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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Immunology 2024Quote: ... after which the cells were resuspended into 1-5 mL FBS and counted on a Countess (ThermoFisher Scientific) with trypan blue ...
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bioRxiv - Immunology 2024Quote: ... 5 x 106 to 1 x 107 cells per sample were lysed by RIPA buffer (Thermo Fisher, 89900) and shaken on a rocker for 1 h at 4°C ...
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bioRxiv - Genomics 2024Quote: ... which included 5 µL of 4x TaqPathTM 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, MD, USA), 1 µL of 10 µM forward primer ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a tri-gas incubator maintained at 1% O2 was employed (Thermo Scientific, Heracell 150i, 1.0% O2, 5% CO2).
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bioRxiv - Microbiology 2023Quote: ... 1×106 293T cells were seeded prior to transfecting 5 μg of plasmid DNA using Lipofectamine 2000 (Invitrogen), according to manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% v/v fetal bovine serum (Eurx, Gdansk, Poland) and 1% penicillin/streptomycin (Thermo Fisher Scientific) at 37°C in a humidified incubator under 5% CO2 and 95% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 5% CO2 using either DMEM (HeLa and PANC-1: high glucose, GlutaMAX™ Supplement, pyruvate; ThermoFisher Scientific 10569010) or RPMI 1640 (Jurkat and PBMC ...
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bioRxiv - Immunology 2023Quote: ... 5% glycerol and then mixed at 9 volumes to 1 volume of 200× concentrate SYPRO orange (Thermo Fisher) diluted in the same buffer ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) heat-inactivated fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- suspension cells (ATCC CRL-3022 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with the same medium with the addition of 5 μg mL−1 zeocin (ThermoFisher Scientific). For neuronal maturation ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES (pH 7.4)) with 5 µm CellROX Green (Thermo Fisher Scientific, C10444). Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... Both cell lines were cultured in MAT/P (US patent No. 4.816.401) supplemented with 5% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO) and incubated at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... with added 5 mL (1% v/v) P/S (Catalog: 15140-122, Thermo Fisher Scientific, Waltham, MA, USA), 60% Knock-out Serum (Catalog ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The sample (5 μl of 1 μg/μl) was analyzed in a QExactive-Orbitrap mass spectrometer (Thermo Scientific) using data-independent acquisition (DIA) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.3 [Sigma], 75 mM NaCl [Ambion], 1 mM GTP [Thermo Scientific], 3 mM MgCl2 [Ambion], 10 mM DTT [Thermo Scientific] ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3 times with PBS and incubated with appropriate fluorescent-labeled secondary antibodies (1:1000 dilution in 0.5% BSA, Life Technologies Inc) and the nuclear marker DAPI (Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: ... Detached cells were transferred into medium at 3:1 ratio of mTeSR1 to Neural Induction Medium (NIM) that consists of DMEM/F12 with 1x N2 supplement (ThermoFisher, 17502048), 1x non-essential amino acid (NEAA ...
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bioRxiv - Immunology 2021Quote: ... for 3 days and cultured in RPMI 1640 medium supplemented with 10% of FBS and 1% penicillin/streptomycin (all Gibco, Thermofisher). To obtain monocytes ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed daily and the cells passaged every 3–4 days at a ratio of 1:4 using the StemPro EZPassage tool (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... tissues were incubated at 37 oC with shaking in 0.14 Wunsch units/ml Liberase Blendzyme 3 (MilliporeSigma) and 1 x antibiotic/antimycotic (Thermo Fisher Scientific) in DMEM/F12 medium (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with 1x PBS (3 times; 5minutes each) and incubated with donkey anti-mouse Alexa Fluor Plus 488 IgG (1:500, Thermo Fisher) and donkey anti-rabbit Alexa Fluor Plus 647 IgG (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... a third dataset (Dataset 3 in Supplementary Table 1) was collected on a 300 kV Titan Krios microscope (Thermo Fisher Scientific) equipped with a K3 detector (Gatan ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in a ratio of 3:1 and total RNA was extracted with Ribopure Yeast Kit (Ambion, Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... Enzymatic treatments were done in staining buffer supplemented with 3 mM magnesium chloride with 1:200 dilutions of RNase T1 (EN0541, Thermo Fisher), RNase III (ShortCut RNase III ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Biochemistry 2019Quote: ... cells were cultured for 48h in the absence and for 3 weeks in the presence of 1 mg/mL G-418 selection antibiotic (Thermo Fisher). Stable cells expressing GFP-fusion proteins were trypsinized and single GFP+cells with scatter properties (FSC and SSC ...
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bioRxiv - Immunology 2019Quote: ... 15 μg of pMul1-FLAG and 15 μg of pAsc-Myc mixed with FuGENE HD at a ratio of 1:3 in 500 μl Opti-MEM (Thermo Fisher) were transfected ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed in 0.3% PBS-T 3 times for 20 minutes before being transferred to a solution containing Neurotrace 640/660 (Invitrogen, 1:800) prepared in 0.3% PBS-T for 2-3 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... or a mouse anti-HA (MBL, M180-3, 1: 200) antibody followed by incubation with fluorescently labeled secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were then washed 3× 10 min with PBS and incubated with the appropriate AlexaFluor-conjugated secondary antibody (1:200; Invitrogen/ThermoFisher Scientific) for 2h ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
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bioRxiv - Neuroscience 2020Quote: ... Dishes were washed 3 times with PBS-Triton X and incubated with Alexa-Fluor 488 conjugated secondary antibody 1:1000 (Molecular Probes-Invitrogen) in dilution buffer for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... slices were rinsed in 0.1% t-PBS for 3 washes and then incubated in AlexaFluor 488 goat anti-chicken (1:400, Life Technologies, A11039), AlexaFluor 555 goat anti-rabbit (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg/ml in sterile water Gibco 10977035, 1 hour at 37°C followed by 3 washes with PBS Gibco 10010015) / Laminin (Gibco 23017-015 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Genomics 2021Quote: ... This was measured by comparing cycle numbers against 1/3 of the maximum fluorescence intensity using real-time PCR graphs on ABI7900 (Applied Biosystems). After the additional PCR cycles ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...