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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Cell Biology 2023Quote: ... and secondary antibodies (i.e., anti-mouse, 1:3000 and anti-rabbit IgG, 1:4000 conjugated to horseradish peroxidase; Invitrogen 31432/AB_228302 and 31460/AB_228341 ...
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bioRxiv - Neuroscience 2022Quote: ... chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913; 1:1000, rabbit anti-GFP, Invitrogen, Cat# A-l 1122 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-mouse (488, 1:300, Thermo Fisher Scientific, Cat. No. A21022, RRID: AB_141607 or 594, 1:300, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202, 1:800 in 1% BSA/1X PBS) or Alexa Fluor 647 goat anti-rabbit (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... MCPT1 concentration of serum samples was determined using the MCPT-1 (mMCP-1) Mouse Uncoated ELISA Kit from Invitrogen (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were incubated for 1 hr at 37°C with goat anti-mouse AlexaFluor 488 (A11001; ThermoFisher; 1:500) secondary antibody diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were successively incubated with indicated primary antibodies in PBS/BSA 1% saponin 0.1% for 2 h at RT and with Alexa Fluor-conjugated secondary antibodies in PBS/BSA 1% saponin 0.1% for 1 h raised against mouse or rabbit immunoglobulins (Invitrogen). After washes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 5 times with the wash buffer and then incubated at room temperature for 1h with Alexa 488-conjugated chicken anti-mouse secondary antibody together with Alexa-568-conjugated phalloidin (1:500) and Hoechst (1:10,000; Invitrogen). Cells were then washed 8 times with wash buffer and mounted using mowiol (Sigma ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with one of the following secondary antibodies: 1) donkey anti-mouse 488 (1:250; Invitrogen, USA); 2 ...
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bioRxiv - Neuroscience 2023Quote: ... and either AlexaFluor-488/647-conjugated goat anti-mouse or anti-rabbit IgGs (1:400) and Hoechst (1:10,000; Invitrogen). For tissue expansion ...
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bioRxiv - Physiology 2024Quote: ... Alexa Fluor 594 donkey anti-rabbit (1:500) and Alexa Fluor 488 donkey anti-mouse (1: 500; Molecular Probes, Cat# A-21207 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 1 hr incubation in primary antibody solution at room temperature (1:1,000 mouse anti-v5; R96025, Invitrogen). Cells were washed twice with DPBS and incubated with secondary antibody (1:1,000 donkey anti-mouse 568 ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 1 µL mouse anti-V5 primary antibody (1:10,000; R96025, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... before primary antibody incubation (rabbit anti-TH, 1:500, Fisher Scientific and/or mouse anti-GFP, 1:500, Abcam) overnight at 4 °C in PBS with BSA and Triton X-100 (each 0.2%) ...
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Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: ... sections were stained with anti-mouse and anti-rabbit Alexa Fluor-conjugated secondary antibodies (1:500-1:1000, Invitrogen) for one hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... followed by goat anti-mouse IgG-conjugated to Alexa Fluor 647 (ThermoFisher #A21235 1:1000 in PBS+1% BSA) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were added (1:200 Alexa Fluor 488 donkey anti-mouse; 1:200 594 donkey anti-rabbit, Invitrogen) for 2 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were then incubated for 1 h at room temperature with two primary antibodies: mouse anti-6X-HisTaq (dilution 1:1.000, MA1-21315, Invitrogen) and mouse anti-VSV-M (dilution 1:1.000 ...
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bioRxiv - Genetics 2024Quote: ... Membranes were then incubated for 1 hour at 25°C in 1:8000 goat-anti-mouse IgG (#31430, Invitrogen) HRP-conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed with PBST and blotted with secondary antibodies (1:10,000 anti-mouse, Invitrogen, 32230, or 1:10,000 anti-rabbit, Invitrogen, 32260) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva).
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva) ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2023Quote: ... A secondary antibody was used in TBS-T with a dilution of 1:5,000 for Anti-Mouse (Thermo Fisher, Goat anti-Mouse IgG, #31430) and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Iowa City, IA), mouse anti-Futsch (22C10 DSHB, Iowa City, IA) and mouse anti-GFP at 1:1000 (#A-11120, Thermo Fisher Scientific, Waltham, MA). Secondary antibodies used in this study were goat anti-mouse Alexa Fluor 488-conjugated IgG at 1:500 (#A-32723 ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1 μg ml−1 doxycycline (Invitrogen). Expression of Myc-Cdk11 was also induced with 1 μg ml−1 doxycycline in the subsequent experiments.
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP and SYPRO orange protein gel stain at a 1:500 dilution of the manufacturer’s stock (Invitrogen, S6651). The samples also contained 5 μM NBD protein ...
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bioRxiv - Pathology 2020Quote: ... 1 mM EDTA and 1% SDS) and the protein concentration was quantified by the BCA assay (Thermo Fisher Scientific, #23225). Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
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bioRxiv - Immunology 2024Quote: ... Any bound proteins were eluted by adding a 1:1 mix of 10 mM TBS and LDS buffer (Life Technologies) to the first well and then transferring from well to well ...
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bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in donkey anti-mouse Alexa 647 antibody (1:250, Invitrogen A-31571) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used were 1:500 Alexa flour 488 goat anti-mouse (A11001; Invitrogen), 1:500 Alexa flour 488 goat anti-rabbit (A11008 ...