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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Neuroscience 2023Quote: ... samples were protected from light going forward and incubated for 5 days at 37 °C in 1.6 ml of PTwH + 3% donkey serum containing a 1:250 dilution of secondary antibody (donkey anti-Rabbit Alexa 647, ThermoFisher Scientific). Samples were washed 5x 5 min and incubated overnight at rt in 2 mL of PTwH.
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed in PBS with 0.3% Triton X-100 (3 × 10 min) and then incubated with donkey-anti-guinea pig 647 (1:1000; ThermoFisher Cat# A21450) and donkey-anti-rabbit 488 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Stimulations of individual neuromasts consisted in trials of 3 consecutive 1 s-long pressure-application of either control – aCSF alone with 10 µM Alexa 594 (Invitrogen, A10438) - or drug – aCSF with 10 µM Alexa 594 completed with 1 mM serotonin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Quantitative real-time PCR reactions to amplify 1 ng of total cDNA were performed in a QuantStudioTM 3 Real-Time PCR Cycler (A28132, Applied Biosystems) using the Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and slides were washed 3 x 5min in PBS before the application of 1:500 goat α-rabbit AlexaFluor488 antibody (ThermoFisher) for 30min ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed 2 × 5 minutes in 0.1 M PBS and incubated for 3 hours in a solution containing donkey anti-rabbit conjugated to Alexa Fluor 647 (1/500 dilution; Invitrogen, UK). After secondary antibody incubation ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 1% Matrigel and 3 µM CHIR in a 24-well plate with a super-low cell attachment surface (174930, Thermo Scientific) on an in-incubator orbital shaker ...
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bioRxiv - Neuroscience 2024Quote: ... 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 3-week-old plants treated with 1 mM SA or H2O using Trizol39 (Thermo Fisher Scientific). DNase-treated total RNA was then used for SuperScriptIII Reverse Transcription (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products and the vector were mixed at a 1:3 molar ratio and ligated using T4 DNA ligase (Thermo Scientific). For HiFi cloning ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Immunology 2024Quote: ... at a 3:1 bead-to-cell ratio in T-cell media consisting of OpTmizer CTS SFM media (Thermo Fisher Scientific) supplemented with 5% human AB serum and 100 Units/mL human IL-2 (PeproTech) ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... IDT cat. # 10006776) and Promega® GoTaq® 1 Step RT PCR (Cat. # A6020) in a QuantStudio 3 thermocycler (Applied Biosystems) and following the recommendations of the CDC (https://www.cdc.gov/coronavirus/2019-ncov/lab/virus-requests.html) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in TBST for 10 minutes 3 times and incubated in Alexa Fluor conjugated secondary antibodies diluted 1:200 (Life Technologies) with DAPI (1:50,000 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature for 3 h in TBS containing AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were checked for 60-70% confluency and infected with 1:3 dilution of the pseudoviral stock in Opti-MEM (ThermoFisher: 31985062). Cells were incubated in low serum condition for 24 hours at 37°C before both luciferase and flow cytometry assays.
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bioRxiv - Microbiology 2023Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (0.5 μg/cm2) and Lipofectamine 2000 (1 μg RNA:3 μL reagent) were added separately to 50 μL opti-MEM (Thermo Fisher) and incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA was synthesized in 20 μl reactions with 1-3 μg of RNA using 200 ng of random hexamer primers and 200 U Superscript III reverse transcriptase (Thermo Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were then washed 3 times with 1×PBS and stained with 0.5 µg/ml DAPI (Thermo Fisher Scientific, cat# D1306) in 1×PBS for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were washed 3 x in PBS and labeled with donkey anti-mouse AlexaFluor 647 (1:1000, A31571, Thermo Fisher), for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... On day 5 the media was replaced with PC/SMC2 media for 24 hours and then cells were passaged 1:3 on to fibronectin (FC01010MG, Fisher Scientific) coated tissue culture plates in PC3 media ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then transfected with the gRNA library and NLS-Bxb1 recombinase plasmids at a ratio of 3:1 (w/w) using a FreeStyle Max transfection reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Movies were recorded at a magnification yielding 0.84 Å/pixel with a dose rate of 16.3 e-/Å2/s and a defocus range between -1 to -3 μm using the Smart EPU Software (Thermo Fisher Scientific). Movies were built of 50 frames each with a total dose of 50.8 e−/Å2 (∼1 e−/Å2 per frame ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were rinsed 3 x 1hr in 1XPBS and incubated with secondary antibody (AlexaFluor Plus 594 highly cross absorbed, 1:1000 (Molecular Probes)) for 1 hr at room temperature and then moved to 4°C for incubation overnight ...
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bioRxiv - Cell Biology 2023Quote: ... biopsies were co-cultured with 3T3-J2 fibroblasts and Rho-associated protein kinase inhibitor (Y-27632) in epithelial cell expansion medium consisting of a 3:1 ratio DMEM:F12 (Gibco, UK; 21765), 1X penicillin/streptomycin and 5% FBS (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Slides were again washed 3 times with 50 mL of PBS and incubated with anti-mouse IgG-Alexa 488 secondary antibody (1:1000; ThermoFisher A28175) at 37°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for rabbit GFP 1:500 (Invitrogen, A11122) for one hour at room temperature with shaking.
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bioRxiv - Microbiology 2023Quote: ... On day 5 the media was replaced with PC/SMC2 media for 24 hours and then cells were passaged 1:3 on to fibronectin (FC01010MG, Fisher Scientific) coated tissue culture plates in SMC3 media ...
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bioRxiv - Plant Biology 2023Quote: ... Cleared tissue was washed in a PBS solution containing 0.1% SR2200 and then put into a PBS solution containing 0.1% SR2200 and a 1/1000 dilution of the nuclear stain TO-PRO-3 iodide (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Then cells were washed 3x for 5 min with PBS and incubated for 1 hour at RT with secondary antibodies (Supplementary Table 3) and Hoechst 33342 (Invitrogen, 62249). After 3 washes for 5 min with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR for the 5’ and 3’ fragments was carried out using 0.2 µl (1 U) Phusion polymerase according to the manufacturer’s instructions (ThermoFisher Scientific, Vilnius, Lithuania). PCR products were purified with E.Z.N.A ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...