Labshake search
Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
-
bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
-
bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with the following secondary antibodies for 3 h at room temperature: anti-rabbit IgG-Alexa488 and anti-rat IgG-Alexa488 (1:1000 for both; Invitrogen Co). Sections were mounted on microscope slides and embedded with Vectashield that includes DAPI for nuclear staining (Vector Laboratories ...
-
bioRxiv - Neuroscience 2020Quote: ... The sections were then washed and incubated for 60 min at room temperature with an appropriate secondary antibody: anti-rat (1:500, Thermo Fisher Scientific Cat# A-11006 ...
-
bioRxiv - Pathology 2020Quote: ... cryoslides were incubated for 2 hours at room temperature with secondary antibodies (AlexaFluor 488-, 555-, 647-, or Cy3-conjugated anti-rabbit, rat, mouse, goat IgG) (1:400, Life Technologies, Waltham ...
-
bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2021Quote: ... Alex Fluor 546 donkey anti-rabbit secondary antibodies and Alex Fluor 647 goat anti-rat secondary antibodies (1:500; Molecular Probes) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibody incubation was performed for 30-60 minutes in room temperature with anti-rat Alexa 555 (1:400, Invitrogen, A21434). Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked for 30 minutes using 5% skimmed milk in PBST and then probed with rat anti-GFAP primary antibody (1:2000, cat # 13-0300, Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-rat IgG (catalog #62-9520) and Zysorbin-G (catalog #10-1051-1) were from Invitrogen (Carlsbad, CA). The mouse IgG1 (catalog #401407) ...
-
bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
-
bioRxiv - Cancer Biology 2024Quote: ... The following secondary antibodies and dilutions were used: goat anti-rat Alexa Fluor 488 1:1000 (Thermo Fisher Scientific, A-11006), goat anti-rat Alexa Fluor 594 1:1000 (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... secondary Alexa Fluor 488 antibodies (diluted 1:200 in 0.1% BSA in PBS, goat Anti-Rat-IgM, Thermo Fisher Scientific, Denmark) were added and the sections were incubated overnight at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by the secondary antibody Alexa Fluor 647 goat anti-rat (1:500, Cat# A-21247; Thermo Fisher Scientific, RRID: AB_141778). Later ...
-
bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 45 min at R.T ...
-
bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 1 h at R.T.
-
bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 568 and anti-mouse Alexa Fluor 488 (1/25, A11077 and A11001, ThermoFisher Scientific) for 45 min at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... including anti-rabbit or anti-mouse Alexa Fluor 568 and anti-rat Alexa Fluor 488 (all 1:1000, Thermo Fisher Scientific), were added and incubated for 2 h at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
-
bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
-
bioRxiv - Bioengineering 2024Quote: ... and then stained for 1 h at room temperature in a humidified chamber with AlexaFluor-488-labeled donkey polyclonal anti-rat IgG (1:500, Invitrogen, A21208) and AlexaFluor-555-labeled donkey polyclonal anti-rabbit IgG (1:500 ...
-
bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used were conjugated Alexa Fluor 488 or 647 against rat or rabbit (ThermoFisher, A11008, A11006, A21245, A21247; 1:500) and conjugated Alexa Fluor 488 against chicken (Abcam ...
-
bioRxiv - Genomics 2024Quote: ... and incubated with either anti-rabbit or anti-rat secondary antibodies with AF594 (diluted 1:400, A11012 or A1107, Thermofisher Scientific) for 1 hour ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 1h at RT and stained o/n with antibody fluorescent conjugates (1:500, anti-mouse-AF488, anti-rabbit-AF555 or 647, anti-rat- AF546, all from Thermo-Fisher Scientific), 1mg/ml DAPI solution 1:500 and PNA-lectin AF594 1:1000 (1mg/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 565 (1:500, anti-rat coupled to Alexa Fluor 546, Cat No. A11081, Fisher Scientific, Waltham, MA, USA) secondary antibodies diluted in blocking solution ...
