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Citations for Thermo Fisher :
3201 - 3250 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Cells were rested at 37 ºC for 2 h in RPMI Complete Media (RPMI 1640 medium (Life Technologies, A1049101) with 10% fetal bovine serum (Life Technologies ...
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bioRxiv - Immunology 2024Quote: RNA was purified from various T cell subsets cultured under Th0 conditions for 12 h using Trizol reagent (Invitrogen). cDNA was synthesized using reverse transcription kit (Progema) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with 1 µM Rhonox-M for 1 h or lysotracker deep red (Thermo Fisher Scientific L12492) according to the manufacturer’s instructions for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was centrifuged (158,000 g, 4 °C, 1 h) and the cleared lysate incubated with Ni-NTA agarose (Invitrogen) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: First-strand cDNA synthesis was performed using RevertAid H Minus Reverse Transcriptase (200 U/µl; EP0451; Thermo Fisher Scientific) following the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2023Quote: ... while a secondary antibody comprising goat anti-mouse IgG (H&L) conjugated with alkaline phosphatase (ThermoFisher Scientific, MA, USA) was employed ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA obtained (2μg) was then reverse transcribed to cDNA in duplicate using the SuperScript III RNase H-Reverse Transcriptase kit (Invitrogen) with random primers (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from equal volumes of immunoprecipitated and input RNA using Maxima H Minus reverse transcriptase (Thermo Fisher) and random hexamer primers ...
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bioRxiv - Systems Biology 2023Quote: ... Gels were run at 200 V for approximately 1 h and incubated with SYBR Gold nucleic acid stain (Invitrogen), diluted 1:10,000 in TBE buffer (90 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 1 μg total RNA was carried out using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific) and oligo dT12–18 (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... MPSF and NPSF were treated for 1 h with IM 0.5 μM or DMSO as vehicle in DMEM high glucose (#41965-039; Gibco). Mouse and NMR SV40-Ras skin fibroblasts were treated for 15 and 10 min with IM 2.5 μM ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor™ 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (1:250, Invitrogen, A31573). Alexa Fluor 647 Phalloidin (1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... About 24 h post-transfection cells were lysed in 500 μl of Pierce IP lysis buffer (Thermo Fisher Scientific) supplemented with Halt protease inhibitors and Benzonase nuclease (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM ...
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bioRxiv - Immunology 2023Quote: ... then incubated with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody conjugated to HRP (ThermoFisher, #G-21040) at a 1:350 dilution in 1X PBS with 0.05% Tween® 20 and 1% fetal bovine serum overnight at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Signal was enhanced using a triple western blot protocol with biotin conjugated goat antirabbit IgG and Alexa Fluor 680 conjugated S-32358-streptavidin (1:100,000)35 and 31820-Goat anti-rabbit IgG (H+L) secondary antibody Biotin IgG (1:100,000) from Thermo Fisher Scientific were used.
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated with Alexa Fluor 555-coniugated goat anti-mouse IgG (H+L; Thermo Fisher Scientific, 1:400) and Alexa Fluor 488-coniugated goat anti-rabbit (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Plant Biology 2023Quote: ... One µg of total RNA was reverse transcribed for each sample with RevertAid H Minus Reverse Transcriptase (Thermo Scientific). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), and subsequently washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer) and washed four times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated for 2 h at RT and the proteins were detected with Supersignal West Pico (34580, Thermo Scientific) and imaged by using iBright 1500 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and BN-PAGE gels were run for 2-2.5 h at 150 V using NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Following preset incubation times (0, 0.5, 1, 3, or 5 h) cells were harvested by trypsinization (500 μl TrypLE, Gibco) for 5 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... orca-pro-(h)IL-1α and pro-IL-1β genes were synthesized and cloned to pcDNA3.1(+) vectors (Life Technologies). pro-α-mat-β chimeric expressed the Met1to Arg112 of human IL-1α ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used were as follows: for neurons: Goat anti-Rabbit IgG (H+L) AF514 (Invitrogen, A-31558); Goat anti-Mouse IgG AF594 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM ...
