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Citations for Thermo Fisher :
3301 - 3350 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... then subjected to a 1 h incubation with secondary antibody (Goat anti-Rabbit IgG, 1:200, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 546 (RRID: AB_2534071, A-11003, ThermoFisher), goat anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2024Quote: ... three replicas of each cell line and condition were incubated for 24 h with 0.15 µg/mL of Colcemid (Gibco Thermofisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... embryos were then incubated with Goat anti-Mouse IgG (H+L) Alexa Fluor 488 (1:150, Cat# A28175, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... followed by cross-absorbed goat anti-rabbit IgG (H\&\L) conjugated to either AlexaFluor 488 or 594 fluorophores (Invitrogen).
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bioRxiv - Physiology 2024Quote: ... total RNA was reverse transcribed into cDNA using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1682) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... Alexa-Fluor 568 Donkey anti-Goat IgG (H+L) Cross-adsorbed Secondary Antibody (Thermo-Fisher Scientific, A11057, 1:500). Images of fixed cells were acquired with a Zeiss LSM 700 confocal laser scanning microscopy (Biology Image Center ...
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bioRxiv - Microbiology 2024Quote: ... and 100 µl per well of secondary antibodies [goat anti-hamster IgG(H+L)-conjugated with HRP (Thermo Fisher, cat# PA1-29626 ...
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bioRxiv - Microbiology 2024Quote: ... was used and RNA samples were converted to cDNA with RevertAid H Minus Reverse Transcriptase kit (Thermo Fisher Scientific) and analysed as previously described (Lemberg et al. ...
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bioRxiv - Molecular Biology 2024Quote: shRNA transfected cells were treated with 2 mM HU for 4 h along with 0.1 µg/mL colcemid (KaryoMAX, Gibco) 30 h post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... antibody binding was detected with the corresponding fluorescent secondary antibodies in incubation buffer for 1 h at RT (1:1,000, Invitrogen) and the sections were fixed with 4 % PFA for 10 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... antibody binding was detected with the corresponding fluorescent secondary antibody in incubation buffer for 1 h at RT (1:1,000, Invitrogen). The sections were rinsed again with PBST and PBS ...
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bioRxiv - Microbiology 2024Quote: ... and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody were purchased from ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher). Proteins were concentrated to 50 μM in pull-down buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h at room temperature followed by a secondary antibody Anti-Mouse Alexa 555(A-21424, Thermo Fisher) or Anti-Mouse Alexa 647 (A-21235 ...
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bioRxiv - Pathology 2024Quote: ... incubated with the host-specific antibodies conjugated with Alexa Fluor fluorophores (dilution 1:1000; IgG H+L, Life Technologies) and DAPI (1 µg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... followed by DNase digestion and first-strand cDNA synthesis (Maxima H Minus Kit, random hexamer primers, Thermo Scientific K1652). RT-qPCRs were performed in technical triplicates with primers binding to the 3’ UTR of ndhm1 (Table S6) ...
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bioRxiv - Microbiology 2024Quote: ... samples were incubated at 65°C for 1 h followed by the addition of 3.2U proteinase K (Thermo Fisher) and incubation at 60°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... After 72 h BMDM were fixed with ice-cold methanol for 3 minutes and washed twice with PBS (Gibco); nuclei were then stained with DAPI (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total RNA was converted to cDNA using the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher, K1652) following the manufacturer’s “RT-qPCR-First Strand cDNA Synthesis” protocol using random hexamers and oligo (dT)18 primers with an extended 50°C synthesis step for 30 min ...
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bioRxiv - Immunology 2024Quote: ... stably expressing the mouse class I allele H-2Kb (44) were maintained in Dulbecco’s modified Eagle medium (DMEM, ThermoFisher) with 10% FBS supplemented with 0.5 mg/ml G418 (Hyclone) ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated for a total of 4h with the last 3.5 h in the presence of protein transport inhibitors (Invitrogen). Cells were then harvested ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at room temperature in a PBS solution containing 0.2 µg/mL Hoechst 33342 (H3570, ThermoFisher) and 1 µg/mL Streptavidin-Alexa488 (016-540-084 ...
