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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2022Quote: ... Cardiac muscle was detected with a rabbit recombinant polyclonal primary antibody to cardiac troponin I (cTnI, Invitrogen, 1HCLC, 1:600) and a donkey anti-rabbit antibody conjugated to the fluorophore Alexa 647 nm (Invitrogen A31573 ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Neuroscience 2024Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 488/555/647 Conjugate (CTB-488/555/647, 0.1% wt/vol, 80 nL, ThermoFisher C34775/C34776/C34778 ...
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bioRxiv - Molecular Biology 2023Quote: Introduction of recombinant genes into the parental HEKR4 cell line was performed according to the manufacturer’s guidelines (Thermo Fisher Scientific). In brief ...
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bioRxiv - Systems Biology 2023Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cell Biology 2023Quote: Pooled HUVEC sample sets were grown to confluence before treatment with or without recombinant tumour necrosis factor alpha (TNF; 10 ng/mL) (ThermoFisher) in cell culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant bacmid DNA that carried the mini-HA domain was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant baculovirus generation and large-scale expression in Sf9 cells were carried out using the Bac-to-Bac™ (Invitrogen) expression system.
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic fragments were further shuffled into the pLenti-Gateway vector via Gateway LR recombinant reaction using Gateway LR Clonase II Enzyme Mix (Invitrogen). In the case of KCNA2 and OVOL1 ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant PrP substrates containing the D178N or E200K mutations were created by site-directed mutagenesis with the GeneTailor system (Invitrogen) using the bank vole (BV ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
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bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed with mouse anti-HA (12CA5) and mouse anti-Pgk1 (22C5D8, Molecular Probes), followed by probing with mouse anti-Pgk1 (22C5D8 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse primary antibody diluted 1:10,000 and anti-mouse-HRP secondary antibody (Thermo Fisher Scientific) diluted 1:50,000.
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 555 Mouse and Alexa Fluor 488 Mouse secondary antibodies were obtained from Invitrogen. The polyclonal antiserum to the recombinant human megalin cytoplasmic domain (anti-megT ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The probes for mouse TNF-α (Mm00443260_g1) and mouse β-actin (Mm00607939_s1) (Thermo Fisher Scientific) were used ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse IgG/IgM AlexaFluor 488 and anti-mouse IgG/IgG2α AlexaFluor 546 (both Invitrogen) were used ...
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bioRxiv - Immunology 2022Quote: ... and anti-pp65 antibody (mouse, Argene) combined with goat anti-mouse antibody (AF488, Thermo Fisher), respectively.
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bioRxiv - Genetics 2020Quote: ... cells were incubated in secondary antibodies Mouse Texas Red X (ThermoFisher, anti-mouse, 1:500) and Cy5 (anti-rabbit,1:500) ...
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bioRxiv - Immunology 2020Quote: Mouse serum or plasma was assessed via the IgG (Total) Mouse ELISA Kit (Thermo Fisher), according to the manufacturer’s directions ...
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bioRxiv - Immunology 2023Quote: ... in PBS1X containing MOM reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution, Invitrogen, R37621) at RT ...