Labshake search
Citations for Thermo Fisher :
3101 - 3150 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... eluted peptides were directly sprayed onto the Q Exactive HF Orbitrap LC-MS/MS system (Thermo Fisher Scientific), using the nanoelectrospray interface ...
-
bioRxiv - Molecular Biology 2022Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100, 5 μM, 5 x 0.3 mm, ThermoFisher Scientific) at a flow rate of 10 uL/min using 2% ACN and 0.05% TFA as loading buffer ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... desalted peptides were injected into a Thermo LTQ Orbitrap XL Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) or an Orbitrap Elite™ Hybrid Ion Trap mass spectrometer ...
-
bioRxiv - Systems Biology 2024Quote: ... Approximately 0.6 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, ThermoFisher Scientific) and separated on a PepMap RSLC C18 column (50 cm × 75 μm ...
-
bioRxiv - Systems Biology 2024Quote: ... peptide mixtures were separated by a reversed-phase chromatography (Dionex Ultimate 3000 NCS-3500RS Nano, Thermo Fisher Scientific) coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... but peptides were gradient eluted from the column directly to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher) using a 155 min acetonitrile gradient from 5 to 35 % B in 120 min followed by a ramp to 45% B in 10 min and 100% B in another 10 min with a hold at 100% B for 10 min (A=2% acetonitrile in 0.5% acetic acid ...
-
bioRxiv - Cancer Biology 2024Quote: Peptide samples from a 24-hour experiment were injected on a Dionex Ultimate 3000 RSLC (Thermo Fisher Scientific) connected to an Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2024Quote: ... 100 μg peptide mixture for each sample was labeled using TMT reagent according to the instructions (Thermo Scientific). The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... The membrane permeability of the peptide was determined by using the LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen). S ...
-
bioRxiv - Microbiology 2023Quote: ... biotinylated Pol II CTD mimic peptide (20 µg) were immobilised on 10 µl streptavidin agarose resin (Thermo Scientific) for 2 hours at 4 °C in binding buffer (10 mM HEPES [PAA catalogue no ...
-
bioRxiv - Microbiology 2024Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... The peptides generated were analyzed by electrospray ionization on an Elite tandem orbitrap mass spectrometer (Thermo Scientific Inc). Nanoflow HPLC was performed by using a Waters NanoAcquity HPLC system (Waters Corporation) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Peptides were ionized using a nanospray ion source into a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). MS/MS spectra were searched against the Uniprot human complete proteome FASTA database downloaded on 2018_10_26 ...
-
bioRxiv - Genomics 2024Quote: ... around 1 μg of total peptides were analyzed on an EASY-nLC 1000 UHPLC system (Thermo Fisher Scientific) coupled via a 20 cm C18 column ID picofrit column (New Objective ...
-
bioRxiv - Neuroscience 2024Quote: Tryptic peptides were analysed by LC-MS/MS using an EASY-nLC system (Proxeon Biosystems, Thermo Fisher Scientific) connected to a Velos-Orbitrap mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...
-
bioRxiv - Molecular Biology 2024Quote: 800 ng of peptides were analyzed on a Q-Exactive Plus mass spectrometer (Thermo Scientific, San Jose, CA) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... 90% acetonitrile in water) was employed for peptide separation on an ASY-nLC 1200 UPLC system (ThermoFisher Scientific). The separation gradient was set as follows ...
-
bioRxiv - Molecular Biology 2024Quote: ... Eluates were combined prior to fractionation with the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Scientific). The dried TMT-labelled phosphopeptide fractions generated were resuspended in 0.1% TFA for LC-MS/MS analysis using a U3000 RSLCnano system (Thermo Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... The peptide digests were frozen at −80°C and dried by vacuum centrifugation (Savant SPD121P, Thermo Fisher Scientific), reconstituted in 0.07% trifluoroacetic acid ...
-
bioRxiv - Plant Biology 2024Quote: ... and peptide samples were eluted with 0.1% formic acid and analysed on an Orbitrap Elite instrument (ThermoFisher Scientific) [38] attached to an EASY-nLC 1000 liquid chromatography system (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on a 2 cm nanoviper Precolumn (i.d. 75 μm, C18 Acclaim PepMapTM 100, Thermo Scientific) at a flow rate of 3.0 µL/min in buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
-
bioRxiv - Neuroscience 2024Quote: ... Peptide concentration employed a trapping column (Acclaim C18 PepMap100, 100 μm, 2 cm, Thermo Fisher Scientific, Schwerte, Germany) with 0.1% trifluoroacetic acid (TFA ...
-
bioRxiv - Biochemistry 2024Quote: ... The TMT-set was further purified using High Protein and Peptide Recovery Detergent Removal Spin Column (88305, ThermoFisher) and Pierce peptide desalting spin columns (89852 ...
