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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells are then washed three times with PBS before incubated with Alexa FluorTM 555-conjugated α-mouse secondary antibody (Invitrogen #A28180) at 1:2000 in permeabilization/blocking buffer for 1 h at room temperature ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: Plasmids carrying the antibody heavy and light chain of nAbs were used for transient transfection of Expi293F™ cells (Thermo Fisher) as previously described22 ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Biochemistry 2022Quote: ... The Ndc80-antibody and cell suspension were mixed and then the mixture was loaded into a 100 μl Neon Pipette Tip (Thermo Fisher). Electroporation was performed at 1005 V with two consecutive pulses of 35 msec ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed once with PBSB and incubated with goat anti-rabbit antibody labeled with Alexa Fluor 488 (Invitrogen A11008) diluted 1:100 in PBSB for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed and stained with the appropriate secondary antibodies: Alexa Fluor 594 (red) goat anti-rabbit (A11012, Thermo Scientific), Alexa Fluor 488 (green ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated at RT for 1 hr with an anti-mouse secondary antibody tagged with AlexaFluor488 (Thermo Fisher Scientific, A11001) used at 2 μg/mL in blocking buffer ...
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bioRxiv - Physiology 2022Quote: ... All surface and total staining cells were incubated with secondary antibody at 1:1000 in diluted superblock with Hoechst 33342 (Cat. No. LSH3570, Invitrogen Molecular Probes) at 1:2500 dilution in the dark for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... cells were rinsed and incubated with the secondary antibody conjugated with Alexa Fluor® 594 (anti-mouse; 1:500, Molecular Probes). Cell nuclei were counterstained with DAPI (0.2 mg/ml in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated with a 1:500 dilution of Alexa Fluor 488 secondary antibody (A11029, Thermo Fisher, MA, USA) for 45 min at RT and then washed three times with PBST for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... and 293T cells were incubated with 100 μl Alexa Fluor® 647-labeled anti-rabbit antibody (2 μg/ml, Thermo Scientific) for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... blocked with 1 % BSA in PBS and stained with antibody against anti-MYC (#2276, cell signaling) at 1:1000 and Isotype control Mouse IgG2a (#14-4724-82, ThermoFisher scientific) at 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated overnight at 4°C with a primary antibody directed against the V5-tag (ThermoFisher, 46-0705, 1:200) or against small double-stranded RNAs (J2 antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed three times and incubated with Alexa 488/647-labeled anti-mouse/rabbit secondary antibody (Invitrogen, A21202, A31573) for 1h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (Table S1) were incubated for 1 hour and cell nuclei were counterstained using DAPI (Thermo Fisher Scientific; Cat# 62248).
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bioRxiv - Cancer Biology 2024Quote: ... HM and MeT5A cells were pre-incubated with 10 μg mL−1 P-cadherin antibody (NCC-CAD-299) (Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Lung cell suspension was then subjected to positive sorting with an antibody against the endothelial cell marker ICAM-2 conjugated to magnetic Dynabeads Sheep Anti-Rat IgG (11035) from Thermo Fisher, for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated with the secondary antibody (goat anti-mouse/-rabbit Alexa 488 or 568; 1:200; Thermo Fisher Scientific) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with PBS followed by incubation with the secondary antibodies goat anti-mouse DyLight594 (1:250, Invitrogen Cat#35561) and goat anti-rabbit STAR635 (1:250 ...
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bioRxiv - Microbiology 2024Quote: ... cell lysate (30 μg) were analyzed by western blotting using the following antibodies: rabbit anti-MAVS (Invitrogen, PA5-17256, 1:1000), rabbit anti-IRF3 (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... protein lysate was extracted from tumor cell lines as described above and used as input for Western blots with anti-KRAS antibody (12063-1-AP; Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... The isolated cells were washed with ice-cold PBS again and centrifuged to collect the cell pellets for antibody labeling in a fluorescent washing buffer (Thermo Fisher). The cell suspension was incubated with BV421 anti-mouse CD11b ...
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bioRxiv - Immunology 2024Quote: ... The non-parenchymal cell layer was then removed and LSECs were isolated via positive selection using CD31 antibody-conjugated DynabeadsTM (Invitrogen, RRID:AB_3661738). LSECs were resuspended in human endothelial serum-free medium (SFM ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were subsequently analyzed using an Alexa Fluor 488-conjugated goat anti-mouse IgG monoclonal antibody (1 μg/mL, Invitrogen, USA). The analysis of stained leukocytes was conducted with a CytoFLEX flow cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for one hour in primary antibody NPM1 (FC-61991, ThermoFisher, 1 : 500 dilution in PBST with 1 %BSA) ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were stained with rabbit α-desmin and respective secondary antibody (Supplementary Table 2) and Hoechst 33342 (Thermo Fisher Scientific), and imaged using an ImageXpress Pico Automated Cell Imaging System (Molecular Devices) ...
