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Citations for Thermo Fisher :
2901 - 2950 of 7572 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Cancer Biology 2021Quote: The BCR-ABL-positive B-cell leukemia cell line BV173 (DSMZ, Braunschweig, Germany) was cultivated in RPMI1640 Supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco).
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bioRxiv - Cell Biology 2020Quote: ... the cell viability was determined using a micro plate reader (Thermo Scientific Multiskan FC, Thermo Fisher Scientific Oy, Ratstie, Finland) according to the manufacture’s protocol of CCK-8 ...
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bioRxiv - Cell Biology 2020Quote: HELA cells were plated in a 12-well plate in DMEM+ 10%FCS and transiently transfected using Lipofectamine3000 (Thermofisher, L3000001). Each transfection consisted of 250 ng expression plasmid (pcDNA3-control (Addgene ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
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bioRxiv - Microbiology 2022Quote: Unspecific Fc binding was blocked for 15 minutes on ice with unconjugated anti-CD16/CD32 (clone 93; Thermo-Fisher Scientific). Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Immunology 2019Quote: ... ATCC CRL-3216) cells were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated fetal calf serum (FCS, ThermoFisher Scientific) and Penicillin-Streptomycin (PS ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... The remaining tissue was let to sediment and the supernatant was collected in a tube containing the same volume of 10% FCS (Gibco)-HBSS+/+ and kept on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... grown in DMEM supplemented with 10% FCS and neurospheres (RNS, enriched in cancer stem cells) grown in DMEM/Ham’s F12 GlutaMAX (Life Technologies) supplemented with B27 and N2 additives (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... cell number enumerated with a hemocytometer and resuspended in 0.5 ml PBS supplemented with fetal calf serum (FCS, ThermoFisher Scientific) to a final concentration of 1 × 103 cells/ml in each well of a 24 well plate ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Biowhitaker) supplemented with 10% fetal calf serum (FCS) (v/v) and 1% penicillin/streptomycin (GIBCO). Inducible cell lines were selected on 5 μg/ml blasticidin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured and maintained at 37 °C in a humidified atmosphere of 5 % CO2 in air in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutMAXTM-1 containing 10 % Fetal calf serum (FCS) and 1% Penicillin-Streptomycin (10 000 U/ml) (Gibco Life Technologies ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Cancer Biology 2020Quote: ... BALB/c-derived A20 B lymphoma cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 supplemented with 10% FCS (GIBCO). For in vivo experiments A20 cell lines were injected i.v ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice with ice cold Cytoperm reagent and re-suspended in 1% fetal calf serum (FCS) in PBS (Invitrogen, 14190). Cells were subsequently analyzed on a CYAN flow cytometer (Beckmann Coulter).
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bioRxiv - Microbiology 2020Quote: ... Cells were maintained at 37°C in the presence of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and 1% penicillin–streptomycin (fresh medium) (all from Invitrogen). Cells were used between 10 and 35 passages ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Neuroscience 2020Quote: ... After washing chase medium (DMEM containing 10% FCS, Glutamax, P/S, 1 mg/mL methionine and 1 mg/mL cysteine (Gibco)) was added to cells and incubated for different time points ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... splenic NK cells were isolated and suspended in NK cell medium (phenol-red free RPMI 1640 containing 10% FCS, non-essential amino acids, L glutamine and sodium pyruvate, all from Gibco). The indicated target cells were labelled with 15μM Calcein-AM (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The overnight inoculum was diluted 1:33 in infection medium (DMEM + 10% FCS + HEPES (Life Technologies, cat. number: 15630-056)) to obtain OD600=1 after 3 h of incubation to give 1.5 × 109 bacteria/ml ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... University of Bristol) were grown in DMEM supplemented with 8% FCS and 8% (v/v) tryptose phosphate broth (Life technologies). Penicillin and streptomycin (90 IU/ml ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal calf serum (FCS) (Thermo Fisher Scientific, Hamburg, Germany), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Biopsies were washed in PBS and transferred into wells containing RPMI-1640 + 10% FCS + 20 μg/mL G418 (Thermo Fisher) + 20 U/mL Pen/Strep and cultured for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Blots were imaged using the LiCor Odyssey® Fc system after treatment with ECL Western Blotting-Substrat (Thermo Fisher, 32209).
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bioRxiv - Immunology 2022Quote: ... femurs and tibia of mice were flushed with complete medium (RPMI-1640 and 10% heat inactivated fetal calf serum (FCS) (Gibco). Single cell suspensions were incubated with 5 mL of red blood cell (RBC ...