Labshake search
Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... using the PureLink HiPure Plasmid MidiPrep kit (Invitrogen, Thermo Fisher Scientific). Sequences near the AgeI and KasI cloning sites were confirmed by Sanger sequencing in both directions as described above ...
-
bioRxiv - Microbiology 2021Quote: ... using the PureLink HiPure Plasmid MidiPrep kit (Invitrogen, Thermo Fisher Scientific). Sequences near the AgeI and KasI cloning sites were confirmed by Sanger sequencing in both directions as described above ...
-
bioRxiv - Microbiology 2020Quote: ... 4 µg plasmid and 6 µl Turbofect (Thermo Fisher Scientific, USA) were combined ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmid s were purified using the GeneJET Miniprep kit (Thermo Scientific). DNA fragments were purified using the Purelink PCR purification kit (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... plasmid DNA and Expifectamine were mixed in Opti-PRO SFM (Gibco) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The plasmid vectors (0.265 pmol) were transfected using Lipofectamine 3000 (Invitrogen) into HEK293T cells cultured in Dulbecco’s Modified Eagle’s Medium (Gibco ...
-
bioRxiv - Molecular Biology 2020Quote: ... plasmids were extracted using the miniprep kit (Fisher Scientific, cat # FERK0503) and sent to DNA sequencing to verify the plasmid sequence.
-
bioRxiv - Immunology 2021Quote: ... coli and purified using a GeneJET plasmid miniprep kit (Thermo Fisher). To express anti-DEC205-OVA ...
-
bioRxiv - Genomics 2021Quote: ... Transfect the constructed plasmid into the cells(Invitrogen, Cat. No. L3000075), and observe the expression under a fluorescent microscope 24 hours later.
-
bioRxiv - Genomics 2021Quote: ... cells were transfected with individual reporter plasmid using Lipofectamin 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The pDonor221 and pLenti6/V5- DEST plasmids were purchased from Invitrogen. The pENTR1A-GFP-N2 (#19364 ...
-
bioRxiv - Neuroscience 2020Quote: ... pRSVr and pMDLg-pRRE helper plasmids using Lipofectamine2000 (Thermo Fisher Scientific) into HEK293T cells ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmid transfections into 293FT cells were performed with LipofectaminTM 2000 (Invitrogen). One day later ...
-
bioRxiv - Biophysics 2022Quote: ... 200 ng of plasmid DNA and 0.4 μL P3000 reagent (Invitrogen) were diluted in 15 μL pre warmed opti-MEM and vortexed briefly ...
-
bioRxiv - Developmental Biology 2022Quote: ... plasmids were introduced into ElectroMAX Stbl4 Competent Cells (Life technologies #11635018) using MicroPulser Electroporator (Bio-Rad #1652100 ...
-
bioRxiv - Cell Biology 2022Quote: ... SUN2 cDNAs were cloned into the pcDNA5-FRT-TO plasmid (Invitrogen). For CTDNEP1-rescue experiments a lentiviral vector was used with the expression of CTDNEP1 being driven by the EF-1α promoter ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Plasmid extraction and DNA gel purification kits were purchased from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... The fragment was cloned into the pUC19 plasmid (Thermo Fisher Scientific) using the Hindlll/BamHI restriction sites ...
-
bioRxiv - Molecular Biology 2022Quote: ... purified and cloned in the plasmids pCR8/GW/TOPO® (Invitrogen) using the primers listed in Supplementary Table S2 ...
-
bioRxiv - Biochemistry 2022Quote: ... U2OS cells were transfected with plasmid DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and analysed 24 hours after transfection ...
-
bioRxiv - Microbiology 2022Quote: ... PCR and cloned into the TOPO-TA plasmid (Thermo Fisher Scientific). The human wild-type GBA1-containing TOPO-TA plasmid was then used as a template to generate the N370S and L444P GBA1 variants ...
-
bioRxiv - Neuroscience 2022Quote: ... or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were fixed using 4% paraformaldehyde (PFA ...
-
bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmids were cut using appropriate restriction enzymes (Fast Digest-Thermo Scientific), dephosphorylated with FastAP and purified from an agarose gel with a Zymoclean Gel DNA Recovery kit (Zymo Research) ...
-
bioRxiv - Microbiology 2023Quote: ... pyciF was purified (GeneJET plasmid miniprep kit, Thermo Fisher Scientific, Lithuania) and confirmed by restriction digestion to visualize the insert release and Sanger sequencing (Aggrigenome ...
