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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... QPCR was performed using a StepOnePlus Real-Time PCR System (Applied Biosystems). Each 20 μL reaction contained 2x TAQMAN Fast Advanced Master Mix (10 μL) ...
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bioRxiv - Molecular Biology 2024Quote: ... analysed with the QuantStudio 7 Flex Real-Time PCR System (Thermo Scientific). The primers were designed using Primer BLAST65 and are listed in Supplementary Table 5.
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bioRxiv - Immunology 2024Quote: ... qPCR was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems). Abundance of SARS-CoV-2 N mRNA was normalized to that of GAPDH ...
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bioRxiv - Plant Biology 2024Quote: ... Reactions were placed in a Quantstudio3 Real-Time PCR system (Applied Biosystems) using the following PCR cycle ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed using the QuantStudio 6 Flex detection system (Applied Biosystems) and SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... was used for RT-PCR amplification and detection was performed using ViiA7 (Life Technologies) or Step One Plus Real-Time PCR system(Applied Biolsystems) ...
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bioRxiv - Cell Biology 2019Quote: ... The resulted cDNA was subjected to quantitative real-time PCR (qRT-PCR) using the DyNAmo HS SYBR Green qPCR Kits (Thermo Fisher Scientific) and the Bio-Rad real-time PCR system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription and PCR amplification were performed using TaqMan RNA-to-CT 1-Step Kit and the StepOnePlus™ real-time PCR instrument (Applied Biosystems). The following probes were used in this study ...
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bioRxiv - Microbiology 2021Quote: ... All reactions were performed on an Applied Biosystems 7300 Real-Time PCR System using the Power SYBR Green PCR Master Mix kit (Applied Biosystems, France), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed by using SYBR Green PCR master mix kit in a stepOnePlus Real Time PCR system (Applied Biosystem by Life Technologies, USA). To validate the gene expression profiles identified by RNA-Seq ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from mouse lung tissue using Trizol with analysis of 25 ng of RNA reverse transcribed and complementary DNA was amplified by real time-PCR using a One-step VERSO SYBR green PCR kit (Thermo Fisher Scientific) on a BioRad CFX96 Real Time PCR machine ...
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bioRxiv - Microbiology 2020Quote: ... A quantitative real-time reverse transcription-PCR (qRT-PCR) reactions were performed using the PowerUp SYBG Green Master Mix Kit (Applied Biosystems, USA), in which samples were processed in duplicate using the following cycling protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real time PCR reactions were done in ABI PRISM™ 7500 Real-Time PCR system using Power SYBR Green PCR Master Mix kit (Applied Biosystems), with three replicates per RNA sample ...
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bioRxiv - Physiology 2020Quote: Quantitative RT-PCR The relative abundance of target gene mRNA in the total RNA extracts was estimated by quantitative reverse transcription (RT) real-time PCR (StepOnePlus, Applied Biosystems, Carlsbad, CA, USA). Real-time PCR reactions were performed using the Power SYBR Green PCR Master Mix Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed with qPCRBIO SyGreen Mix LoRox polymerase (Cultek) in a 7500 Fast Real-Time PCR System (Applied Biosystems by Life Technologies). Thermal cycling was carried out according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... The RT-qPCR was carried out on a QuantStudio 3 Real-Time PCR Instrument using PowerUp SYBR Green Master Mix (Thermo Fisher, cat. no. A25742). The following primers were used ...
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bioRxiv - Plant Biology 2022Quote: Total RNA for real-time RT-PCR was isolated from rosette leaves of 25-day-old plants using Trizol® reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:10 for RT-qPCR measurements using PowerUp SYBR Green Master Mix (A25742, ThermoFisher. QuantStudio 5 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) was used for amplification and fluorescence detection ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of target genes was analyzed via Quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific).
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bioRxiv - Physiology 2024Quote: ... The template cDNA was diluted 5-fold and used for RT-qPCR using a StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) that ran for 40 cycles ...
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bioRxiv - Microbiology 2023Quote: Real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) assays were performed on an ABI 7500 Fast real-time PCR machine (Applied Biosystems, Waltham, MA, USA) in a total volume of 20 µl ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was carried out using Sybr Green Master Mix (A&A Biotechnology) and QuantStudio Real-Time PCR System (Applied Biosystems). Gene expression was normalized to elongation factor-2 (EF2) ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was carried out using Sybr Green Master Mix (A&A Biotechnology) and QuantStudio Real-Time PCR System (Applied Biosystems). Gene expression was normalized to elongation factor-2 (EF2) ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using custom TaqMan Gene Expression Assay reagent on QuantStudio 12K Flex Real-Time PCR System (Thermo Scientific).
