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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Microbiology 2020Quote: ... were calculated by IFA using primary antibodies against viral proteins and Alexa FluorTM 488 α-rabbit and Alexa FluorTM 586 α-mouse as secondary antibodies (Invitrogen), respectively.
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bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... Mouse lung homogenates were used to determine IgM protein using an IgM uncoated ELISA kit as directed (Invitrogen-ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Pathology 2021Quote: ... lysis of cells and mouse liver tissues was achieved using the Mammalian Protein Extraction Reagent (cat 78501; Thermo Fisher Scientific, Waltham, MA) in association with the Halt Protease Inhibitor Cocktail (cat 78429 ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was immunoprecipitated using 2 μg/ml anti-mouse ST2 antibody and Pierce™ Protein G Magnetic Beads (ThermoFisher, Cat# 88848), followed by western blot analysis.
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (rabbit IgG-, Protein A-, or mouse IgG-conjugated with HRP, Thermo Fisher Scientific) and ECL detection reagent (Amersham) ...
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bioRxiv - Developmental Biology 2024Quote: ... ECs isolated from each mouse were seeded in 2 wells of 6-well plate coated with attachment factor protein 1X (ThermoFisher Scientific, S006100). For a pure EC population ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were prepared as described above and subjected to immunoprecipitation with anti-DYKDDDDK-tag antibody clone 1E6 or mouse IgG anti-HA antibody clone 4B2 using Protein G Dynabeads (Thermo Fisher Scientific). The beads (bound fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin from each sample was then immunoprecipitated overnight with 5μg of the following antibodies (per sample) premixed with Protein G Dynabeads (for mouse antibodies; Thermo Fisher Scientific, 10004D) or Protein A Dynabeads (for rabbit antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were denatured using 1 x NuPAGE® LDS sample buffer (Thermo Fisher Scientific) and reduced with 2-Mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Biochemistry 2020Quote: αT-catenin protein fragments were incubated with or without 1 mM BS3 (Thermo Scientific) in 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation of Cav-1 was performed using Dynabeads™ Protein G Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: THP-1 cells were lysed in M-PER mammalian protein extraction reagent (Thermo Scientific) supplemented with Pierce protease inhibitor (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody is Rabbit polyclonal antibodies against green fluorescent protein (GFP) (1:500, Invitrogen). Secondary antibodies were conjugated with AlexaFluor-488 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl Dynabeads Protein A (Invitrogen 10001D) coated with 10 µg GINS antibodies for 2 h at 4°C with rotation ...
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bioRxiv - Immunology 2022Quote: ... at a protein/trypsin ratio of 1:50 and 10 % acetonitrile (Thermo Fisher Scientific), in a final volume of 100 μl ...
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bioRxiv - Pathology 2024Quote: ... The protein mixture was separated on a 4-12% NuPAGE gel (1 mm, Invitrogen). Samples were run at room temperature for 35 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
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bioRxiv - Genomics 2023Quote: 1 μg of antibody was coupled with 10 μL protein A (Invitrogen, cat# 10002D) and 10 μL protein G (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and Chlamydia heat-shock protein 60 (cHsp60) (ThermoFisher Scientific MA3-023, 1:2000 dilution), followed by secondary antibodies AlexaFluor 488 anti-mouse (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 diluted lysates were quantified using the Pierce BCA protein assay (Thermo Scientific). 30 μg of protein lysates were mixed with equal parts 2x Loading buffer (21% w/v glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... was used to stain proteins and SYTOX™ Deep Red (1 µM, ThermoFisher S11380) was used as a DNA stain.
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 mg of total protein was resuspended in 100 μl NativePAGE sample buffer (Invitrogen), lysed with 1.5% digitonin (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-glial fibrillary acidic protein (GFAP) antibody (Thermofisher, Cat# 13-0300, 1:70), and chicken anti-microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2023Quote: ... All other proteins were detected with secondary antibodies conjugated to DyLight800 (1:10000, ThermoFisher). Images were acquired with a ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used: RhoA (Invitrogen, #PA5-87403, 1:500), Rac1 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: The extracts (1 mg protein) were pre-cleared with control 4%-Agarose resin (ThermoFisher) for 1h at 4 °C with constant rotation ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Glial Fibrillary Acidic Protein (GFAP) Alexa Fluor 488 (Invitrogen, A-21294, 1:200), Ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitated proteins were eluted by adding 1× Bolt LDS Sample Buffer (Thermo Fisher Scientific) mixed with β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and then stained using 1:300 α-mouse-AF488 (Molecular Probes A11001) in 1 % BSA for 30 min at RT in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAb anti-ZO-1 (clone ZO1-1A12) used in immunofluorescence 1:300 on zebrafish embryos was from Invitrogen. Rat mAb anti-HA High Affinity (clone 3F10) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were washed with TBST and further reacted either with alkaline phosphatase-conjugated anti-mouse or anti-rabbit IgG secondary antibody (1:2000) or with horse radish peroxidase-conjugated anti-mouse or anti-rabbit IgG antisera (1:20,000) (Invitrogen) at room temperature for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... (1/2,000) followed by anti-rabbit Alexa 488- and anti-mouse Alexa 594-conjugated IgG (1/2,000, Life technologies). Samples were observed at 100X magnification using a Leica DMi8 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBST and incubated with the correspondent secondary antibody (diluted in 1% BSA in PBST) (goat anti-mouse or anti-rabbit conjugated to AlexaFluor 594 or 488 1:1,000 (Invitrogen)) for 1 hour at RT in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were activated using a 1:1 ratio Dynabead Mouse T Activator CD3/CD28 (#00775477, Thermo Scientific) for 72 hrs in T cell media containing RPMI 1640 (VWR) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... rabbit primary antibody of NPY (at dilution of 1:1,000) and mouse primary antibody of eNOS (at dilution of 1:2,000) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and stained for another 1 hr using 1:1,000 dilution of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen) as a secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... followed by secondary antibody incubation (1 h at room temperature) with fluorescein-labeled anti-mouse IgG (1:200, Invitrogen) in 3% normal goat serum in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... WB: 1:10,000) and goat anti-mouse-HRP (Cat# 31430, WB: 1:10,000) antibodies were purchased from Thermo Fisher. Alexa Fluor 488/594/647 conjugated secondary antibodies for immunofluorescence were purchased from Jasckson ImmunoResearch.
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bioRxiv - Neuroscience 2021Quote: ... but after rinsing sections were incubated in secondary antibodies (donkey anti-guinea pig 555 1:1,000 Sigma SAB4600298; goat anti-mouse 546 1:400 Invitrogen A-11030 ...