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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were washed with PAT for 5 hours and then incubated for 3 days in PAT-NGS with goat anti-rabbit AlexaFluor-488 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... Eluted vRNA was subsequently used as a template for cDNA synthesis and reverse-transcribed using the reverse primer SHIV.Env.R1 (5’-TACCCCTACCAAGTCATCA-3’) and SuperScript III reverse transcriptase (Thermo Fisher Scientific #18080044). cDNA was serially diluted to identify the dilution at which <30% of wells contained PCR amplicons of the correct size ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from individual tissues or pools of 3–5 midguts using TRIzol reagent (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... at 30°C to an optical density at 600 nm of 3–5 and were harvested by centrifugation at 4000 rpm (Sorvall GS3 or SLA-3000, Thermo Scientific) for 8 min ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were washed 3 times for 5 min with PBTx before incubation at room temperature in 1 μg/mL DAPI (Invitrogen, ThermoFisher Scientific) solution in PBS for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Both the nuclear and chromatin pellet fractions were sonicated 3 times (5 seconds on, 20 seconds off; 30% amplitude; Sonic Dismembranator Model 500 (Fisher Scientific)) on ice ...
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bioRxiv - Immunology 2023Quote: ... followed by first strand cDNA synthesis using either a NSP4 gene-specific forward primer with a random nucleotide tag sequence (5’-cggtcatggtggcgaataaGCGGCCTTTAATGTGGAATG-3) or without any primer according to the SuperScriptTM III Reverse Transcriptase protocol (Invitrogen, 18080044). cDNA synthesis was then completed followed by a PCR using an eGFP gene-specific reverse primer (5’-CACCTTGATGCCGTTCTTCT-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed 3 times for 5 min with PBS and incubated with Image-iT FX Signal Enhancer (Thermo Fisher Scientific) for 30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... slides or coverslips were washed 3 times for 5 min in PBS and incubated with appropriate species-specific Alexa Fluor-conjugated secondary antibodies (Molecular Probes). DNA was counterstained with Hoechst 33342 (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... KHDRBS3 knockdown was performed by transfection of 50 nM of non-targeting siRNA (si-CTL) or specific siRNA targeting KHDRBS3 (si-KHDRBS3: 5′ GCGAAGUACUUCCAUCUCAAUGA-3’) (Dharmacon) with Lipofectamine RNAiMAX Transfection Reagent (Invitrogen, #13778075) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse calvariae were dissected from 1–3 days old neonatal mice and digested sequentially 5 times for 25 minutes in α-MEM (Gibco) containing 0.1% collagenase (Roche ...
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bioRxiv - Neuroscience 2024Quote: Mouse bone marrow-derived macrophages (BMDMs) were collected from the femur and tibia from 3- to 5-month-old mice and grown in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed with PBS (3 times x 5 minutes) and mounted using Fluoromount-G (Thermo Fisher Scientific, #00-4958-02).
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 3-5 minutes at 37°C with a 1:10 dilution of trypsin 2.5% (Thermo Fisher #15090-046) in PBS (HSCs ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed extensively (3 x 5 mins) in TBS-tween20 and incubated in appropriately labeled secondary antibodies (Invitrogen Carlsbad, CA) for 1 h at RT ...
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bioRxiv - Genomics 2024Quote: ... Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’) and MultiScribe Reverse Transcriptase (Invitrogen, 4311235). Following cDNA synthesis ...
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bioRxiv - Cell Biology 2020Quote: ... The following primers were used for TaqMan® probe-based assay (Applied Biosystems) was used for qPCR of DUOX1 (Hs00213694_m1) ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were excluded based on propidium iodide staining (Molecular Probes, Life Technologies). For analysis of cytokine production ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were excluded based on propidium iodide staining (Molecular Probes, Life Technologies). For analysis of cytokine production ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected tissue chunks were transferred to cold HBSS-based dissociation media (Thermo Fisher Scientific Cat ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected tissue chunks were transferred to cold HBSS-based dissociation media (Thermo Fisher Scientific Cat ...
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bioRxiv - Microbiology 2019Quote: ... and introduced into the plasmid with restriction enzyme-based techniques (Thermo Scientific, Finland). The ligated plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2019Quote: Plasmids producing the Sup35NMG protein were based on either pYES2 (Thermo Fisher Scientific) or pCM190 (46 ...
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bioRxiv - Cell Biology 2019Quote: ... Taqman®-based assays were performed according to the manufacturer’s instructions (Applied Biosystems). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted using a TRIzol-based method (Life Technologies, CA, USA). RNA quality control was performed via several steps ...
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bioRxiv - Immunology 2021Quote: ... Immunoblot images were processed using web-based iBright Image Analysis software (Thermo Fisher). Flow cytometry data was analysed using FlowJo (FlowJo ...
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bioRxiv - Microbiology 2020Quote: ... or flow cytometry-based analysis of infected blood stained with Hoescht 33342 (Invitrogen). Blood from infected mice was collected by cardiac puncture under terminal anaesthesia ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium was based on Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific, 11960044), supplemented with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... the gels were stained using ProQ diamond stain based on manufacturer’s protocol (Invitrogen) to detect phosphoproteins ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability was quantified using the fluorescence based Alamar Blue assay (Invitrogen, Cat# DAL1025). Alamar blue was added to each well in 10 μl aliquots ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using a SYBR Green-based system (Thermo Fisher Scientific), and data were calculated using the 2-ΔΔCT method ...
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bioRxiv - Molecular Biology 2019Quote: ... We assessed miRNA expression by TaqMan-based qPCR (Thermo Fisher, Part Number 4427975). We reverse transcribed miRNAs with a TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher ...
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bioRxiv - Physiology 2020Quote: ... The NALCN construct was made in a vector based on pTracer-CMV2 (Invitrogen) and modified to also express eGFP under a separate promoter ...
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bioRxiv - Bioengineering 2019Quote: ... a live/dead-assay based on fluorescein diacetate (FDA; F1303, Thermo Fisher Scientific) and propidium iodide (PI ...
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bioRxiv - Systems Biology 2021Quote: ... The serum-based medium (denoted serum+LIF) consisted of high-glucose DMEM (Gibco) supplemented with 15% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... linearized and subjected to T7-based in vitro RNA transcription (Thermo Fisher Scientific). Capped viral RNA was electroporated into baby hamster kidney cells and supernatant was subsequently transferred to Vero E6 cells one day after electroporation for stock production ...
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bioRxiv - Microbiology 2022Quote: BMDMs were washed and harvested using HBSS-based cell dissociation buffer (Thermo scientific) by incubating at 37°C for 10 min and pipetting with ice-cold HBSS ...
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bioRxiv - Pathology 2021Quote: ... incubated (without detergent-based permeabilization) with AlexaFluor 594 Phalloidin (Invitrogen; 20 min, RT) to visualize actin cytoskeleton ...
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bioRxiv - Plant Biology 2020Quote: ... was used for TaqMan chemistry based assays and Power SYBR mastermix (Applied Biosystems) for SYBR Green chemistry based assays ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were excluded based on propidium iodide staining (Molecular Probes, Life Technologies). For analysis of cytokine production ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were excluded based on propidium iodide staining (Molecular Probes, Life Technologies). For analysis of cytokine production ...