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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio-7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Bioengineering 2022Quote: ... medium was changed to RPMI + B27-I and on day 7 to RPMI 1640 supplemented with B27 complete supplement (B27C; 17504044, Gibco, USA), which was thereafter used for CM culture ...
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bioRxiv - Cancer Biology 2022Quote: siRNA oligos targeting human lnc152 [described previously (22)] were transfected into MCF-7 cells at a final concentration of 10 nM using Lipofectamine RNAiMAX reagent (Invitrogen, 13778150) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on the Applied Biosystems ViiA 7 using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The level of ACTIN7 (ACT7 ...
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bioRxiv - Bioengineering 2022Quote: ... 7 µm sections were cut on a Leica RM 2135 rotary microtome and mounted on Superfrost Plus microscope slides (Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in (Alex et al., 2019)) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry ...
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bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (SH30243.01, Hyclone GE) supplemented with 10% FBS (10270-106, Gibco-Thermo Fisher Scientific) and 1% penicillin-streptomycin (A001A ...
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bioRxiv - Molecular Biology 2020Quote: ... The sonicated material was then pooled together and distributed into 500 μL aliquots (equivalent of 7*108 cells per aliquot) and 25 μl of Protein G magnetic beads (Life Technologies-Invitrogen) pre-bound with 6μg ...
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bioRxiv - Immunology 2021Quote: ... for 35 minutes at 165V and transferred onto nitrocellulose membranes using the iBlot® 7-Minute Blotting System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... total cellular RNA was extracted from Huh-7 cells treated with oleic acid for 24 hours using TRIZOL reagent (ThermoFisher Scientific) and reverse transcribed using the Superscript VILO (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then resuspended in DMEM + 10% FBS supplemented with 50 μM CellEvent™ Caspase 3/7 Green Detection Reagent (Invitrogen) at 37 °C for 25 min ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qPCR was performed using SYBR Green (BIO-RAD and a ViiA 7 Real Time System (ThermoFisher, Waltham, MA, USA) as specified by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: Huh-7 cells were transfected with the HBsAg or luciferase reporter derived plasmids per manufacturer’s instructions (Lipofectamine 3000, Invitrogen, MA, USA). Cells were treated with the indicated compounds for 5 days ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed using an UltraSYBR Mixture (Beijing CoWin Biotech, Beijing, China) on a ViiA 7 real-time PCR system (Applied Biosystems). Viral rRNAs were normalized to swine 28S rRNA expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Neuroscience 2020Quote: ... PR1/2 and mPRs genes were determined through quantitative PCR (qPCR) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems), and data were analyzed with DataAssist (ABI ...
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bioRxiv - Microbiology 2020Quote: Pseudo-typed viruses were produced as previously described (50), 293T (ATCC, CRL-3216), Huh-7 and Vero (ATCC, CCL-81) cells were maintained in high glucose DMEM (Gibco, USA) supplemented with 10% FBS (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Neurospheres were passaged by centrifuging 5 min at 1,000 rpm and incubating for 7 min in Accutase (Gibco® A11105-01) before mechanically dissociating the spheres into single-cell suspension.
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bioRxiv - Microbiology 2020Quote: ... di- and oligo- saccharides present in the spent media samples were analyzed on a Dionex ICS-5000 HPAEC system operated by the Chromeleon software version 7 (Dionex, Thermo Scientific). Samples were bound to a Dionex CarboPac PA1 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Murine PNPLA2 mRNA levels relative to HPRT transcript levels were measured by the QuantStudio 7 Flex Real-Time PCR System using Taqman® gene expression assays (PNPLA2, Mm00503040_m1; HPRT, Mm00446968_m1, Thermo Fisher Scientific). PNPLA2 relative expression to HPRT was calculated using the comparative ΔΔCt method.27
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Neuroscience 2022Quote: For insulin and rapamycin treatment cells were seeded at 7 × 105 cells per well in a six-well plate (Thermo Scientific) in DMEM + 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using the Fast SYBR Green Master Mix on a QuantStudio 7 Flex Real Time PCR Systems (Applied Biosystems). The sequences of primer pairs (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and Il-1β (in aortic CD3- cells) mRNA levels were assessed by qPCR using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems), and initial differences in RNA quantity were corrected for using the housekeeping genes 36b4 and Cyclophilin B.
