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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 50 mM D+Biotin (Acros Organics)).
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bioRxiv - Cell Biology 2024Quote: ... and oligo d(T)20 (Invitrogen) sequences ...
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Candida albicans enhance Staphylococcus aureus virulence by progressive generation of new phenotypesbioRxiv - Microbiology 2024Quote: ... 2% D-glucose (Fisher Scientific S.L.), and ...
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bioRxiv - Molecular Biology 2019Quote: mRNA was purified from 200 µg total RNA extracted from uninfected and infected Huh7 cells (MOI 1, 48 hours post-infection) using one round of polyA selection (Dynabeads mRNA purification kit; Thermo Fisher) and one round of rRNA depletion (NEBNext rRNA depletion kit ...
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bioRxiv - Neuroscience 2021Quote: Freshly dissected retinas were incubated with gentle agitation for one hour at 37°C in 1 mg/mL of pHrodo Red-conjugated zymosan bioparticles (ThermoFisher Scientific) resuspended in retinal culture media (1:1 mixture of DMEM and F-12 supplemented with L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: ... followed by three washes in 0.1%TritonX-100/PBS and one hour of incubation with the secondary antibodies Alexa Fluor 488 and Alexa Fluor 568 (1:500, Life Technologies). Nuclei were counterstained with DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 2 hours at room temperature in secondary immunoreactive solution containing one or more of the following secondary antibodies: 1:1000 donkey anti-rabbit conjugated to Alexa 488 (Invitrogen A21206) and 1:1000 goat anti-mouse conjugated to Alexa 647 (Invitrogen A21235) ...
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bioRxiv - Immunology 2020Quote: Luciferase-based cytotoxicity assay was carried out for Her2 and CD19 CAR T cells as previously described (22) at a 10:1 ratio of effector to target cells of using Firefly Luc One-Step Glow assay (ThermoFisher #16197). For analysis of cytokine release ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of diluted RNA (1:10) was added to 20μL of Superscript III Platinum One-Step qRT-PCR mix (Invitrogen 11746-100) containing 12.5 μL reaction mix ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 ° C and 1 min at 60 ° C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The standard curve was generated by diluting the DNA of the bacterium Escherichia coli ATCC 25922 in series of 102 – 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 °C and 1 min at 60 °C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The proportion of bacteria in the microbiome was calculated based on the Ct (cycle threshold ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS) and then for one hour in a solution containing phalloidin–Atto647N (1/400 in PBS, Thermo Fisher Scientific, 65906) and DAPI (0.5 μg/ml in PBS ...
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bioRxiv - Immunology 2021Quote: Two different siRNA for thymidylate synthase (TYMS silencer select s14538, s14539) and one control siRNA (silencer select Negative Control #1) (Ambion, Life Technologies) were used at 100 nM ...
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bioRxiv - Genomics 2021Quote: ... We performed one-step multiplex real-time PCR using TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher Scientific, cat. no. A28526), targeting three different SARS-CoV-2 genes with primer and probe sets specific for Spike (S) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Specimens were washed in PBS and incubated for one hour at room temperature using Texas red goat anti-rabbit antibody (1:1000) (Invitrogen, Ontario). Coverslips were adhered using PureLong Gold mounting medium containing DAPI (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... MERS-CoV and REBOV were diluted to 2×10-3 and confirmed by their respective monoplex one-step RT-PCR with specific primers using the Verso 1-step RT-PCR kit (Thermo Scientific). The reaction was set up in 10 μl final volume with 100 nM each of unlabelled forward and reverse primers ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed one final time with PBST and were then developed by adding 1-step Ultra TMB (Fisher Scientific) for 60 seconds ...
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bioRxiv - Developmental Biology 2023Quote: ... one day before the start of the experiment (day -1) cells were cultured in media with exosome depleted FBS (Gibco, 15624559). The day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times with PBS for 20 mins each then incubated for one hour in appropriate fluorescently-conjugated secondary antibodies (1:500, ThermoFisher Australia). Brainstem sections were kept in order and mounted onto gelatin-coated slides ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DNA integrity and purity was monitored on 1% agarose gels and by NanoDropTM One UVVis spectrophotometer (Thermo Fisher Scientific, USA). DNA concentration was further measured using Qubit® 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for one hour at room temperature with blocking buffer containing secondary antibodies for Pax7 (ThermoFisher #A-11072; 1:500) and MyoD (ThermoFisher #A-11001) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse transcribed and amplified in 5 reactions with 1 µg RNA template using SuperScript IV One-Step RT-PCR System with ezDNase (Invitrogen, 12595025) and the primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody incubation was performed for one hour at room temperature: anti-mouse AlexaFluor647 (1:200 dilution, ThermoFisher Scientific #A28181, RRID:AB_2536165). Cells were nuclear stained with Hoechst 33342 (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Specificity and concentration of purified PCR products were checked on 1% agarose gel and with a NanoDrop One© spectrophotometer (ThermoFisher), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated RNA (1 μg per sample) was used to program cDNA synthesis (SuperScript™ III One-Step RT kit, 12574-018 Invitrogen). Expression of pluripotency markers and determination of tri-lineage differentiation potential was evaluated using the TaqMan hPSC Scorecard™ Panel (A15876 ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were incubated for 1 h at RT with one of the following secondary antibodies: goat anti-rabbit Alexa Fluor® 488 (1:500, A-11008, Invitrogen) was used for TDP-43 ...
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots were solubilized in 1 mL 8M urea lysis buffer: 8M Urea (Invitrogen, CA; CAS# 57-13-6), 100mM NaCl(Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Microbiology 2019Quote: ... Mice were euthanized by CO2 asphyxiation at 6 h and lungs placed in 1 ml TRIzol (Thermo Fisher). RNA was then extracted using acid-phenol-chloroform-isoamyl alcohol (125:21:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded in 6-well plates in 1 ml of RPMI 1640 medium (Gibco, Amarillo, TX, USA) supplemented with 10% fetal bovine serum and 0.2% primocin (Invivogen ...
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bioRxiv - Molecular Biology 2021Quote: ... HL-1 cells in 6-well plates were transfected with 15 µL Lipofectamine 2000 reagent (Life Technologies 11668019) complexes with nucleic acid ...
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bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... and selected antibiotics according to the respective expression constructs: blasticidin S (6 μg mL−1, Thermo Fisher Scientific), puromycin (0.5 μg mL−1 ...
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bioRxiv - Immunology 2022Quote: ... rested for 6 hours and stimulated overnight in 1 ml of complete medium (RPMI 1640 with Glutamax, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Microbiology 2024Quote: ... Beads were washed 6 times with 1 mL each of ChIP buffer on a magnetic tube rack (Invitrogen). After removing the final wash ...
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bioRxiv - Genomics 2023Quote: ... Cells were then co-transfected with the two plasmids at a molar ratio of 6:1 (2.5 μg of homology vector, ∼0.7 μg of sgRNA vector) using Lipofectamine2000 (ThermoFisher). The next day ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1, Thermo Fisher Scientific, A1113903) and hygromycin B (0.3 mg mL-1 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated in primary antibody (chicken anti-GFP, Aves labs GFP-1010, 1/500; goat anti-PDGFRA, R&D Systems AF1062, 1/200; rat anti-SOX2, ThermoFisher 14-9811-82 ...