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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
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bioRxiv - Biochemistry 2023Quote: ... followed by staining with secondary anti-Rabbit antibodies (1:500) conjugated to Alexa Fluor 555 (Goat anti-Rabbit IgG, Superclonal Recombinant Secondary Antibody, Alexa Fluor 555, Thermofisher, # A27039) for 30 minutes on a shaker at Room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (Thermo Fisher Scientific) at 1:500 for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse macrophage RAW 264.7 cells and mouse myoblast C2C12 cells were cultured in DMEM medium (Gibco); colorectal carcinoma COLO 741 cells and B-CLL MEC1 cells were cultured in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cytokines were measured using a mouse cytokine 10-Plex Panel kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Alexa A647 (1:1000; goat anti-mouse IgG Alexa Fluor 647; Invitrogen #A21236) for 2h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-CLIMP63 (followed by anti-Rabbit/Mouse/goat Alexa-dye coupled antibodies (1:400, Invitrogen). SiR-lysosome was used to visualize lysosomes in live microscopy (1:2000 ...
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse-(H&L)-Alexa488 and Goat anti-Mouse-(H&L)-Alexa647 secondary antibodies (Invitrogen), Alexa488-conjugated phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated in 1:1000 anti-mouse AlexaFluor488 or anti-mouse AlexaFluor594 (Thermo Fisher Scientific) and 4’,6-diamidino-2-phenylindole counterstain (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302, 1:250, donkey anti-mouse 488 Life Technologies, A21202, 1:1000), and CX3CR1 (PE-rat ...
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bioRxiv - Immunology 2022Quote: ... WT mouse CD8+ T cells were activated with anti-mouse CD3/CD28 dynabeads (11453D, Thermo Scientific) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently with fluorophore-labeled anti-mouse (goat anti-mouse Alexa Fluor 488; 1:2000; Invitrogen) and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse-IgG2 Alexa 568 and goat anti-mouse-IgG1 Alexa 488 (1:500, Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... Mouse monoclonal antibodies were detected with goat anti-mouse IgG HRP-linked antibody (61-6520; Invitrogen) diluted 1:5000 (v/v) ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were analyzed by SDS-PAGE followed by Coomassie-staining or western blot using an anti-His primary antibody (Thermo Fisher Scientific, Waltham, MA, USA), HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific, Massachusetts, USA), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was then incubated with purified 6x-His-TEV protease E106G (Cabrita et al. 2007) and 500 μM TCEP (Thermo Fisher Scientific, product number 77720) for 20-24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Biophysics 2020Quote: ... and anti-mouse Alexa 594 (Invitrogen). Images were captured at room temperature with a Zeiss confocal system (LSM510 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-CD3 (ThermoFisher; 1:200), Rabbit anti-IBA1 (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-CD68 (ThermoFisher; 1:100), Rabbit anti-VIM (RayBiotech ...
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bioRxiv - Cell Biology 2020Quote: ... donkey anti-mouse Alexa647 (Invitrogen; A31571), donkey anti-goat Alexa488 (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2020Quote: ... α-Mouse IgG2b Alexa647 (ThermoFisher, 1:400), α-Mouse Alexa488 (Thermofischer ...
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bioRxiv - Cell Biology 2020Quote: ... α-Mouse IgG1 Alexa488 (ThermoFisher, 1:500), α-Mouse IgG2b Alexa647 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse-HRP (Invitrogen, 31430) (1:10,000) ...
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bioRxiv - Microbiology 2021Quote: ... magnetic Dynabeads Pan Mouse IgG (Invitrogen) (1×108 beads / 0.25 ml ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-GAPDH (1:1000, Invitrogen), and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... goat-anti-mouse IgG HRP (ThermoFisher), and rabbit-anti-rat IgG HRP (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... mouse IgG1 (Life Technologies, 1:20) and for HVEM ...
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bioRxiv - Microbiology 2019Quote: ... or mouse anti-SAG1 (Thermo Fisher) at a dilution of 1:5000 for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-Pgk1 (22C5D8) (Life Technologies), mouse anti-MBP monoclonal (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... donkey anti-mouse AF488 (Molecular Probes), AF568 conjugated donkey anti-mouse ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-V5 (1:200, Invitrogen), rabbit anti-Cnn (1:500 (Heuer et al. ...
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bioRxiv - Neuroscience 2020Quote: ... GeneChip Mouse Gene 2.0ST Arrays (Affymetrix) were used ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse 488 (#A32723; Invitrogen), goat anti-mouse 555 (#A32727 ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse 647 (#A32728; Invitrogen), goat anti-rabbit 647 (#A32733 ...