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bioRxiv - Cancer Biology 2022Quote: ... Cell number per well by DNA content was determined after 7-10 days of culture using CyQUANT Direct Cell Proliferation Assay (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR was performed for 55-60 cycles for maximal saturation of signal with final extension at 72 °C for 7 min in a 7500 Fast Real-Time PCR System (Applied Biosystems). The primer sequences used were ...
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bioRxiv - Developmental Biology 2019Quote: ... Dead cells were excluded based on forward and side scatter profiles and the uptake of 7AAD (7-Aminoactinomycin D dead cell stain, Life Technologies). Sorted YFP-positive and YFP-negative cells were pelleted by centrifugation and flash frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR amplification was performed using Power SYBR green dye and ViiA 7 Real-Time PCR system (Applied Biosystems, Carlsbad, CA). All primer sets used in the qPCR analysis (Table 3 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cos-7, 293T cells were obtained from The American Type Culture Collection (Manassas, Virginia) and 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR was performed on the QuantStudio 7 Flex Real-Time PCR System using Power SYBR Green PCR Master Mix (Applied Biosystems). The PCR conditions were 950C for 10 min ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... A pre-warmed buffer containing DPBS supplemented with 10% FBS and CellEvent Caspase 3/7 Green (1 μL/1 mL of buffer; ThermoFisher C10427) were added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... for each reaction was incorporated into the standard curve to derive the number of mRNA molecules present using the ViiA 7 software (Applied Biosystems). The standard curve was generated using 13 ...
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bioRxiv - Biophysics 2019Quote: ... The grids were blotted for 4 s with blot force 7 at 85% humidity and 15°C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). Datasets were collected using a Titan Krios operated at an acceleration voltage of 300 keV and the GATAN K2 Summit direct electron detector coupled with the GIF quantum energy filter (Gatan Inc. ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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bioRxiv - Microbiology 2019Quote: ... post-stained in saturated Uranyl acetate in 70% Ethanol (Roth) for 7 minutes and subsequently imaged using an FEI Tecnai F20 electron microscope (Thermofisher, USA).
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA was diluted to 40 μM and was incubated with 7 U/mL DNase I (Thermo Fisher Scientific, Waltham, MA), 10 mM MES pH 5.5 or 10 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... 4 μM glass slide) then for Laser Capture Microdissection (LCM; N=3; 7 μM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522). The slides were stored at −20°C in an airtight container with desiccant until ready for dissection (1 day to 3 months) ...
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bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
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bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
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bioRxiv - Genomics 2020Quote: ... All four RT-PCR reactions were performed for each sample in duplicate using the following cycling conditions on an Applied Biosystems QuantStudio 7 Instrument (Applied Biosystems): reverse transcription at 50 °C for 5 min ...
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bioRxiv - Bioengineering 2020Quote: ... was carried out for 7 and 21 days in a 12-well plate in the nutrient medium containing DMEM medium (Gibco, USA), 10% of fetal bovine serum (Gibco ...
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2019-nCoV RUO Kit (Integrated DNA Technologies) and 2019-nCoV_N positive Control (Integrated DNA Technologies) with a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The redox homeostasis of promastigotes was monitored both qualitatively and quantitively by 2-7 dichlorodihydro fluorescein diacetate (DCFDA) (Life Technologies, USA) staining ...
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bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... University of Nottingham) and Cos-7 (ATCC) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich or Thermo Fisher Scientific) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with an integrity number higher than 7 were used for cDNA synthesis using the Superscript III RT kit (Invitrogen, Germany). Real-time semiquantitative PCR with TaqMan and SYBRGreen were performed in the LightCycler 480 Systems (384-well format ...
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bioRxiv - Neuroscience 2020Quote: ... were collected from FB labelled mice 7-10 days after knee injections in ice cold dissociation media containing L-15 Medium (1X) + GlutaMAX-l (Life Technologies), supplemented with 24 mM NaHCO3 ...