Labshake search
Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... R: 5′- AAAGCACCGACTCGGTGCCAC-3′) and used as a template for in vitro transcription using MEGAshortscript T7 kit (Ambion/Thermo Fisher Scientific AM1354). 2ng gRNA was injected together with 1.9nM Cas9 protein (New England Biolabs M0386T ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized using 5 µg RNA as a template per reaction with the Superscript III First Strand Synthesis System Kit (Invitrogen, 18080-051) and its provided random hexamers ...
-
bioRxiv - Genomics 2022Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) following the manufacturer protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... The library is quantified through qPCR (Invitrogen™ Collibri™ Library Quantification Kit; QuantStudio™ 5 Real Time PCR Systems, APPLIED BIOSYSTEMS).
-
bioRxiv - Plant Biology 2024Quote: RNA ligase-mediated rapid amplification of cDNA ends (5’-RLM-RACE) was performed using FirstChoice® RLM-RACE kit (Thermo Fisher Scientific) following the manufacturer protocol and omitting the dephosphorylation and decapping steps ...
-
bioRxiv - Biochemistry 2024Quote: ... Complementary DNA (cDNA) was synthesized from 5 µg of the total RNA using the SuperScript IV Reverse Transcriptase Kit (Thermo Fisher Scientific) following the manufacturer’s recommendations.
-
bioRxiv - Immunology 2024Quote: ... qPCR was performed on an Applied Biosystems Quantstudio 5 machine using the AgPath-ID One-Step RT-PCR kit (Applied Biosystems, 4387391) and Taqman primers as per manufacturer recommendations ...
-
bioRxiv - Physiology 2024Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) following the manufacturer protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Phase separation in cells lysed in TRIzol was induced with 50% isopropanol and 0.5% 2-mercaptoethanol and RNA was extracted from the aqueous phase using the MagMAX mirVana Total RNA Isolation Kit (Thermo Fisher, A27828) on a KingFisher Flex Magnetic Particle Processor according to the manufacturer’s protocol with 1-2 million cells input ...
-
bioRxiv - Cancer Biology 2021Quote: ... 4mM L-Glutamine and 1X ITS (5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium) (all from Gibco, Thermo Fisher Scientific, Waltham, MA). The Wit49 cell line was a gift from Dr ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 days post-transfection cells were selected with 5 μg/mL blasticidin (Thermo Fisher Scientific). When the survival rate of the cells improved ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 μl 10x Turbo DNase buffer and 5 μl 2U/μl TurboDNase (Thermo Fisher Scientific) were added to 85 μl of sample and incubated for 20-30 min at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... ×5 mm C18 PepMap 100 trap column with 5 μm particle size (Thermo Fisher Scientific) to desalt and concentrate the samples before loading onto the C18 nanocolumn for separation ...
-
bioRxiv - Biophysics 2021Quote: ... stock was prepared at 5 mg/mL in dimethyl sulfoxide (#67-68-5, Fisher Scientific) and stored at −20 °C ...
-
bioRxiv - Microbiology 2019Quote: ... 1x 10^5 cells were resuspended in water containing 5 µM propidium iodide (ThermoFisher Scientific) and incubated at room temperature for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
-
bioRxiv - Genomics 2019Quote: ... Selected cells were frozen at 5 × 106 cells/mL in 5% DMSO (Thermo Fisher Scientific) and aliquots were used in the genomic MPRA.
-
bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
-
bioRxiv - Biochemistry 2019Quote: ... ×5 mm C18 PepMap 100 trap column with 5 μm particle size (Thermo Fisher Scientific) to desalt and concentrate the samples before loading onto the C18 nanocolumn for separation ...
-
bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) human serum (PAA Laboratories GmbH) and 5 % v/v Albumax II (Invitrogen) at 37°C in a gas environment of 5 % CO2 ...
-
bioRxiv - Immunology 2023Quote: ... and STX-S_pLenti at a ratio of 5:5:1 using Lipofectamine 3000 (ThermoFisher, L3000008) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: ... and a C18 PepMap 100 trap column (5 mm × 300, 5 μm, Thermo Fisher Scientific). Samples were eluted over a 90 min gradient from 0 to 35% acetonitrile at a flow rate of 0.3 μL/min ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples (5 μl aliquot) were normalized to 2-5 nM with Nuclease-free Water (Ambion), then 2 μl from each sample within one 96-index set was pooled to a total of 192 μl at 2-5 nM concentration ...
