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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: eGFP/Luc human iPSC-NSCs were seeded at a density of 1 x 104 cells per well in a Geltrex (Life Technologies) coated 96-well plate (flat bottom ...
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bioRxiv - Cell Biology 2024Quote: Cytokine secretion of PMA treated THP-1 cells was analyzed using Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) and measured with xMAP LuminexTM system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-YB-1 and EGFP-YB-1 (S1 File) in human cells were synthesized and subcloned in the vector pcDNA3.1(+) at GeneArt (Thermo Fisher Scientific). FLAG-M2-2 gene was inserted between the NcoI and AflII restriction sites ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... in imaging medium (custom; DMEM High-glucose [Gibco] without Riboflavin and Phenolred containing 10% [w/w] FCS [Gibco] ...
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FCS (Gibco™ Newborn Calf Serum [heat inactivated], of New Zealand origin; Thermo Fisher Scientific), 5% AA (Gibco® MEM) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: The purified Fc fusion baits were captured on 96-well protein-G coated plates (Thermo Fisher Scientific 15131). The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500) ...
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bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
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bioRxiv - Microbiology 2021Quote: ... ATCC (Cat# CRL-3216) were maintained with growth media (DMEM supplemented with 10% fetal calf serum (FCS, Invitrogen) and 100 U/ml penicillin and 0.1 mg/ml streptomycin (P/S ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... the copy number was quantified independently by performing FCS using Alexa Fluor 568 NHS ester (Thermo Fisher Scientific) to measure the confocal volume and using the cells that transiently-express mCherry alone to convert fluorescence intensity to the concentration.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
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bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
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bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco] ...
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bioRxiv - Immunology 2019Quote: RMA and RMA/s thymoma cells (cultured in Iscove’s modified Dulbecco’s medium supplemented with 10% FCS; Life Technologies) were labeled with Cell Trace Blue or Cell Trace Violet ...
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bioRxiv - Cell Biology 2020Quote: ... and the interface containing stromal cells was washed in DMEM/F12/ 10% fetal calf serum (FCS, Invitrogen, USA) and 1% primocin (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Zoology 2021Quote: ... Cells were then resuspended in L-15 (Leibovitz) medium containing 10% heat-inactivated fetal calf serum (FCS, Gibco) and antibiotics (penicillin–streptomycin ...
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bioRxiv - Cell Biology 2020Quote: MDA-MB231 and HeLa cells were cultured in 4.5 g/L glucose DMEM supplemented with 10% FCS (Gibco), 10 U/mL penicillin (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated hACE2-Fc was prepared using EZ-Link™ Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Caco-2WT and Caco-2ΔHk2 cells were cultured in MEM with 20% (v/v) FCS purchased from Gibco/Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2.
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450nm (OD450) using an accuSkan FC microwell plate reader (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2022Quote: ... was used to measure the m6A level in total RNA by MultiSkan FC microplate reader (Thermo Fisher, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
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bioRxiv - Physiology 2024Quote: ... The isolated cells were plated (0.1 % gelatine-coated T75 flasks, approx. 1.0×107 cells) and cultured in 10 % FCS-containing DMEM (Gibco). Medium was changed every 3 days ...
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bioRxiv - Microbiology 2023Quote: ... Polyclonal goat anti-pig immunoglobulin G (IgG) Fc Secondary Antibody HRP (Invitrogen by Thermo Fisher Scientific, Waltham, USA) was diluted 1:15.000 in blocking buffer and strips were incubated for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Both iPSCs classes were cultured on 0.1% gelatin-coated dishes in FCS/LIF medium (DMEM+Glutamax (61965059, Gibco), 15% fetal calf serum (Pansera ES ...
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bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
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bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
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bioRxiv - Biophysics 2023Quote: ... supplemented in media (DMEM with 10% FCS and penicillin, Life Technologies, final conc. 50 U/mL of penicillin) for 2 hours at 37° C ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with FC block for 30 minutes and stained with anti-MECA79 (53-6036-82, Invitrogen), anti-GP38 (127423 ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were incubated at 30 °C and OD600 was measured using a Multiskan FC Microplate Photometer (Thermo Fisher). For the evaluation of chemical compounds ...
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bioRxiv - Immunology 2023Quote: ... The idiotype antibodies and Fc- fusion protein were conjugated in house with Dylight650 antibody labelling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Immunology 2022Quote: ... S.Tm were washed with PBS and reconstituted in DMEM/F-12 (STEMCELL) supplemented with 3% FCS (Thermo Fisher). For 2D enteroid-derived monolayer infections ...