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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... HeLa and MCF-7 cells (ATCC) were cultured in the same medium supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher) instead of Nu serum ...
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bioRxiv - Genomics 2021Quote: ... ChIP DNA samples were amplified on an Applied Biosystems real-time PCR machine using site-specific primer sets (Supplementary Table 7) and quantified using SYBR Green (Applied Biosystems) incorporation ...
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bioRxiv - Immunology 2021Quote: ... a T-cell proliferation assay was performed by incubating collected mice spleen cells for 7 min at RT with 5 μM Carboxyfluorescein succinimidyl ester (CFSE) (Life Technologies), staining was quenched with FCS and splenocytes cultured at 106 cells/well in 96-well plates for 5 days at 37°C in 5% CO2 with 3 μg/ml of rSp antigen ...
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bioRxiv - Evolutionary Biology 2021Quote: For ethanol preference assays, experimental substrates were 1% agarose and contained 75mM sucrose and increasing concentrations (3%, 5%, and 7%) of ethanol (ThermoFisher #BP2818); control substrates were 1% agarose and contained 75mM sucrose.
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: Differential scanning fluorimetry protein thermal shift assays were carried out using a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Reactions were pipetted into wells in a MicroAmp FAST optical 96-well plate (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in Alex et al., 2019) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry or electroporation buffer ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction solution was then purified via Zeba spin desalting columns with 7 kDa cutoff to remove unreacted linker (Thermo Fisher). Successful linker conjugation was confirmed using gel electrophoresis and comparison to a size standard of the unmodified Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction solution was then purified via Zeba spin desalting columns with 7 kDa cutoff to remove unreacted linker (Thermo Fisher). Successful linker conjugation was confirmed using gel electrophoresis and comparison to a size standard of the unmodified RBD ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed in technical and biological triplicates in a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Microsoft Excel (v16.54 ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Biophysics 2021Quote: ... 25 mM NaCl and 10 mM β-Mercaptoethanol at pH 7 (pH adjusted with NaOH) was used and 880 units per ml SEC sample RNAseOUT(Invitrogen) as RNAse inhibitor were added to the sample prior to the size exclusion chromatography purification step ...
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bioRxiv - Genetics 2020Quote: ... Barcoding primers were purchased from Eurofins Genomics at 100 μM concentration in HPLC-purified, 96-well plate format. Barcoded amplicons (e.g., Supplementary Fig. 7) were quantified by Qubit fluorometry (Thermo Fisher Scientific), and pooled at equimolar concentrations ...
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were double-blinded microinjected into the cytoplasm of single COS-7 cells (n=100-200 cells per sample) along with fluorescein-labeled dextran (molecular weight 10000, Life Technologies) using a micromanipulator (Narishige) ...
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bioRxiv - Microbiology 2020Quote: ... primers for different viral and cellular transcripts (see Supplementary Table 7 for complete list of primers) and SYBR Green (Thermo Scientific) using the QuantStudio 7 Flex Real-Time PCR System (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... in a 10 µl reaction volume run in triplicate wells of a 96 well MicroAmp plate run on a QuantStudio 7 qPCR cycler (Thermo Fisher) and QuantStudio Real-Time PCR Software v1.3.
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bioRxiv - Immunology 2021Quote: ... immature DCs were generated from bone marrow cells and cultured for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM; Gibco Cat# 12440-053) containing 10% fetal bovine serum Atlanta biologicals Cat# S12450H ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Isolated cells were incubated in sterile petri dishes for 7 to 10 days in BMDM differentiation medium – DMEM (Gibco, 11965-092) supplemented with 30% L-cell conditioned medium ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Immunology 2021Quote: ... BirA enzyme was added at a ratio of 1:200 w/w and incubated for 1 h at 37 °C before being buffer exchanged into PBS [pH 7.4] with a Zeba 7 kDa MWCO spin column (Thermo Fisher Scientific).35
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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NVX-CoV2373 vaccine protects cynomolgus macaque upper and lower airways against SARS-CoV-2 challengebioRxiv - Immunology 2020Quote: ... Reactions were carried out on a Quant Studio 6 and 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts were differentiated on cell culture plastic for 7 days before transfection with two separate siRNAs targeting either MMP8 (105332, 112911; Life Technologies) or MMP9 (104072 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Microbiology 2020Quote: COS-7 cells were seeded onto 6-well plates 24 hours prior to the transient transfection using Lipofectamine 2000 reagent (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 30 minutes with 40 µl/ml CellEvent™ Caspase-3/7 Green ReadyProbes Reagent (#R37111, Life Technologies). Cells were washed with PBS (without Mg2+ and Ca2+) ...
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bioRxiv - Immunology 2022Quote: ... RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems). Samples were normalized by subtracting the respective CT values of housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 10 ng cDNA for each sample was used and all qRT-PCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies) using Brilliant II SYBR Green QPCR Master Mix (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Genomics 2022Quote: ... Duplicates of 10 μL of each sample were transferred into a qPCR plate and measured in a Viia 7 qPCR machine (Applied Biosystems) using following program ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Genomics 2022Quote: ... Slides were then placed in a hybridization chamber, and 75μl of preheated FISH solution (0.9M NaCl, 20mM Tris pH 7 (both Thermo Fisher Scientific, USA), 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Neuroscience 2022Quote: To analyze effector caspase activity in stimulated hippocampal neurons we employed the CellEvent(tm) caspase-3/7 green detection assay (ThermoFisher Scientific) that allows analyzing activity in individual cells ...
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bioRxiv - Immunology 2022Quote: 22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3β-Ol-cholesterol (22-NBD-cholesterol) (N1148) and 7-NBD-PE (N360) were obtained from Thermo Fisher and dissolved in ethanol at 1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2024Quote: ... Gene expression was quantified with an SYBR Green real time-PCR using the ViiA™ 7 system (4453535, Thermo Fisher Scientific). β-Actin was used as a housekeeping gene to normalize the amounts of cDNA in each sample ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...