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Citations for Thermo Fisher :
3351 - 3400 of 6821 citations for Recombinant Human IL1RL1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Biochemistry 2022Quote: ... CFTR expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Synthego, Redwood City, CA, USA), recombinant 2NLS-Cas9 nuclease (20 μM, Synthego, Redwood City, CA, USA) and optiMEM (Invitrogen, Waltham, MA, USA) supplemented with Y27632 (10 μM) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Microbiology 2023Quote: ... The sequences of all four recombinant viruses generated in this study were confirmed by sequencing with a SeqStudio Genetic Analyzer (Thermo Fisher Scientific) and an outsourced service (Fasmac).
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were centrifuged at 1400 rpm for 4 minutes and incubated with Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (ThermoFisher, MA5-14520) at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2024Quote: ... and 33 µl of this buffer was added to the beads together with 10 µl Recombinant Proteinase K Solution (20 mg/ml, Thermo Fisher, AM2548) and 3 µl RiboLock RNase inhibitor (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
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bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher). Templating and sequencing were performed with the Ion PI HiQ OT200 kit ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Perforin eF450 (1:100, clone dG9, Invitrogen, cat # 48-9994-42). Cells were analyzed on a BD LSR Fortessa flow cytometer ...
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bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
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bioRxiv - Immunology 2021Quote: After 48 h of stimulation with Human T-activator CD3/CD28 Dynabeads (ThermoFisher) CD4+ lymphoblasts were recovered for transfection of CRISPR/Cas9 nucleoprotein complexes ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and 10 µl/ml Fibronectin from human plasma (Thermo Fisher Scientific, #33016-015)] for 2-3 h at 37 °C/5% CO2 and stored at 4 °C after thorough removal of coating solution.
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bioRxiv - Physiology 2019Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: We used GeneChip Human Exon 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) to measure the expression levels of transcripts in each brain tissue specimen ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse and human PSCs were cultured in DMEM (10-013-CV; Fisher Scientific) containing 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit, Thermo Scientific, USA) in plasma.
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bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...