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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were probed with rabbit anti-p239 polyclonal antibodies and Alexa Fluor®555-labeled goat anti-rabbit IgG (Thermo Fisher Scientific). Cellular nuclei were counterstained with 4□ ...
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bioRxiv - Biochemistry 2023Quote: ... ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher, 4:1 beads/cell).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice and incubated with an anti-mouse secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, # A11001) at a 1:1000 dilution in PBS-T ...
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bioRxiv - Biophysics 2023Quote: ... Isolated T cells were then activated and expanded using magnetic microspheres coated with anti-CD3/CD28 antibodies (Dynabeads; Thermo Fisher, MA, USA) according to the manufacturer’s protocol for 72 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The sorted CD8+ cells were then cultured in RPMI-160 medium supplemented with 10% FBS and activated with anti-CD3 antibody (Thermo Fisher Scientific), anti-CD28 antibody (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... the transfected cells were harvested and stained with Alexa Fluor 647-anti-SARS-CoV-2 RBD antibody (51-6490-82; Thermo Fisher Scientific). Ancestral and variant spike expressing HEK293T cells were sorted for as target cells using the FACSAria™ Fusion Cell Sorter (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) for visualization of host-cell nuclei and anti-MOMP antibody conjugated to FITC (Thermo Scientific™) for visualization of Chlamydia ...
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bioRxiv - Cancer Biology 2023Quote: ... trypsinized cells were incubated with phycoerythrin-labeled UIC2 antibody or phycoerythrin-labeled isotype control IgG2a kappa (both from ThermoFisher, Grand Island, NY) for 20 min at room temperature in 2% bovine serum albumin/PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 100ul of resuspended cells in FACS buffer were incubated with 1ul APC labelled Sca-1 antibody (Thermofisher scientific, Catalog # 17-5981-82) for 20 mins on Ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were centrifuged at 1400 rpm for 4 minutes and incubated with Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (ThermoFisher, MA5-14520) at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... After counting in hemocytometer cells were labeled with mouse monoclonal antibodies using the markers live/dead Aqua (Life Technologies, Carlsbad, CA, USA), -CD45 ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... the cells were washed 3 times with DPBS and incubated with secondary goat anti-rabbit antibody (Alexa Fluor 488; Thermo Fisher Scientific) at a 1:500 dilution in 1X PBS containing 1% bovine serum albumin and 0.3% Triton X-100 for 1-1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... or 10 µg of mouse IgG1 isotype control antibody (Cell Signalling Technology, #5415) in binding buffer consisting of CEB fractionation buffer supplemented with HALT phosphatase inhibitors (Thermo Fisher Scientific) and cOmplete proteinase inhibitors (Roche ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with 5 µg of either the targeted antibodies (SRSF3: Cell Signaling Technology #35073; SRSF7: Bethyl A303-772A; PTBP1: ThermoFisher, 32-4800 ...
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bioRxiv - Immunology 2024Quote: ... diluted antibodies against cell surface proteins (see Supplementary Table 2) and a viability dye (LIVE/DEAD Fixable Blue, Thermo Fisher Scientific, #L23105) were added ...
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bioRxiv - Neuroscience 2024Quote: ... For quality controls, PSCs passed array CGH testing (Cell Line Genetics, Madison, WI) and pluripotency testing using a four-antibody kit (Thermo Fisher #A24881). Cell culture media were sterile filtered (Fisher Scientific #SCGP00525 and #0974063D) ...
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bioRxiv - Bioengineering 2024Quote: ... the cells washed three times in ice-cold PBSA were incubated with goat anti-mouse Alexa Fluor 488 antibody (Invitrogen, 2mg/mL) and anti-His6x monoclonal antibody conjugated with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The following secondary antibodies were used at a 1:1000 dilution for cultured cells: donkey anti-rat Alexa Fluor 488 (Invitrogen A-21208) and donkey anti-mouse Alexa Fluor 488 (Invitrogen A-21202) ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed with PBS and incubated with Alexa Fluor 555-conjugated anti-rabbit antibody (1:1000 dilution, A21428; Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated for 2 hours at room temperature with Alexa 488/568-conjugated secondary antibodies (1:1000: Invitrogen, MA, USA). Nuclei were stained for 5 minutes with 1 µM 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation at room temperature cells were washed twice with PBS and FITC conjugated secondary goat anti-rabbit polyclonal antibodies (Thermofisher; Cat # 31635) were added ...
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bioRxiv - Cell Biology 2024Quote: ... The following were added to the cell culture medium: a 1:5000 dilution of Alexa FluorTM 647-conjugated Rabbit anti-GFP antibodies (Thermofisher, A-31852), 0.1 µM thapsigargin (Calbiochem ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with PBS and incubated with Alexa Fluor conjugated secondary antibodies (Jackson ImmunoResearch or ThermoFisher, 1:400 dilution) diluted in 5% donkey serum for one hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then rinsed with PBST and incubated with Alexa Fluor-conjugated secondary antibodies (1:500; 488 or 594; Thermo Fisher Scientific) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... A quantitative PCR-based titration method 37 was used to determine an encapsulated vector genome titer utilizing a Prism 7500 Fast Taqman detector system (PE Applied Biosystems Grand Island, NY USA). The following primers/probes were used ...
