Labshake search
Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... manually minced and enzymatically digested in an orbital shaker for 2 hours at 37°C in digestion mix (9.2 ml DMEM/F12, 5% FCS, 1% Penicillin/Streptomycin, 25 mM HEPES (Gibco, cat. #15630056) and 300 U/ml Collagenase IV (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... major Friedlin (MHOM/SU/73/5-ASKH) strain (1×105 logarithmic promastigotes per mL) were cultured at 26 °C in M199 medium (Gibco, USA) (pH 7.4 ...
-
bioRxiv - Immunology 2023Quote: ... in PBS for 1 h at 25°C followed by permeabilization in 5% BSA in the presence of 0.05% Tween-20 (Thermo Fisher Scientific) for 15 minutes at 25°C ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1% Penicillin Streptomycin at 37 °C and 5% CO2 and split when confluent using Trypsin-EDTA (0.05%) (Life Technologies, 25300054). When indicated ...
-
bioRxiv - Microbiology 2024Quote: ... THP-1 monocytes were cultured at 37°C with 5% CO2 to confluence in RPMI media (MEDIUM 1640 CE, Gibco, 21875034) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific, Inc., Waltham, MA, USA) on a rotator for subsequent affinity chromatography via the C-terminal 6xHis-tag ...
-
bioRxiv - Microbiology 2022Quote: ... at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (Gibco), 5% Cosmic calf serum (Hyclone) ...
-
bioRxiv - Cell Biology 2022Quote: ... at 37°C for 10 minutes and blocked with DPBS containing 5% BSA and 5% normal goat serum (Gibco), for 30 mins at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... 5 μg total RNA were denatured for 5 mins at 95°C in RNA Gel loading dye (Thermo Scientific) before being separated on 1% agarose gels in 1X TBE (native ...
-
bioRxiv - Microbiology 2024Quote: ... at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (Gibco), 5% Cosmic calf serum (Hyclone) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and LCPNS-SIIN-Cxcl1KO cells were cultured at 37°C under 5% CO2 / 5% O2 in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 medium, Gibco) supplemented with 1% N2 (Gibco) ...
-
bioRxiv - Microbiology 2023Quote: ... at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (Gibco), 5% Cosmic calf serum (Hyclone) ...
-
bioRxiv - Microbiology 2023Quote: ... host cells at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% fetal bovine serum (Gibco), 5% Cosmic Calf serum (HyClone) ...
-
bioRxiv - Immunology 2020Quote: ... mRNA concentrations were measured using Nanodrop One/One (Thermo Fisher), and 250 ng RNA of each sample was reverse transcribed to cDNA using the Primescript RT kit (Takara Bio ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with PBS and incubated for 3 min at 37°C with 1 mg/ml Alexa Fluor™ 647-Dextran (Invitrogen, REF D22914) without preincubation or acid stripping ...
-
bioRxiv - Cell Biology 2021Quote: ... were transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermo Fisher Scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
-
bioRxiv - Microbiology 2022Quote: ... 72°C for 20 seconds and final termination at 72°C for 5 min in a thermocycler (Applied Biosystems, USA). All PCR products were resolved and visualized by 1.5% to 2% w/v agarose gel electrophoresis ...
-
bioRxiv - Neuroscience 2024Quote: ... 60 minutes at 42°C followed by 5 minutes at 70°C or using the high-capacity cDNA reverse transcription kit (4368814, ThermoFisher) with the following programme ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4°C) and boiled at 95°C for 10 minutes before loading on to NuPAGE 4-12% Bis-Tris gels (Thermo Fisher).
-
bioRxiv - Biochemistry 2021Quote: ... denaturation at 95 °C for 10 min and gradually temperature decrease to 4 °C during 20 minutes in a thermal cycler (Applied Biosystems). Ribonucleoprotein complexes (RNP ...
-
bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...
-
bioRxiv - Cell Biology 2022Quote: ... 200µL 5-Ethynyl-2’-deoxyuridine (EdU, Molecular probes; 3g/L) was injected intraperitoneally in pregnant females 2 hours before embryo isolation.
-
bioRxiv - Immunology 2019Quote: ... 2 µL of the primer mix (5 mM dNTP [Invitrogen] ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 5.5 x 10-5 mol/L 2-mercaptoethanol (Gibco). For the endothelial potential assay ...
-
bioRxiv - Cell Biology 2020Quote: ... For 5-ethynyl-2’-deoxyuridine (EdU) incorporation assays (Life Technologies), 10μM EdU was included in culture for two hours ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) (Thermo Fisher Scientific, cat. #E10415) was diluted 2.5 mg/ mL in sterile PBS and injected intraperitoneally on days 3 and 4 post-injury at a dose of 10 μL/ g body weight ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific, Waltham, MA) was either injected i.p ...
-
bioRxiv - Cancer Biology 2022Quote: ... or 2 - 5 μg/ml Puromycin Dihydrochloride (Thermo Fisher, # A1113803) until all negative control cells were dead ...
-
bioRxiv - Microbiology 2022Quote: ... or 0.1 mM 5-ethynyl-2’-deoxyuridine (EdU; Life Technologies) was added for the time indicated in the text.
-
bioRxiv - Cancer Biology 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Barcelona, Spain) was added for 48h ...
-
bioRxiv - Zoology 2019Quote: ... containing 5 µM Fura-2 acetoxymethyl ester (Molecular Probes, Invitrogen) for 30 min at room temperature ...
-
bioRxiv - Zoology 2019Quote: ... containing 5 µM Fura-2 acetoxymethyl ester (Molecular Probes, Invitrogen) for 30 min at room temperature ...
-
bioRxiv - Biophysics 2020Quote: ... while GRN-5 was expressed in Origami 2 DE3 (Invitrogen) as fusion constructs with a thioredoxin-A and hexa-histidine tag (TrxA-Hisx6-GRN) ...
-
bioRxiv - Cell Biology 2021Quote: ... 2 ml of bovine collagen I (5 mg/ml) (Gibco), and 6.67 ml serum starvation medium were mixed on ice ...
-
bioRxiv - Cell Biology 2020Quote: ... For pulsed EdU (5-ethynyl-2’-desoxyuridine) (Thermo Fisher Scientific) incorporation ...
-
Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... and 2 μl of 5 × First strand synthesis buffer (Invitrogen) were added and incubated ...
-
bioRxiv - Cell Biology 2021Quote: ... 6 - diamidino-2-phenylindole (DAPI, 5 µg/ml, Life Technologies).
-
bioRxiv - Physiology 2022Quote: ... loading buffer containing 5% 2-mercaptoethanol (Fisher Scientific, O3446I-100). Samples were then subjected to SDS-PAGE on NuPAGE Novex 12% Bis-Tris gels (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 µL TaqMan Universal PCR Master Mix (2×) (Applied Biosystems), and 0.5 µL of each TaqMan Gene Expression Assay reagent ...
-
bioRxiv - Microbiology 2022Quote: ... Biotinylated RNA was bound 1:1 to Dynabeads My One Streptavidin T1 (Thermo Fisher Scientific) equilibrated in 10 mM Tris pH 7.5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
-
bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287, 4-nitroquinoline 1-oxide, Sigma N8141, hydroxyurea, Acros Organics 151680250 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Ago2 and IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A or G (ThermoFisher Scientific). After incubation ...
-
bioRxiv - Neuroscience 2021Quote: ... 1:40 and anti-GFP 1:400 Rockland 600-101- 215 were incubated 24h at 4° C followed by secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), Alexa 568 anti-Rabbit (Thermo Fisher A10042) ...
-
bioRxiv - Molecular Biology 2021Quote: ... was carried out overnight at 4° C followed by secondary antibodies (conjugated with Alexa Fluor 488 or Alexa Fluor 647 (1:250; Molecular Probes, Invitrogen) at RT for 4-5 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in secondary antibody diluted 1:500 in block (donkey anti-rabbit Alexa 568, Life Technologies A10042). Sections were again washed in TBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2008) at 4°C overnight and then incubated with Alexa Fluor 488-conjugated anti-rabbit antibody (1/800, Life Technologies, Oregon, USA). For in situ hybridization of the mouse Mesp1 and Mesp2 mRNA ...
-
bioRxiv - Genomics 2020Quote: ... at a dilution of 1:100 overnight at 4°C followed by donkey Alexa488-conjugated anti-rabbit secondary antibody (Thermo Fisher Scientific) at a dilution of 1:200 for 30min at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... 40 µm sections were collected using a cryostat and stained with primary antibody overnight at 4° C (1:1000 rabbit α-GFP, Life Technologies) and incubated with secondary antibodies (2h at room temperature ...