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bioRxiv - Developmental Biology 2024Quote: ... EdU was detected after secondary antibody incubation using the Click-iT™ EdU Cell Proliferation Kit for Imaging and Alexa Fluor 488 dye (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with PBS and incubated with goat anti-rabbit secondary antibody (Alexa Fluor 633, Invitrogen, A-21070) diluted 1:500 in blocking solution for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates from wild type cells and capture of eIF4E3 and eIF4E4 using affinity-purified rabbit antibodies coupled to magnetic beads Dynabeads™ Protein A (Thermofisher). A commercial ...
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bioRxiv - Microbiology 2024Quote: ... and cells were directly incubated with primary antibody BCoV-Nucleocapsid mouse anti-bovine monoclonal (Invitrogen, Clone: FIPV3-70; Cat. # MA1-82189) diluted in blocking solution at 1:100 for overnight at 4°C in dark ...
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bioRxiv - Microbiology 2024Quote: ... Capsule acquisition was visualized by incubating the cells with an anti-GXM (18B7) antibody conjugated with AlexaFluor 488 (ThermoFisher Scientific, USA) for 1 h at 37 °C and observing under an Eclipse Ti-E fluorescence microscope (Nikon ...
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bioRxiv - Biochemistry 2024Quote: ... cells were resuspended in pre-warmed (37°C) media containing anti-cytokine blocking antibody (AB12-3G4, ThermoFisher Scientific 16-7027-85) at a final concentration of 20 ug/mL and incubated at 37°C for the following time points ...
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bioRxiv - Cancer Biology 2024Quote: ... cell surface molecules of DLK1 or DLL3 per cell were calculated after subtracting background signal from DLK1 secondary antibody alone (Invitrogen, #P852) or DLL3 isotype control antibody (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Next day cells were incubated with Alexa Flour™ 488-conjugated or Alexa Flour™ 568-conjugated secondary antibody (Thermo Scientific) in 0.2% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the cells are washed three times with 1× PBS and incubated with secondary antibody anti-mouse AlexaFluor 546 (1:400 Thermo Fisher) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently immunoreacted with the primary antibodies (Mouse anti-Calbindin antibody, Abcam, ab82812, 1:200; Chicken anti-GFAP antibody, Thermo Fisher, PA1-10004 ...
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bioRxiv - Physiology 2020Quote: Secondary antibodies used were donkey anti-rabbit IgG secondary antibody and goat anti-mouse IgG secondary antibody (both from Thermo Scientific). AlphaView Software (Protein Simple ...
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bioRxiv - Developmental Biology 2023Quote: ... Excess antibody was washed off using PBSTx followed by incubation with secondary antibody against the respective primary antibody host (Molecular Probes; Alexa 488 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Biochemistry 2024Quote: ... phospho-V2R [pT359/pT360] antibody (1:2000 dilution, 7TM antibodies) followed by HRP-coupled anti-rabbit secondary antibody (1:5000 dilution, Thermo Fisher) for 1 hour each ...
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bioRxiv - Plant Biology 2020Quote: ... first with Whatman® filter paper grade 1 (Whatman International Ltd, Maidstone, UK) and subsequently with a sterile 0.2 µm PES syringe filter (Thermo Fisher Scientific, Waltham, MA, USA), to separate broth and fungal biomass ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 10^6 cells were washed with PBS supplemented with 0.5% BSA and 0.1% sodium azide and stained with FITC-conjugated anti- CD5 (eBioscience, cat. 11-0051-85) and with PE-Cy7-conjugated anti-CD19 (Invitrogen , cat. 25-0193-82) antibodies ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Immunology 2020Quote: ... For the last incubation beads were resuspended in 50 μl of Stabilguard and 50 μl of streptavidin-phycoerythrin (SA-PE) (Molecular Probes, Leiden, The Netherlands) was added ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: MHC-I tetramer targeting the immunodominant epitope of the influenza nucleoprotein (DbNP366-374 – ASNENMETM, DbPA224-233 - SSLENFRAYV) was produced in-house and conjugated to streptavidin-APC/PE (Life Technologies Australia Pty Ltd) at a 1:250 dilution at room temperature for 1h ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a 50-fold dilution of PE-conjugated goat anti-human IgM or IgG (both from Thermo Fisher, Waltham, MA, US) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The filtered supernatants were concentrated 8-fold using a pre-rinsed 3K MWCO Pierce Protein Concentrator PES (molecular-weight cutoff: 3 kDa) (Thermo Fisher Scientific, 88525, USA) by centrifuging at 4000 rcf in 4°C ...