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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/mL Human Recombinant EGF (Gibco) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and OxPhos Human WB antibody cocktail (Thermofisher 458199). For visualization ...
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bioRxiv - Cancer Biology 2024Quote: ... human bFGF (Thermo Fisher Scientific, Cat. No. PHG0261), human EGF (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human FGF-basic (Fisher Scientific)] ...
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bioRxiv - Immunology 2024Quote: ... followed by globin reduction using GlobinClear Human (ThermoFisher). To generate sequencing libraries ...
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bioRxiv - Neuroscience 2024Quote: ... and a panel of ProcartaPlex Human Simplex (Invitrogen) beads ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL human basic FGF (Gibco, PMG0035) and 25 ng/mL FGF10 (R&D ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Bioengineering 2022Quote: The human monocyte cell line THP-1 was purchased from ATCC (TIB-202) and routinely cultured in RPMI 1640 medium (Life Technologies) containing 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... Cells were re-suspended at 100µL/well with 1:1000 diluent with anti-human IgG-Alexa 488 (#A28175, ThermoFisher, Waltham, MA). Plates were incubated for 1 hour at 4°C ...
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bioRxiv - Genomics 2019Quote: ... The digested tissue was mixed with 40 ml of CRB (1% human albumin (CSL Behring) in 40 ml of DPBS -/- (Gibco, #14190094)) and shaken vigorously to liberate the stromal cells ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Biochemistry 2019Quote: ... DSF was performed on human BACE1 (1 μg) using 96-well qPCR plates (AB Biosystems, UK) with 20x Sypro Orange (Invitrogen, UK) in 50 mM sodium acetate ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: The region from −2662 to −1 bp (relative to translation start site) of the human TMEM135 promoter was synthesized by Life Technologies. Additionally ...
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bioRxiv - Immunology 2021Quote: ... at a concentration of 1×105 cells/100μl of complete medium with or without Human T-Activator CD3/CD28 Dynabeads® (ThermoFisher Scientific). NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes ...
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bioRxiv - Neuroscience 2021Quote: The medial and baso-lateral amygdaloid nuclei were dissected from the mesial superior temporal lobe for 0.75-1 cm thick coronal slabs of frozen human brains on dry ice using a stainless steel punch (8mm diameter Thermo Fisher Scientific Integra Miltex standard biopsy punch) ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Bioengineering 2022Quote: ... a human keratinocyte cell line featuring constitutive expression of human telomerase reverse transcriptase (hTERT) were cultured in FAD medium (3:1 DMEM : Ham’s F12 Medium (ThermoFisher®, 11320074), supplemented with 1% PS ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/4500) (all from Jackson Immunosearch Laboratories, Inc.) or goat anti-human IgG (γ-chain specific) HRP conjugate (1/50 000) (Invitrogen) were added and samples were incubated at 20-24°C for 60 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 TM were resuspended in 1 mL RPMI media and either left unstimulated or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Immunology 2019Quote: ... or 1×105 (96-well plate) naïve CD8+ T-cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 beat to T-cell ratio and cultured in 1 ml (48WP ...
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bioRxiv - Bioengineering 2021Quote: DiPS 1016 SevA hiPSC line derived from human skin fibroblasts were seeded and kept in culture on Geltrex (1% Invitrogen, USA)-coated culture flasks using mTeSR (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Physiology 2019Quote: ... Approximately 20-40 mg of human ventricular myocardium was homogenized in 1 mL of Trizol (Life Technologies #15596-026, Carlsbad, CA) with a TissueLyser LT (Qiagen N.V ...
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bioRxiv - Genetics 2020Quote: ... hybridization mixtures were created from 5ul of the nick translated fluorescent RNA probe mixed with 10ul of human Cot-1 (ThermoFisher #15279011) and 2ul of Salmon Sperm DNA (ThermoFisher #15632011) ...
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bioRxiv - Immunology 2021Quote: ... or 1×105 (96-well plate) naïve CD8+ T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 bead to T cell ratio and cultured in 1 ml (48-well plate ...
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bioRxiv - Microbiology 2020Quote: ... murine alveolar macrophages MH-S (ATCC CRL-2019) and human monocytes THP-1 (ATCC TIB-202) were grown in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco 21875) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... The human leukemia monocytic cell line THP-1 16 was cultured in Milieu Roswell Park Memorial Institute (RPMI) medium (ThermoFisher Scientific) enriched with 10% fetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... From live single cells with top 30% protein expression (evaluated by EGFP signal) the MFI of the Alexa Fluor 647-conjugated goat anti-human IgG (1:400, Life Technologies) was calculated.
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... samples were washed in PBS and a mixture of goat anti-Human Alexa488 conjugated secondary antibody (Thermo Fisher, diluted 1:2000) and DAPI (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... T cells (1 million cells/ml) were activated with Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) in culture medium ...