-
bioRxiv - Physiology 2024Quote: ... The eluting peptides were analyzed on a quadrupole orbitrap ion trap tribrid mass spectrometer (Fusion, Thermo Fisher Scientific) in data dependent acquisition (DDA ...
-
bioRxiv - Immunology 2024Quote: ... The deep-fractionated peptide samples were separated on an online nanoflow Easy-nLC-1200 system (Thermo Fisher Scientific) and analyzed on Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: Peptides were analyzed on a Waters NanoAquity UPLC system coupled with a Q-Exactive mass spectrometer (Thermo Scientific), as previous described in detail [13] ...
-
bioRxiv - Biochemistry 2024Quote: Phox-crosslinked peptides were enriched prior to high-pH fractionation using a Fe-NTA magnetic resin (Thermo Scientific). Peptides were resuspended to a concentration of 1mg/mL in 80% acetonitrile (MeCN ...
-
bioRxiv - Biophysics 2024Quote: ... The resulting peptide-mRNA-DNA fusions were then incubated with PfUCLH3 immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 4 °C ...
-
Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted over a 44-min gradient and analysed with an Orbitrap EclipseTM TribridTM instrument (Thermo Fisher) with FAIMS ProTM Interface (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... dried peptides were reconstituted in 0.1% formic acid and analyzed on a Dionex Ultimate3000 nano HPLC (Thermo Scientific) coupled to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the resulting peptides were analyzed by LC-MS/MS on a Q-Exactive MS instrument (Thermo Scientific). Peptides were separated by nano-LC using an UltiMate 3000 high performance liquid chromatography and autosampler system (Dionex ...
-
bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
-
bioRxiv - Neuroscience 2023Quote: ... Peptide concentrations in mass spectrometry ready samples were measured using the mBCA assay (Thermo Scientific™ Pierce™). Three stable isotope labeled reference peptides were spiked into the final peptide samples at known concentrations (Vivitide ...
-
bioRxiv - Plant Biology 2023Quote: ... and peptide samples were resuspended in 0.1% FA solution and analysed on an Orbitrap Elite instrument (ThermoFisher Scientific) (Michalski et al. ...
-
bioRxiv - Systems Biology 2023Quote: ... Peptides were initially separated using a Dionex UltiMate 3000 UHPLC system on an EasySpray C18 column (Thermo Scientific, OD 360 µm ...
-
bioRxiv - Systems Biology 2023Quote: ... peptide digests were analyzed in DDA and DIA modes on a Thermo Scientific Eclipse mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
-
bioRxiv - Biochemistry 2022Quote: ... The peptides were identified by ESI mass spectrometry (MS) performed on an LCQ Fleet Ion Trap (Thermo Scientific) mass spectrometer ...
-
bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
-
bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
-
bioRxiv - Molecular Biology 2023Quote: ... The peptides mixtures were analyzed by nano-LC-MS/MS using an Ultimate 3000 system (Thermo Fisher Scientific) coupled to an LTQ-Orbitrap Velos mass spectrometer ...
-
bioRxiv - Molecular Biology 2023Quote: All peptide samples were separated with an online nanoflow Proxeon EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific) and analyzed on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Online detection of peptide ion was by electrospray ionization mass spectrometry using an Orbitrap Elite MS (Thermo Scientific). Peptides were separated on a PepmapTM C18 reversed phase column (3 μm ...
-
bioRxiv - Systems Biology 2023Quote: Peptides were labeled by addition of a third of an aliquot of TMT11-131C label reagent (Thermo Scientific) in a final volume of 20% acetonitrile and incubated for 1 hour at 600 rpm and 25°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Separation of peptides by reversed-phase chromatography was carried out on an UltiMate 3000 RSLCnano System (Thermo Scientific) which was equipped with a C18 Acclaim Pepmap100 preconcentration column (100µm i.D.x20mm ...
-
bioRxiv - Microbiology 2023Quote: ... The combined sample was desalted using a Pierce peptide desalting spin column (Thermo Fisher Scientific; catalog no. 89852) and vacuum-dried in a Centrivap (Labconco ...
-
bioRxiv - Immunology 2023Quote: The digested peptides were separated by online reversed phase chromatography on an Dionex UltiMate 3000 (Thermo Fisher Scientific) (column packed with Poroshell 120 EC C18 ...
-
bioRxiv - Bioengineering 2023Quote: ... The purified peptide solution was dried and quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). An equal amount of peptide (∼50 μg ...
-
bioRxiv - Cancer Biology 2023Quote: ... Labeled peptides were fractionated via highpH reverse-phase high-performance liquid chromatography (Thermo Easy nLC1000; Thermo Fisher Scientific) using a precolumn (made in house ...