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bioRxiv - Immunology 2021Quote: ... CD4-APC (RPA-T4) and CD45RO-PE (UCHT1) (both from Becton Dickinson, Franklin Lakes, NJ, USA) and CD8-Pacific Orange (3B5) (from Thermo Fisher Scientific, Waltham, MA, USA). Cells were stained at room temperature for 20 min and sorted with FACSAria II (Becton Dickinson) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant sample of a pJPZ11 culture grown for 5 days in standard condition was concentrated ~40 fold by filtration using a pierce protein concentrator (Pierce™ Protein Concentrator PES, 10K MWCO, 5-20 mL, Thermo Fisher Scientific, IL, USA) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... before a final elongation step at 72 °C for 10 min using a GeneAmp 9700 PCR System (Applied Biosystems, PE Corp., Foster City, CA, USA). PCR products were purified using ExoSAP-IT (Affymetrix ...
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bioRxiv - Immunology 2021Quote: ... was performed using IAV-NP mAb (IAV-NP HB-65) as primary antibody and Alexa fluor 594 goat anti-mouse antibody as secondary antibody (Invitrogen, catalog no: A11005). SIV infected cells were identified based on the presence of fluorescent foci.
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bioRxiv - Cancer Biology 2020Quote: ... peroxidase-linked species-specific whole antibody (from sheep) secondary antibody (45-000-679, 1:5000-50000 depending on the primary antibody) were both from Fisher Scientific (Waltham, MA). Immunoblots were imaged using Immobilon Western Chemiluminescent HRP Substrate (ECL ...
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bioRxiv - Microbiology 2022Quote: ... the cells were stained overnight at 4 °C with E1 or nsP1 primary rabbit antibodies (both antibodies previously validated in our laboratory) [11] followed by Alexa Fluor 568 goat anti-rabbit secondary antibody (Invitrogen, Carlsbad, CA, USA). Finally ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488; Molecular Probes, Karlsruhe, Germany) to localize CDA proteins or chitin respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with corresponding alkaline phosphatase-coupled secondary antibodies (goat anti-rabbit polyclonal antibody, Thermo Scientific, 1:10000; or goat anti-mouse polyclonal antibody, Invitrogen, 1:10000). The presence of the antigens of interest was observed with Enhanced Chemifluorescence substrate (ECF ...
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bioRxiv - Neuroscience 2024Quote: ... or HRP-conjugated secondary antibodies (Chicken anti Mouse secondary antibody HRP, Fisher Scientific # A15981, Sheep anti Rabbit secondary antibody HRP, Fisher Scientific #A16172) at RT for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thoroughly with cold PBS to remove unbound primary antibody and a secondary antibody (Alexa 488 goat anti-mouse IgG secondary antibody (Invitrogen, OR Waltham, MA)) at a 1:750 dilution in PBS was applied to the samples for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membrane was incubated with mouse anti-P72 antibody or mouse anti-HA antibody and HRP conjugated anti-mouse secondary antibody (Invitrogen, Cat#62-6520). Anti α-actin HRP Antibody for protein loading control was purchased from Santa Cruz Biotechnology (sc-47778 HRP).
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bioRxiv - Cancer Biology 2021Quote: ... we used the following primary antibodies: mouse anti-V5 monoclonal antibody (46-0705, Invitrogen), rabbit anti-FOXA1 M2 polyclonal antibody (GTX100308 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies were detected with Alexafluor-594 and Alexafluor-488-conjugated secondary antibodies (Invitrogen). Nuclei were stained with DAPI and cells were imaged using the Zeiss LSM700 system.
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were incubated with primary antibodies (PTPRH antibody, 1:1000, Fisher Scientific, #PIPA531340) overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Antibody detection was performed using AlexaFluor (488 and 568) conjugated secondary antibodies from Invitrogen at 1:800 dilution in blocking solution overnight at 4°C followed by washes with 1% Tween-20 in PBS.
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bioRxiv - Cancer Biology 2021Quote: ... The GFP antibody was paired with Alexa 647-conjugated anti-chicken secondary antibody (Invitrogen) to avoid confounding tissue autofluorescence ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were Alexa Fluor-488 and −555 conjugated antibodies (1:1000, Molecular Probes) and goat anti-Chicken Alexa Fluor-488 (1/800 ...
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bioRxiv - Molecular Biology 2021Quote: Antibodies for immunoblots: primary antibodies included anti-myc monoclonal 9E10 (Thermofisher, diluted 1:10,000) or anti-G6PDH (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody: Goat anti-mouse Alexa-488 secondary antibody (Invitrogen, A11034, 1:500) and goat anti-mouse Alexa-647 secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies used: AlexaFluor 405-labeled goat anti-mouse antibody (1:500; ThermoFisher #A31553). Actin was stained by incubation (30-120min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary Alexa Fluor antibodies and HRP conjugated secondary antibodies were procured from Life technologies.