-
bioRxiv - Plant Biology 2024Quote: ... and horseradish peroxidase-labeled goat anti-rat as the secondary antibody at a dilution of 1:5000 (Invitrogen, catalog no. 31470).
-
bioRxiv - Pathology 2024Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (Invitrogen A-11006, 1:500) for one hour ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
-
bioRxiv - Genomics 2023Quote: ... followed by overnight incubation at 4 °C in a primary antibody cocktail (Rabbit anti-NKX2.1, Abcam ab76013 at 1:200; Rat anti-SOX2, Thermo Fisher Scientific 14-9811-80 at 1:100). After washes with TBS-T ...
-
bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
-
bioRxiv - Biophysics 2021Quote: ... A stable HeLa Kyoto cell line expressing mEOS3.2-alpha tubulin and CENPA - GFP was generated and selected using puromycin and blasticidin (ThermoFisher) (Yu et al ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cell lines were cultured and maintained in MEM-alpha and RPMI 1640 containing 10% FBS (Gibco, Life Technologies) incubated at 37°C supplied with 5% CO2 ...
-
bioRxiv - Cell Biology 2020Quote: ... The freshly isolated mesenchymal stem cells were divided into two experimental groups respectively cultured in either Alpha-MEM (Gibco) supplemented with 20% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cell lines were cultured and maintained in MEM-alpha and RPMI 1640 containing 10% FBS (Gibco, Life Technologies) incubated at 37°C supplied with 5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: Cytokines released by primary microglia in the culture medium were quantified by ELISA using the following commercial kits according to manufacturer’s instruction: Mouse TNF alpha Uncoated ELISA kit (88-7324-22, Invitrogen), Rat IL-6 DuoSet ELISA (DY506 ...
-
bioRxiv - Neuroscience 2021Quote: MyoD hiPS cells are seeded on 5µg/ml laminin-521-coated (Biolamina) in 5% KSR medium composed of Alpha-MEM (12571-063, Gibco), 5% KSR (10828028 ...
-
bioRxiv - Genomics 2021Quote: P19 embryonal carcinoma (EC) cells were maintained in the proliferation medium (minimum essential medium-alpha (MEMα; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... Human alpha thrombin (Nordic Diagnostica AB, Billdal, Sweden) was resuspended in OptiMEM reduced serum medium (RSM) (Thermo Fisher Scientific) to 1 U/μl and stored at −20 °C ...
-
bioRxiv - Developmental Biology 2022Quote: ... ovaries were harvested and placed in HEPES-buffered alpha minimum essential medium (αMEM; Gibco, Life Technologies, New York, USA) supplemented with 3 mg/mL BSA (MP Biomedicals ...
-
bioRxiv - Developmental Biology 2022Quote: ... ovaries were harvested and placed in HEPES-buffered alpha minimum essential medium (αMEM; Gibco, Life Technologies, New York, USA) supplemented with 3 mg/mL BSA (MP Biomedicals ...
-
bioRxiv - Neuroscience 2022Quote: 2 mL screw cap tubes with conical bottom (Alpha Laboratories; CP5932) were filled with ribolysing beads (Fisher Scientific; 15515809) to cover the bevelled bottom of the tube and weighted ...
-
bioRxiv - Immunology 2024Quote: ... and allowed to incubate with a combination of Alexa Fluor 488-labeled anti-alpha smooth muscle actin antibody (Invitrogen/Thermo product # ...
-
bioRxiv - Developmental Biology 2023Quote: ... and the cell pellet was resuspended in Alpha Minimum Essential Medium (α-MEM; GIBCO BRL, Grand Island, NY, USA) supplemented with 15% (v/v ...
-
bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... the interscutularis muscle was exposed and labeled with 5 µg/ml Alexa 647-conjugated alpha-bungarotoxin (ThermoFisher Scientific, USA) for 10 minutes at room temperature to stain acetylcholine receptors as a guide for neuromuscular junctions ...