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Citations for Thermo Fisher :
3201 - 3250 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Peptide extraction was performed using the EasyPep™ MS Sample Prep Kits (Thermo Fisher Scientific Inc, Massachusetts, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos tribrid mass spectrometer with an ESI source (ThermoFisher Scientific) coupled online to the nano-LC and operated in positive polarity ...
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bioRxiv - Cancer Biology 2024Quote: ... suspended in Formic Acid (FA) 0.1% and dosed with the quantitative fluorometric peptide assay (ref. 23290, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated by reversed-phase chromatography using a 50-cm MicroPac Nano C18 column (Thermo Fisher Scientific) with mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile ...
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bioRxiv - Microbiology 2024Quote: TMT-labelled tryptic peptides were subjected to HpRP fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher) equipped with a 2.1 mm internal diameter x 25 cm long ...
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bioRxiv - Microbiology 2024Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific). All databases include a reversed version of all the sequences ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were analysed by liquid chromatography coupled to mass spectrometry (LC-MS) using a Easy1200 nLC (Thermo Scientific) coupled to a tribrid Orbitrap Eclipse with FAIMSpro mass spectrometer (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Tagged ribosomes were then eluted by incubating the beads with 200 µg/mL HA peptide (Thermo Fisher Scientific) for 15 minutes at 30°C with constant agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides directly sprayed into an Orbitrap Fusion Lumos mass spectrometer equipped with a nanoFlex ion source (ThermoFisher Scientific). Full scan MS spectra (350-1400 m/z ...
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bioRxiv - Synthetic Biology 2024Quote: LC-MS/MS analyses of the digested peptides were performed on an EASY-nLC 1200 (Thermo Fisher Scientific) coupled to a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Acidified eluted peptides were analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) in the data-dependent mode ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were analysed by nano HPLC-MS/MS with a Ultimate 3000 nanoRSLC (Thermo Fisher Scientific, Darmstadt, Germany) operated in a two- column setup (2 cm PepMap C18 trap ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS before staining with primary antibodies diluted in blocking buffer and a mixture of 1:1000 of Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen), 1:5000 Dapi (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μm thick vibratome sections were postfixed with 4% PFA for 15 min at RT followed by blocking in 10% normal horse serum (Gibco) and 0.2% Triton in PBS overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... we incubated the samples in blocking solution with fluorescent dye-conjugated secondary antibodies and the nuclear counterstain DAPI (ThermoFisher Scientific) if applicable at 4 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Developmental Studies Hybridoma Bank) was used at 1:30 in blocking solution and goat α-mouse Alexa568 secondary antibody (Invitrogen) at 1:1,000 in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were washed three times with 0.05% PBST and loaded with a blocking solution of 0.5% BSA (Fisher Scientific, BP9706100) in 0.1% PBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Stained platelets were then washed in blocking buffer and mounted on poly-L-ornithine-coated glass slides using ProLongTM Glass Antifade mountant (Invitrogen), covered with coverslips and stored at 4 °C until imaging by multiphoton microscopy.
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 4X in PBS with .5% Triton X-100 (PBS-T) and then placed in blocking solution (PBS-T with 5% normal goat serum (NGS; Invitrogen)) for 90 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed three times with 1X PBS for 5 minutes each and blocked for 30 minutes at RT with Blocking Buffer [0.4% acetylated BSA (Ambion, AM2614) in 4X SSC] in a humidified chamber ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions with primary antibodies were performed in blocking buffer containing rabbit anti-GFP (1:1000, Invitrogen, Carlsbad, CA, USA, A11122) and/or goat anti-c-Fos (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Triton X-100 for 5 min and incubated with a blocking buffer containing PBS and 5% normal goat serum (31873; Invitrogen) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in Odyssey Blocking Buffer containing HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) or anti-mouse IgG (H+L ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed three times with 0.01% Triton X-100 in PBS for 5 minutes each and then incubated in blocking solution containing corresponding secondary antibodies labeled with Alexa fluorophores (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... All stains were carried out in media containing anti-CD16/32 blocking antibody (ThermoFisher, clone 93, catalog # 14-0161-86). All flow cytometry was acquired on an LSRFortessa FACS analyzer ...
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bioRxiv - Genetics 2019Quote: ... was applied at a final concentration of 1:300 in blocking buffer for 1 hr and Hoechst 33342 (ThermoFisher #H3570) applied for 1-5 min at a concentration of 1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000 ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was in-cubated for 1 h at room temperature with Pierce Protein-Free Blocking Buffer (Thermo Fisher Scientific) and again washed with PBS three times ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were washed six times with blocking solution and then incubated for two hours with Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen, USA ...
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bioRxiv - Biochemistry 2020Quote: ... After transfer and blocking of polyvinylidene fluoride (PVDF) membranes (Immobilon) in 5% BSA TBS Tween-20 buffer (Thermo Fisher Scientific), primary antibody directed against FADS2 (PA5-87765 ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were washed with PBS and incubated overnight at 4° C with blocking buffer (Thermo Scientific, Waltham, MA, cat. #37572) to eliminate nonspecific sites ...
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bioRxiv - Bioengineering 2020Quote: ... After incubation with excess streptavidin and biotin to block endogenous biotin (Endogenous Biotin Blocking Kit; Thermo Fisher Scientific, Waltham, MA), 5 µM of biotinylated HCDR3-construct was incubated on tissue overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were blocked in Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (Mouse anti-V5 antibody, Invitrogen R960-25 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 min followed by incubation with secondary antibodies diluted 1:500 in blocking buffer: goat anti-Chicken Alexa Fluor®546 (RRID: AB_2534097, ThermoFisher) and Goat anti-Rabbit Alexa Fluor®488 (RRID:AB_2534096 ...
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bioRxiv - Physiology 2021Quote: ... Brain sections were then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies diluted 1:1000 in blocking buffer (donkey anti-mouse Alexa 488, donkey anti-rabbit Alexa Fluor 555 or 647, Invitrogen) were applied for 4 hours at room temperature with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in blocking solution containing secondary antibodies for 24 hr (streptavidin AlexaFluor conjugate, Invitrogen; 1:250 goat anti-mouse AlexaFluor conjugate, Invitrogen). Following staining ...
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bioRxiv - Cell Biology 2021Quote: ... secondary labeling was performed by addition of the following antibodies diluted in blocking/ permeabilization buffer for 30 minutes at room temperature: Alexa Fluor 647 or 488 donkey anti-mouse (Invitrogen), Alexa Fluor 647 or 488 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was crosslinked using a UV stratalinker and blocked at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Ambion) for 2 hours while rotating ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:100 in blocking buffer and the secondary antibody (Anti-mouse IgG conjugated with Alexa 635, Goat IgG - Invitrogen) diluted 1:300 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... The wells were then washed 5 times with phosphate-buffered saline (PBS) + 0.05% Tween 20 and blocked with SEA BLOCK blocking buffer (Thermo Scientific) in the first panning round and 5% milk powder in PBS in the second panning round ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene, OR, US; Donkey anti-Mouse, AlexaFluor 488, A21202, Invitrogen, Eugene ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated for two hours with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody against phospho-AKT and total-AKT and Alexa Fluor 596-conjugated goat anti-mouse secondary antibody for PRMT5 (diluted 1:100 in blocking buffer, Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...