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bioRxiv - Cell Biology 2024Quote: Liver tissue sections were processed for H&E and images were taken using Evos XL Core (Thermo Fisher Scientific). Liver tissue sections were first deparafinized by heating at 85°C for 10 min followed by treatment with decreasing gradients of alcohol ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 x 10 minutes in PBS and incubated for 2 hours at RT with the following secondary antibodies (1:500): goat anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 546 (RRID: AB_2534071, A-11003, ThermoFisher), goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... and pieces incubated in secondary antibodies [anti-rat secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rabbit 568 secondary antibody (H+L ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was prepared using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). Quantitative PCR was performed using fast SYBR® Green PCR master mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... GST and GST-NbSTIR1 were induced with 0.4 mM IPTG for 4 h at 37℃ and purified using Glutathione agarose resin (ThermoFisher). NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher) ...
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bioRxiv - Pathology 2024Quote: ... 0.5mL of the secondary antibody Alexa Fluor TM 488 conjugated donkey anti-goat IgG (H+L) (Cat# A11055, Invitrogen) diluted 1:500 in PBS was then added and incubated for 90 minutes at room temperature while rocking ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μM of monomeric Aβ was incubated for 24 h at 4°C in Ham’s F12 media (Gibco, #11765054) to make a 50 μM stock of oligomeric Aβ as published previously (14,15) ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa 647-conjugated goat anti-rabbit IgG (H+L) antibody (no. A-21245; Thermo Fisher Scientific; 1:100 dilution); Alexa 546-conjugated goat anti-rat IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa 546-conjugated goat anti-rat IgG (H+L) antibody (no. A-11081; Thermo Fisher Scientific; 1:500 dilution).
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bioRxiv - Immunology 2024Quote: ... The transfected cells were harvested 48 h post-transfection with RIPA buffer and cocktail protease inhibitors (Thermo scientific, # A32965) for 30 min on ice then scraped off the dish ...
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bioRxiv - Immunology 2024Quote: ... at 37°C for 1 h then washed 3x with 0.5% Tween 20 in PBS (Catalogue# 18912014, Thermofisher Scientific). Slides were mounted with ProLong anti-fade mounting medium (Catalog # P36934 ...
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bioRxiv - Immunology 2024Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
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bioRxiv - Synthetic Biology 2024Quote: ... and incubated for 1 h at room temperature with 6x-His tag monoclonal antibody (HIS.H8) (Cat#MA1-21315, ThermoFisher). After three more PBST washes ...
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bioRxiv - Immunology 2024Quote: ... the cells were detected with goat anti-human IgG [H+L] cross-adsorbed secondary antibody Alexa Fluor 647 (Invitrogen). propidium iodide (0.75 µM ...
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bioRxiv - Genomics 2024Quote: ... Cells were plated in a 6-well plate and transfected 24 h after plating in DMEM + 0.1% foetal bovine serum and 1% penicillin-streptomycin using Lipofectamine 2000 (Invitrogen). 800 ng of dual luciferase reporter containing CREB3 response element was co-transfected with 2400 ng of pCMV6 ...
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bioRxiv - Biochemistry 2024Quote: ... Cell debris was removed by centrifugation 17.000 rpm at 4°C for 1 h (A27 rotor; Thermo Scientific™). The supernatant was loaded on a 5-ml His Trap HP Ni-NTA column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated 0.75-1 h with the conjugated secondary antibodies (alexa fluor-488, 594 or 647, Life Technologies), followed by washes in PBS and counterstaining with DAPI ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with rocking for 1 h at 4 °C with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) pre-equilibrated in SEC buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were rinsed four times with PBS with 0.01% Triton X-100 and incubated for 1 h with 1:500 fluorescent-conjugated secondary antibodies (ThermoFisher) and 2.8 µM DAPI diluted in 1% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads; Invitrogen) were washed on a magnetic stand (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were then incubated for 1 h at room temperature with two primary antibodies: mouse anti-6X-HisTaq (dilution 1:1.000, MA1-21315, Invitrogen) and mouse anti-VSV-M (dilution 1:1.000 ...
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bioRxiv - Bioengineering 2024Quote: ... falciparum-infected macrophage was isolated at appropriate time point (24 h) using the TRIZOL reagent (Invitrogen, Grand Island, NY) and quantified using a Nanodrop ND-1000 spectrophotometer (Thermo Fischer ...
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bioRxiv - Cell Biology 2024Quote: ... AB_2533967) and Goat anti-Mouse IgG (H + L) Secondary Antibody (Cat# 31430, RRID: AB_228307) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... RNAs were labeled at room temperature for 4 h in the dark by adding 5’-(iodoacetamido)fluorescein (IAF) (ThermoFisher) dissolved in DMSO to a final concentration of 7.5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (H+L) Cross-adsorbed secondary antibody with Alexa Fluor 555 (Invitrogen, cat no. A-21428), and DAPI (Invitrogen ...