-
bioRxiv - Cell Biology 2024Quote: ... Peptides were enriched with μ-Precolumn (0.3 mm i.d. × 5 mm, 5 μm, Thermo Scientific) and separated on an AURORA column (0.075 mm i.d ...
-
bioRxiv - Cell Biology 2024Quote: ... 5 mg/ml IgG (Kiovig, Shire) and 5% FcR in Superblock blocking buffer (Thermo Fisher) at room temperature for 2 h ...
-
bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled on intrinsic cysteines using 5-IAF dye (5-iodoacetamido-fluorescein, Thermo Fisher). Before labeling ...
-
bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
-
bioRxiv - Bioengineering 2019Quote: ... The cells were heat shocked in 50 μL (OD600 = 0.04) and transferred to plates (Nunc 96-Well Optical-Bottom Plate) containing 150 μL of their respective medium (final of OD600 = 0.01) ...
-
bioRxiv - Microbiology 2020Quote: ... 10μl of lysate from the cell culture plate mixed then was transferred to a white Nunc 96-microwell plate (ThermoFisher) and 100μl of luciferase assay reagent (Promega Kit ...
-
bioRxiv - Biochemistry 2022Quote: ... GTPase assays were performed in 200µl volumes in 96-well plates and the release of inorganic phosphate (Pi) was monitored at 360nm using the MultiSkan Go plate reader (ThermoFisher). Assays were performed in triplicates and a reference data set containing reaction buffer substrate (MESG ...
-
bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
-
bioRxiv - Bioengineering 2019Quote: ... the cells sampled at the same time points and diluted in PBS had their OD and YFP fluorescence (488nm excitation and 527nm emission) measured on a SynergyMX microtiter plate reader using clear-bottomed black microtiter plates (Thermofisher), and fluorescence values normalized to sample OD following the subtraction of background fluorescence from CEN.PK 113-7D ...
-
bioRxiv - Synthetic Biology 2020Quote: Transformants were selected by plating first on YPD plates and then replica plating to yeast agar plates including 600 mg/L geneticin (Gibco) and incubating for a further 16 hours.
-
bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
-
bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
-
bioRxiv - Developmental Biology 2022Quote: ... OPCs were seeded at 40,000 cells per well in 96-well plates or 50 million cells per 500 cm2 bioassay plate (166508, Thermo Fisher) (for generating large quantities of cells for sequencing studies ...
-
bioRxiv - Neuroscience 2022Quote: ... col seeded NGM plates and then moved to OP50 seeded plates containing 10 µM ThermoFisher scientific LysoTracker Blue DND-22 (Invitrogen) (1mM stock diluted 1:100 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 100 µL of culture with 26’000 cells was transferred in each well of the plate Nunc Edge 96-well plate (Thermo Scientific). The transfection mixture was added to the cells once it has incubated for approximately 30 min.
-
bioRxiv - Cell Biology 2024Quote: HeLa cells were seeded at a density of 15,000 cell/cm2 in either 24-well plates or 12-well plates on #1.5 glass coverslips (Thermo Fisher Scientific). The following day ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Fifty hydras were individually maintained at 18°C in cell culture plates (12-well plates, 1.5 mL/well, Thermo Scientific) filled with Volvic© water under a photoperiod of 12h ...
-
bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Two microliters of each CFPS reaction were diluted with 33 μL of phosphate-buffered saline and analyzed in triplicate on a black 384-well plate with a multimode plate reader Varioskan Lux (ThermoFisher) at 488 nm excitation and 535 nm emission.
-
bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
-
bioRxiv - Cell Biology 2023Quote: ... In a next step 25µl of the compound cell culture media mix were carefully transferred from the intermediate plate on plates with the cells using a Beckman Coulter Biomek Fx followed by incubation in the Cytomat incubator (ThermoFisher) at 37°C and 5% CO2 for 24 and or 72 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the supernatants were transferred to a UHPLC-adapted 96-well plate (Nunc 96-well V-bottom polypropylene plate, ThermoFisher) and sealed ...
-
bioRxiv - Molecular Biology 2024Quote: ... 7.5 μM sgRNA stock plates were thawed and 5.76 μl of each guide was transferred to a 96 well conical bottom plate (Nunc, 249935) using a CyBio SELMA ...
-
bioRxiv - Immunology 2021Quote: Secreted IL-10 was determined by ELISA as per the manufacturer’s protocol (Mouse IL-10, Invitrogen, No: 88-7105-88). The color reaction was measured as OD450 units on a Bio-Rad iMark microplate reader ...
-
bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2019Quote: To measure the levels of secreted IL-17a from the Th17 cell culture assay we utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions.