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Citations for Thermo Fisher :
3001 - 3050 of 7572 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: siRNA knockdown was performed as previously reported.20 9-12 week placental explants were placed into a 48-well plate with IMDM supplemented with 10% (v/v) FCS and gentamicin (50 μg/mL; Gibco). siRNA sequences targeting PRKCI (final concentration 0.2 μM ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 4mM EDTA and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), CD4-AF488 (clone GK1.5 ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... E14 mouse embryonic stem cells were cultured in plates coated with 1% gelatine in GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Dreireich, Germany) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen plugs were set at 37°C/5%CO2 for 10 minutes, and then fibroblast growth medium was added (DMEM medium supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Microbiology 2024Quote: ... and HKU1 NTD Fc + pA-LS at 50 μg/mL were incubated with StrepMAB-Classic (IBA) and goat αnti-mouse IgG HRP (Invitrogen) at a 4:2:1 molar ratio for 30 min on ice ...
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bioRxiv - Microbiology 2024Quote: The binding of KIRs-Fc fusion proteins to iRBCs was assessed by flow cytometric analysis with Attune NxT (ThermoFisher Scientific). Prior to the assay ...
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bioRxiv - Immunology 2024Quote: ... BHK21 cells were purchased from ECACC (85011433) and cultured in DMEM (supplemented with 10 % FCS, 10 mM HEPES (Gibco, 15630056), 1 mM Na-Pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Synthetic Biology 2021Quote: ... supplemented with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with cells ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific, MA, USA), and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific, MA, USA), BV421-conjugated mouse anti-human CD34 (Biolegend ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: Human PSCs resistant to neomycin were incubated with Accutase (Gibco) in 37°C for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Human staining panels included anti-CD3-Alexa 700 (Invitrogen, UCHT1), anti-CD19-APC (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... anti-human IgG secondary antibody labeled with Dylight 650 (Invitrogen) was applied at 1 to 1000 (∼0.5μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 20 ng/mL human heat stable sbFGF (Gibco, PHG0367) and split using accutase (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Genomics 2020Quote: FISH probes were made from human BACs (ordered from Invitrogen, RP11-479I13 for HSPA1A locus and RP11-173P16 for HSPH1 locus) ...
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bioRxiv - Immunology 2021Quote: ... goat anti-human Ig polyvalent antibody (10 μg/ml; Invitrogen), or purified GPIIbIIIa (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific), G-CSF ELISA kit (R&D Systems) ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: Human VIP2R (residues 24-438) was cloned into pFastBac (Invitrogen) with an N-terminal FLAG tag followed by a His8 tag ...
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bioRxiv - Immunology 2019Quote: ... labeling and hybridization to the Human GeneArray (Thermo Fisher Scientific) were performed according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Probes were mixed with Human Cot-1 DNA (Invitrogen, 15279011) and salmon sperm (Invitrogen ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The human neural stem cells (NSCs) were obtained from ThermoFisher/Invitrogen (N7800-100 ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... ELISA quantification of human IgG4 (#BMS2059, ThermoFisher Scientific, Waltham, MA) was carried out per manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... or other components of animal or human origin (ThermoFisher, USA). HEp-2 cells were initially seeded in growth medium (DMEM medium with 5% FBS (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... Human PDA cell lines were cultured in RPMI 1640 (Gibco) containing 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 µg of human Cot-1 DNA (ThermoFisher Scientific) and then denatured in deionized formamide (Merck ...
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bioRxiv - Physiology 2019Quote: ... and hybridization to Human GeneChip® HTA 2.0 Arrays (Affymetrix) were done as described previously (Mattiucci et al. ...