°C; MA511198; Thermo Fisher), mouse anti-RCC1 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific; 1 h, 22 C). The ligation mix was purified (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... Cells were passaged 1:2-1:6 every 2-4 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 µl/cm2 ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuolar membranes were stained with 1 μL of 1 mg/mL solution of FM 4-64 (T3166; Invitrogen) in DMSO added at the beginning of incubation of cells in YPD medium ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 μL Tris pH 6.5 1 M and 1 μL Proteinase K (20 mg/ mL, Thermo Fisher Scientific) and incubated shaking for 3 hours at 45°C ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were incubated at 4°C overnight (Abcam, ab138501, 1:1,000; Thermo Fisher Scientific, AM4302, 1:5,000). Horseradish peroxidase-conjugated secondary antibodies were incubated for 1 hr at room temperature (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Immunology 2022Quote: ... mBECs treated with IFNγ were fixed with 4% paraformaldehyde in PBS for 15 minutes and stained with antibodies against Zona occludens-1 (ZO-1, 1:250 Invitrogen 33-9100) and CXCL10 (1:500 Abcam ab9938) ...
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bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in the NGS for at least 4 hrs and incubated overnight at 4°C with secondary antibodies: goat anti-rabbit Alexa Fluor 488 (Invitrogen Cat# A11070; 1/400) and goat anti-mouse HRP-conjugated (Thermo Fisher Scientific Cat# G-21040 ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Cancer Biology 2020Quote: ... new organoids were fixed with 4% paraformaldehyde for 1 day and then incubated with the phalloidin (1:40; Invitrogen) and Hoechst 33342 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Primary staining’s were performed overnight at 4 °C with antibodies to TRF2 and POT-1 (1:300; Abcam and ThermoFisher,); CD3 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with rocking for 1 h at 4 °C with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) pre-equilibrated in SEC buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... 2011)) at 1:400 concentration at 4°C and secondary antibodies Alexa Fluor 647 (1:500; Thermo Fisher Scientific) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 4 μM Mag-Fluo-4 (ThermoFisher Scientific) and 1 μM F-127 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Purified total RNA (1-4 μg) was treated with TURBO DNA-free (ThermoFisher, AM1907) and reverse transcribed with Superscript IV (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the fluorescent dye 4’,6-diamidino-2-phenylindole (DAPI; 1:1,000, Molecular Probes), respectively.
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bioRxiv - Developmental Biology 2020Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI: nuclear counterstain; 1:1000; ThermoFisher, Waltham, MA).
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bioRxiv - Microbiology 2022Quote: ... mixed 4:1 with reducing NuPAGE™ LDS sample buffer 4x (ThermoFisher Scientific, NP0007) and boiled for 10 minutes ...