Labshake search
Citations for Thermo Fisher :
3101 - 3150 of 6821 citations for Recombinant Human IL1RL1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: 20µg of rhEPO and reference product Eprex® were treated with 20 units of recombinant PNGase F (Thermo Fisher Scientific, USA) at 37 ºC for hours ...
-
bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
-
bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
-
bioRxiv - Cancer Biology 2023Quote: ... LN single cell suspension were stimulated for 5h at 37°C with 5ug/ml of LCMV-GP61-80 in the presence of 50 U/ml recombinant murine IL2 (Thermofisher P2747) and 1mg/ml Brefeldin A (Sigma) ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
-
bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
-
bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by staining with secondary anti-Rabbit antibodies (1:500) conjugated to Alexa Fluor 555 (Goat anti-Rabbit IgG, Superclonal Recombinant Secondary Antibody, Alexa Fluor 555, Thermofisher, # A27039) for 30 minutes on a shaker at Room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
-
bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
-
bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
-
bioRxiv - Microbiology 2020Quote: Toxicity of the surfaces was tested on human immortalized HaCaT keratinocytes (ThermoFisher). Cells were maintained in DMEM high glucose medium supplemented with Na-pyruvate (1mM) ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... 293T and human secondary fibroblasts cells were cultured in DMEM (Gibco, 11995) supplemented with 10% heat-inactivated FBS (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... in tissue culture plates coated with 2.5μg/cm2 human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (Omega Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... HCT116 cells (human colorectal epithelial) were maintained using DMEM (Gibco, 41966-029) supplemented with 10% FBS (Sigma ...
-
bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... Human iPS WT-11 cells were cultured on Vitronectin (Thermo Fisher Scientific) coated 6-well plates or glass coverslips (for smRNA FISH purposes ...
-
bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then incubated in goat anti-human HRP secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution in blocking buffer for 45-60 min at RT ...
-
bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
-
bioRxiv - Biochemistry 2022Quote: ... 100 units/ml human LIF supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in a humidified incubator at 37°C and 5% CO2.
-
bioRxiv - Developmental Biology 2022Quote: ... Labelled DNA was then hybridized to Clariom S human GeneChips (ThermoFisher Scientific) and incubated at 45℃ in the GeneChip Hybridization oven 640 (Affymetrix ...
-
bioRxiv - Genomics 2019Quote: ... The labeled DNA was then hybridized to Human Gene ST1.0 microarrays (Affymetrix), washed ...
-
bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
-
bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc. ...
-
bioRxiv - Cancer Biology 2019Quote: ... anti-human-Alexa 488 or –Alexa 555 (Invitrogen cat #A11013 and #A21433) were used ...
-
bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...
-
bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...
-
bioRxiv - Immunology 2020Quote: ... Cross-adsorbed goat anti-human IgG (H+L) secondary antibody (A18811, Invitrogen) was used at a dilution of 1:3,000.
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco) 7 days prior to nucleofection ...
-
bioRxiv - Molecular Biology 2019Quote: ... All immortalized human myoblasts were cultured in F10 medium (Gibco/ThermoFisher Scientific) supplemented with 20% FBS (GE Healthcare Life Sciences ...
-
bioRxiv - Molecular Biology 2019Quote: ... All immortalized human myoblasts were cultured in F10 medium (Gibco/ThermoFisher Scientific) supplemented with 20% FBS (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2019Quote: ... Human insulin-like growth factor 1 (IGF-1) was from Thermo Fisher and used as IGF-1 receptor activator.
-
bioRxiv - Cell Biology 2019Quote: ... Human TERT-RPE cells were cultured in DMEM+F12 (1:1) (Invitrogen) containing 10% FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... activated using Dynabeads Human T-Activator CD3/CD28 beads (Gibco, Life Technologies), and cultured in X-VIVO 15 (Lonza Cat# BE02-053Q ...
-
bioRxiv - Bioengineering 2019Quote: ... activated using Dynabeads Human T-Activator CD3/CD28 beads (Gibco, Life Technologies), and cultured in X-VIVO 15 (Lonza Cat# BE02-053Q ...
-
bioRxiv - Biochemistry 2021Quote: ... Human embryonic kidney 293T cells (HEK-293T) were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
-
bioRxiv - Genetics 2020Quote: ... and quantified using the Quantifiler Human DNA Quantification kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 goat anti-human IgG (H+L) (Molecular Probes, A21445) (1:500 IF) ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were activated using Dynabeads Human T-Activator CD3/CD28 beads (Invitrogen) according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech ...
-
bioRxiv - Bioengineering 2021Quote: ... RBCs were incubated with anti-Human IgG Alexa Fluor 488 (Thermo Fisher) for 2 h ...
-
bioRxiv - Bioengineering 2021Quote: Human-derived cell lines (HEK293FT and HeLa) were purchased from Invitrogen (R70007) and American Type Culture Collection (CCL-2) ...
-
bioRxiv - Biochemistry 2020Quote: Human ASCT2 (hASCT2) was produced in Pichia pastoris X-33 strain (Invitrogen) in fermentor59 and purified using DDM and CHS (Anatrace ...
-
bioRxiv - Biophysics 2021Quote: ... while HRP-conjugated anti-human IgG Fc (Invitrogen™, Waltham, MA, USA) diluted 1:10,000 in PBS was used in wells treated with REGN10987 ...