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bioRxiv - Bioengineering 2023Quote: ... was used as a primary antibody and HRP conjugated Goat anti-rabbit (H+L) IgG (Thermo Fisher Scientific, USA) was used as a secondary antibody for detecting target proteins ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Tachyzoites or parasites exposed to 48 or 96 h differentiation stress as explained before were conserved in TriZol (Invitrogen) solution at −80°C until use ...
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bioRxiv - Biochemistry 2022Quote: ... After transfection with Lipofectamine 3000 and the indicated plasmids or siRNAs for 48 h according to the manufacturer’s instructions (Invitrogen), the transfection efficiency was assessed by Western blotting (WB ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were incubated with RNA-aptamer-coupled beads for 3.5 h at 4°C under rotation in the presence of 60U RNasin (Ambion). For each pull-down ...
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bioRxiv - Cancer Biology 2022Quote: ... fetal liver cells were thawed and cultured for 48 h in alpha-minimum essential media (alpha-MEM) Glutamax (Gibco) containing 10% FCS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 µg/ml of polybrene) for 24 h and selected with 0.5 mg/ml of Geneticin/G418 Sulfate (Gibco) for at least one week ...
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bioRxiv - Cancer Biology 2023Quote: ... Corning® Bio-Coat® Matrigel® Invasion Chambers with 8.0 μm PET membranes were rehydrated for 2 h at 37 °C using NB-A medium with only 100 U/ml Penicillin 100µg/mL Streptomycin (Gibco) added ...
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bioRxiv - Developmental Biology 2022Quote: The ability of the tissues to produce testosterone was assessed after 3 h of incubation with DMEM/F12 (Gibco) containing 0.1% BSA ...
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bioRxiv - Immunology 2023Quote: ... washed with PBS and cultured for 48 h in 1ml of complete Roswell Park Memorial Institute (RPMI) 1640 (Gibco) containing 2 mM L-glutamine (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... for 2–5 h at 37°C followed by a 5 min incubation in TrypLE express (Thermo Fisher Scientific) to generate small clumps of corneal endothelial cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and after 16-h cells were left untreated or treated for 30 min with MitoTracker™ Orange CMTMRos (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with donkey anti-murine IgG-(H+L)-AF488 (20 µg/mL, #A21202, Invitrogen) on ice for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... was pre-soaked in buffer H and then overlaid on a positively-charged nylon membrane (BrightStar™-Plus, Invitrogen). The protein-mRNA mixtures were applied onto a 96-well slot-blot apparatus and then filtered through the double membranes by vacuum ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated at 37 °C for 1 h in buffer H1 (50% formamide (FA, Cat. # AM9342, ThermoFisher), 10% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... organoids were passaged using H+++B Medium (Advanced DMEM/F-12 [Thermo Fisher], HEPES pH7.2-7.5 [10mM, Thermo Fisher] ...
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bioRxiv - Immunology 2023Quote: ... or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L) antibody (1:500; Invitrogen, Cat. PA1-28597) in blocking buffer for 1-1.5 hours at RT ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was then performed on the RNA template using Maxima H-Reverse Transcriptase (Thermo Fisher, cat. no. EP0753) to create a DNA-RNA hybrid ...
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bioRxiv - Immunology 2023Quote: ... For qRT-PCR cDNA was synthesized using Maxima H Minus cDNA synthesis kit (catalog no. M1681; Thermo Fisher Scientific). PrimePCRTM PCR Primers assay plates were custom made with validated primers for use with EvaGreen dye-based chemistry (Bio-RAD ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were continued for another 3.5 h prior to addition of 1 µM NAO and/or 1 µM ReAsH-EDT2 reagent (Invitrogen). Cultures were incubated for 1 h at room temperature and washed with 1X BAL wash buffer per manufacturer kit instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The yeast two hybrid cDNA library was constructed from the mRNA of seedlings exposed to -1.2 MPa stress for 96 h according to the CloneMinerTM II cDNA library construction kit (Invitrogen) and then cloned into prey vector pDEST22 ...