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR assays were performed in 20 μL reaction volumes using a QuantStudio 6 flex Real-time PCR system (Applied Biosystems). The primers used are listed in Supplemental Table S1.
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems, ThermoFisher Scientific). qPCR was performed using SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems, ThermoFisher Scientific). qPCR was performed using SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... reverse transcription and qPCR were performed using the Ambion PowerSYBR Green Cells-to-CT kit following manufacturer’s instructions in a 7500 FAST Real-Time PCR thermocycler with v2.0.5 software (Applied Biosystems). The mRNA expression level of each gene relative to GAPDH was calculated using the ΔΔCt method ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... QPCR was performed using a real-time PCR kit provided with a Fast-SYBR Green fluorophore (Applied Biosystems) with 200 nM of each primer in order to keep PCR efficiency between 80% and 100% for all target shbg genes ...
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bioRxiv - Microbiology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and 7500 Fast Real-Time PCR System (Applied Biosystems™) was used ...
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bioRxiv - Microbiology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and 7500 Fast Real-Time PCR System (Applied Biosystems™) as previously described 42 ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative qPCR was performed with Power SYBR® Green PCR Master Mix kit (Life Technologies, UK) using the StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Biophysics 2022Quote: ... The real-time PCR reactions were performed with the TaqMan RNA-to-CT 1-Step Kit (Applied Biosystems) by preparing samples as follows (total 10 μl reaction) ...
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bioRxiv - Developmental Biology 2022Quote: ... and cDNA was subjected to real time PCR using the StepOnePlus system and SYBR Green Kit (Applied Biosystems). Gapdh was used as an internal control and expression differences were determined using the -ΔCT method ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using SYBR Select PCR master mix on a ViiA7 Real-Time PCR System (ThermoFisher). Fold changes were calculated by the 2−(ΔΔCt ...
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bioRxiv - Molecular Biology 2020Quote: ... SuperScript IV One-step RT-PCR kit (ThermoFisher) was used for detection of transcripts ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific, Waltham, USA). The relative gene expression was identified using 2-ΔΔCt value (72 ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Pathology 2024Quote: ... The pulp tissues were transfected with mirVana miRNA mimic miR-27a-5p or mirVana miRNA mimic Negative Control #1 (Thermo Fisher Scientific) using Lipofectamine RNAiMAX ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg/kg body weight miR-182-5p-mimic or negative control was injected via the tail vein using Invivofectamine® 3.0 (Invitrogen, Carlsbad, US). Mice were phenotypically characterized by NMR on days −1 and 7 and a glucose tolerance test after 6 h fasting on day 5 ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript III One-Step RT-PCR Kit with Platinum Taq (Invitrogen, USA) and the following cycling conditions ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript III One-Step RT-PCR Kit with Platinum Taq (Invitrogen, USA) according to the manufacturers’ recommendations ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using the SuperScript® IV One-Step RT-PCR kit (Thermo Fisher Scientific). Nine cDNA fragments (gF1 to gF9 ...
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bioRxiv - Microbiology 2020Quote: Semi-quantitative PCR was carried out on a 7500 Fast Real-time PCR system (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR was performed using StepOne Real-Time PCR System and Taqman Gene Expression Assays (Applied Biosystems) using standard settings (2′ 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR assays were run on a 7500 Fast Real Time PCR system (Thermo Fisher, USA) and the data were processed using 7500 software v2.3 ...
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bioRxiv - Microbiology 2019Quote: ... The qRT-PCR reactions were performed in a Step One real time PCR system (Applied Biosystems) using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Real-time quantitative PCR for mRNA was conducted with SYBR Green PCR master mix (Applied Biosystems) and performed on an ABI 7900HT (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... cDNA was then quantified utilizing Taqman Q-PCR probes (QuantStudio 5 Real-Time PCR System, ThermoFisher). Data was then analyzed using Microsoft Excel and plotted in GraphPad PRISM.
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bioRxiv - Plant Biology 2022Quote: ... The PCR program was set on a StepOne™ Real-Time PCR Machine (Applied Biosystems, UK) as follows ...