-
bioRxiv - Molecular Biology 2022Quote: ... The expression kit containing pPICZαB plasmid was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with pCBA-SceI plasmid using Lipofectamine 2000 (Invitrogen). Cells were harvested 48 hrs post-transfection ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 500 ng TORNADO Broccoli expression plasmid using Lipofectamine 3000 (Invitrogen). After 48 and 72 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid transfection was performed using Lipofectamine 3000 Reagent (Thermo Fisher Scientific), in accordance with the manufacturer’s instructions.
-
bioRxiv - Biophysics 2022Quote: ... the vector backbone was amplified from the pcDNA5-FRT plasmid (Invitrogen), the stdMCP-stdGFP fragment was amplified from the pUbC-NLS-HA-stdMCP-stdGFP plasmid (Addgene ...
-
bioRxiv - Cell Biology 2022Quote: Plasmid DNA was electroporated into iPSDMs using the Neon system (Invitrogen). iPSDM were resuspended at 1.5×106 cells in 100 µl buffer R ...
-
bioRxiv - Microbiology 2024Quote: ... and ligated into pBAD/Thio-TOPO plasmid vector (Thermo Fisher Scientific). The plasmids were transformed into TOP10 competent cells (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... 250-500 ng plasmid and 1 μL Lipofectamine 3000 reagent (Invitrogen) were mixed in Neurobasal medium for a well and incubated for 15 min at room temperature ...
-
bioRxiv - Synthetic Biology 2024Quote: ... pAAV cTnT HyPerDAAO plasmid was cleaved with SacI (Thermo Scientific, FD1133) and BglII restriction enzymes (Thermo Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... Plasmid isolation was done employing the GeneJetTM Miniprep kit (ThermoFisher Scientific) and sequencing was performed by the DNA sequencing service at the chair for biochemistry ...
-
bioRxiv - Microbiology 2024Quote: ... The plasmids were transformed into TOP10 competent cells (Thermo Fisher Scientific). Transformants were screened by colony PCR using Bd37 internal primer Bd37-SQF1 (supplement table1 ...
-
bioRxiv - Immunology 2024Quote: ... The two plasmids were transiently transfected into FreeStyle 293 cells (Invitrogen) using polyethylenimine followed by treatment with 5 μM kifunensine to ensure uniform high-mannose glycosylation ...
-
bioRxiv - Plant Biology 2024Quote: ... and transferred into Ti plasmids by multisite Gateway LR reaction (Thermofisher) for simultaneous expression of right and left platinum TALENs fused to the Arabidopsis delta prime subunit mitochondrial presequence ...
-
bioRxiv - Microbiology 2024Quote: ... Individual reporter plasmids were amplified by EquiPhi29 polymerase (Thermo Fisher Scientific) and transformed into recipient B ...
-
bioRxiv - Cell Biology 2024Quote: ... and the aforementioned plasmid in Opti-MEM medium (Thermo Fisher Scientific). This mixture was allowed to interact at RT for 5 minutes prior to application ...
-
bioRxiv - Microbiology 2023Quote: ... The FLAG-pTBK1-HA plasmid was generated by Gateway cloning (Invitrogen), using the FLAG-pTBK1 as template for generation of the entry clone (forward primer ...
-
bioRxiv - Neuroscience 2024Quote: ... Plasmid DNA was linearized using the Mlu1 restriction enzyme (Thermo Fisher) and then in vitro transcribed using the Sp6 RNA polymerase (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Amplified DNA fragments were cloned into pENTR/D-TOPO plasmids (Invitrogen) as recommended by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... All vectors were purified using the GeneJet Plasmid Kit (Thermo Scientific) and sequence verified by Sanger sequencing (PSOMAGEN) ...
-
bioRxiv - Molecular Biology 2024Quote: ... were transfected with 2.5 µg pLightswitch reporter plasmid and pMIR (Ambion). 24 hours post-transfection ...
-
bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was isolated using a midi-prep kit (Invitrogen; K210005). The integrity of the CRISPRi library was validated by Illumina Miseq through Cornell Genomics Facility (detailed library preparation steps are presented below in “Library validation and CRISPRi dropout screening”).
-
bioRxiv - Immunology 2023Quote: HeLa cells were transfected with receptor plasmids using Lipofectamine 3000 (Invitrogen) for 24 hrs ...
-
bioRxiv - Cell Biology 2024Quote: ... Plasmid transfection was performed using Lipofectamine 2000 or Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: Plasmids were constructed using Gateway assembly (Thermo Fisher Scientific, Waltham, MA). The pME’ clrn1 entry clone was generated by amplifying the full-length cDNA of clrn1 (ENSDART00000171202.2 ...
-
bioRxiv - Cell Biology 2024Quote: ... about 250-300 ng of plasmids were transfected using Fugene (Invitrogen). p53-TA-Luc (Clontech/Takara ...