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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1 mM dithiothreitol, 1% BSA, 0.1% Tween 20, and 0.6 U/mL RiboLock [Thermo Fisher]). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 1×3–5 min with 1x PBS + 1 ng/mL DAPI and mounted in ProLong Gold antifade reagent (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... containing 3 mL of home-made Expansion Medium consisting of a 1:1 mixture of DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 1 µL mouse anti-V5 primary antibody (1:10,000; R96025, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and combined with 50μl 1× D-Luciferin Working Solution supplemented with 1× Firefly Signal Enhancer (Thermo Scientific Pierce Firefly Signal Enhancer; Thermo Scientific 16180). Following a 10 min dark incubation period to allow for signal stabilization ...
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bioRxiv - Molecular Biology 2021Quote: ... and shINTS11 cells were seeded at a density of 1 × 106 cells in 10 cm dishes at d-1 in DMEM medium (Gibco, #11965-084), supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cell Biology 2020Quote: Naïve mouse ESCs and D-EPSCs were cultured in a base medium of N2B27 prepared as follows: 1:1 ratio of DMEM/F12 (ThermoFisher Scientific 21331020) and Neurobasal (ThermoFisher Scientific 21103049) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-APP (22C11, 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Genetics 2023Quote: ... 4 mM 4-acetamido-4’- maleimidylstilbene-2,2’-disulfonate (AMS) (Invitrogen, catalogue no. A485), and then keep in the dark at room temperature for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted total RNA from 3-4 biological replicates per sex and line with a PureLink RNA purification kit (Thermo Fisher Scientific, USA) and validated the samples with the Bioanalyzer RNA 6000 Nano kit (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
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bioRxiv - Molecular Biology 2023Quote: ... and two solid-phase extraction columns (150 µm × 3 cm) packed with MAbPac particles (C8 resin, 4 µm diameter, 300-Å pore size; Thermo Fisher Scientific) were used ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...
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bioRxiv - Bioengineering 2020Quote: ... the ADSC and the HUVEC were added after trypsin detachment (both always used at passages 1-6) and centrifugation 1 minute at 3,500 rpm (Minispin, ThermoFisher Scientific, Whaltam, MA, USA) to get a final cell concentration of 4×106 ADSC/mL and 2×106 HUVEC/mL ...
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bioRxiv - Plant Biology 2021Quote: ... The liquid syringe was washed 6 times with each of 10 µL of 1:1 (v/v) acetone:hexane and HPLC grade methanol (Fisher Scientific Co, Edmonton Alberta) both before and after the liquid injection.
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bioRxiv - Developmental Biology 2020Quote: ... antibodies were diluted 1:500 in PBT with 1% DMSO and 1% BSA and applied together with TO-PRO-3 (T3605, Thermo Fisher, Waltham, MA) diluted 1:1000 over- night at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...
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bioRxiv - Immunology 2021Quote: ... 5/6 weeks and 6/7 weeks post-infection by flow cytometry using counting beads (CountBright, ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... transferred to 6 ml liquid MSgg medium of each well of a 6-well microplate (Thermo Scientific), and then grown for additional four days ...
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bioRxiv - Microbiology 2020Quote: ... 6 × 105 293LTV cells were seeded in a 6-well plate and transfected using Lipofectamine 2000 (Invitrogen) which was complexed with DNA plasmids driving the expression of either VSV G protein (positive control) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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bioRxiv - Cell Biology 2023Quote: ... Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen). Constructs were bombarded into unc-119(ed3 ...
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Microbiology 2019Quote: ... in which one uninfected male and one uninfected female were introduced in an arena (Petri Dishes 100 x 150 mm, Fisher Scientific, Ontario, Canada). For the second treatment ...
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bioRxiv - Microbiology 2023Quote: ... were used as template in a one-step RT-PCR reaction with the SuperScrip IV One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) and primers that amplify coding sequences for Spike amino acids 614 and 655 (Table 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... and the RNA concentration was measured by UV spectroscopy (NanoDrop One Microvolume UV-Vis Spectrophotometer, ND-ONE-W, Thermo Fisher Scientific, Waltham, MA). Reverse transcription was performed using the iScript cDNA Synthesis Kit (1708891 ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured in complete DMEM (4.5 g/L D-Glucose, L-Glutamine, 1% penicillin/streptomycin, Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μM D-luciferin) for 1 hour then cycloheximide (CHX) (Fisher Scientific, Hampton, NH, cat # 50255724) was added at a final concentration of 100μg/ml diluted in serum free media.
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by the addition of 1 mM isopropyl β-D-thiogalactoside (IPTG) (Thermo Scientific) for 16 h at 22°C ...