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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Plasmids were constructed using the two-slot Gateway Cloning system (Invitrogen) and confirmed by restriction digest and sequencing.
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bioRxiv - Microbiology 2020Quote: Plasmids were cloned using Escherichia coli DH5α cells (Invitrogen, Karlsruhe, Germany). Construction of deletion mutants was performed by homologous recombination ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were linearized with the SacI restriction endonuclease (ThermoFisher Scientific, Canada) for 15 min at 37°C and used as templates for in vitro transcription ...
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bioRxiv - Immunology 2020Quote: ... Plasmids or siRNA were transfected into cells with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Varying amounts of plasmid and lipofectamine transfection reagent (Thermo Fisher Scientific) were combined separately in 125 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Transfections of plasmids were performed in triplicate with Lipofectamine 2000 (Invitrogen) as directed by the manufacturer with 10 pmol of siRNA (Control or On Target plus Smart Pool mouse FMR1 ...
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bioRxiv - Molecular Biology 2022Quote: ... or pcDNA5/FRT/TO-3XFL-ZFR and pOG44 plasmids (ThermoFisher Scientific) with TurboFect transfection reagent according to the manufacturer’s protocol (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5 μg of plasmid DNA and 5 μl P3000 reagent (Invitrogen) diluted in OPTI-MEM medium (Gibco ...
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bioRxiv - Systems Biology 2022Quote: Extracted plasmids were amplified using random hexamer primers (Thermo Scientific SO181) and φ29 DNA polymerase (NEB M0269L) ...
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bioRxiv - Cell Biology 2022Quote: ... or HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific, Waltham, MA) and sequenced to ensure that the correct modifications were in place.
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bioRxiv - Cell Biology 2022Quote: ... or a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). Primers were obtained from IDT (Integrated DNA Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with biosensor encoding plasmids using lipofectamine 3000 (ThermoFisher). Live-cell imaging was performed 48 hours post-transfection with a laser-scanning confocal microscope (FV1000 ...
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bioRxiv - Cell Biology 2022Quote: Transient transfection of plasmids was performed using Lipofectamine 2000 (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were amplified using Subcloning Efficiency DH5a competent cells (Life Technologies). Plasmid DNA was isolated using the HiPure Plasmid Maxiprep kit (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Cell transfection with plasmid DNA was performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 6 µg plasmid DNA in Optimem® transfection medium (# 31985062, Gibco) was used for each transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids and PCR products were purified using the GeneJET (Thermo Scientific) plasmid miniprep kit and gel extraction kit ...
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bioRxiv - Microbiology 2019Quote: ... Amplified DNA fragments were then ligated to pJET2.1 plasmid (Thermo Scientific), and the products transformed into E.coli DH5α ...
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bioRxiv - Biochemistry 2020Quote: ... All constructs were cloned into the pcDNA3.1(+)/hygro expression plasmid (Invitrogen). The Strep-FLAG pcDNA3.1(+)/G418 NEGR-1 and the anti-NEGR-1 primary antibody were kindly provided by Giovanni Piccoli (University of Trento ...
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bioRxiv - Cell Biology 2020Quote: ... EEA1 plasmids were generated using Gateway cloning system (Thermo Fisher Scientific), and EGFP-EEA1 plasmid as a template for cloning (Addgene ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR product was cloned into a pCR2.1-TOPO plasmid (Invitrogen) containing M13 primers and a T7 RNA polymerase transcription initiation site ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with the corresponding plasmid using Lipofectamine2000 (Life Technologies). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA plasmids were mixed with Lipofectamine LTX (15338100, Thermo Fisher Scientific) or Fugene HD (E2311 ...
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bioRxiv - Biophysics 2019Quote: ... 1-5 µL (10pg-100ng) pRSET-EmGFP plasmid (Invitrogen, CA, USA) was mixed with 25 µL thawed bacterial solution and incubated for 5-10 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... inhibitor and the plasmids were transfected with lipofectamine 3000 (Invitrogen, USA). Briefly ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... and prepped using a HiPure plasmid Midi or Maxi kit (Invitrogen).
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bioRxiv - Synthetic Biology 2021Quote: All part plasmids were either directly synthesised (GeneArt, Thermo Fisher Scientific) or assembled as complementary single-stranded DNA oligos annealed together ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were transfected cells using Lipofectamine 2000 Reagent (11668-019, Invitrogen), FuGENE HD Transfection Reagent (E2311 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting sgRNA plasmid was linearized by FastDigest DraI (Thermo Scientific) and served as template for sgRNA in vitro transcription using the T7 MEGAshortscript Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid DNA and siRNA oligonucleotides were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... Plasmid constructs were introduced via Neon electroporation (Invitrogen, Carlsbad, CA, USA) as previously described (16) ...
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bioRxiv - Molecular Biology 2019Quote: ... the respective plasmids were digested with FastDigest PvuI (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2021Quote: ... with vacuum manifold and GeneJET Plasmid Midiprep Kit (Thermo Fisher Scientific) were used for higher yields ...
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bioRxiv - Biochemistry 2019Quote: ... 0.5 μg plasmid DNA in 200 μl Opti-MEM (Life Technologies). After incubation for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... which were then purified using the Purelink Plasmid Midiprep Kit (Invitrogen) and introduced into rice protoplasts as described previously (Kawano et al ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections with plasmid DNA were performed using Lipofectamine2000 reagent (Thermo Fisher) according to manufacturer’s recommendation ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: ... p3000 and the plasmids were previously diluted in OptiMEM 1X (Gibco) (a Reduced-Serum Medium) ...
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bioRxiv - Genetics 2021Quote: ... or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA) according to the provided protocols ...
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bioRxiv - Genomics 2021Quote: ... were cloned and purified with HiPure Plasmid Midiprep Kit (Invitrogen; K210005).
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were isolated using Gene-JET Miniprep Kit (Thermo Scientific, K0503). Isolated plasmids were verified with Sanger sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were carried out using Lipofectamine 2000 Transfection Reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cloned into Zero Blunt® TOPO® plasmid (Thermo Fisher Scientific), and sequenced.
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bioRxiv - Plant Biology 2021Quote: ... cloned into Zero Blunt® TOPO® plasmid (Thermo Fisher Scientific), and sequenced ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids of interest were transfected using Lipofectamine 3000 (Thermo Fisher Scientific) or jetPRIME® (Polyplus transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 3000 (Life Technologies) according to the manufacturer instructions.
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bioRxiv - Biochemistry 2021Quote: ... DNA was purified using the PureLink plasmid midiprep kit (Invitrogen, CA), filtered through a 0.22 μm filter ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using a GeneJET miniprep kit (ThermoFisher, K0503) and sequence-confirmed with Sanger sequencing on an Applied Biosystems 3730 Genetic Analyzer.
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid was transfected into cells using the Lipofactamine 3000 reagent (ThermoFisher) following the manufacture’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 5ug of plasmid library and 175μl of ssDNA (UltraPure, Thermo Scientific) were added to the cells ...