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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Mouse OLFR139 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Germany) and activated with mouse anti-CD3 (5ug/ml) and mouse anti-CD28 (5ug/ml) (Gibco, Billings, MT, USA, 11132D) antibodies for 48-72 hours ...
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bioRxiv - Neuroscience 2023Quote: ... prior to incubation in secondary antibodies (goat-anti mouse IgG1 – Alexa 488 and goat-anti mouse IgG2b-Alexa 594, 1:1000 for both, Invitrogen). Endogenous tissue fluorescent was quenched via post-staining treatment with Sudan Black (0.01% ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... Cytokine levels of IL-17A and IFNγ were quantified from collected supernatant using Mouse IL-17A Uncoated ELISA and Mouse IFN gamma Uncoated ELISA (Invitrogen) Kits ...
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bioRxiv - Biophysics 2021Quote: ... Cultures were miniprepped using a GeneJET plasmid miniprep kit (Thermo Scientific, cat#K05053). Library completeness was verified by deep sequencing on a MiSeq (Illumina).
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bioRxiv - Biophysics 2021Quote: ... The generated plasmid was then mixed with CellfectinTM II reagent (Thermo Fisher Scientific) in a 1:1 molar ratio and transfected into adherent Sf9 insect cells at a density of 5 × 105 cells per millilitre ...
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bioRxiv - Biophysics 2021Quote: ... The indicated plasmid was transfected into U2OS cells by the Lipofectamine LTX (Invitrogen) according to the standard protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... into the pLX304 plasmid by the Gateway cloning system (Thermo Fisher Scientific, 11791020). For MSI1 overexpression ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient plasmid transfections were performed using Opti-MEM and Lipofectamine 2000 (Life Technologies), and cells were collected for experiments 24 h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... https://plasmid.med.harvard.edu/) The construct was shuttled into the pDEST15 gateway plasmid (Thermo Fisher) carrying an N-terminal GST tag for bacterial protein expression and purification.
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bioRxiv - Cell Biology 2020Quote: ... the p22 expressing plasmids were transfected using Lipofectamin Stem Transfection Reagent (Thermo Fisher). Halotag was labeled by JF635-HaloTag ligand (53) ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng of pFastbac plasmids were transformed into MultiBac DH10 cells (Invitrogen, 10361-012) and positive clones were selected on LB Ampicilin (100μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Expi293F cells were transfected with plasmid DNA using ExpiFectamine transfection reagent (ThermoFisher Scientific). At 72 h post-transfection ...
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bioRxiv - Biophysics 2020Quote: Cells were transiently transfected with plasmid DNA using Lipofectamine 2000 (PN: 11668030, Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were constructed using the GeneArt Seamless Cloning and Assembly Kit (Life Technologies) and propagated in E ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with ASOs and plasmids using Lipofectamine 3000 (Life Technologies, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 microgram of This plasmid was transfected into HeLa cells using cellfectin (Invitrogen) reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... which was cloned into the pCDNA5 FRT/TO plasmid (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with ASOs and plasmids using Lipofectamine 3000 (Life Technologies, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... The plasmid concentration was quantified using a Nanodrop 2000 (Thermo Scientific, Waltham, MA). One microliter of each dilution was used as a template for LAMP.
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bioRxiv - Molecular Biology 2020Quote: ... 1ug digested plasmid was added to mMessage mMachine SP6 reaction (Ambion, Austin, TX) followed by DNA digestion and RNA clean-up using RNA Clean and Concentrator kit (Zymo ...
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bioRxiv - Molecular Biology 2020Quote: Cells were transfected with plasmid DNA (Supplementary Table 3) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 µg of 53BP1-expression plasmids were transfected using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer guidelines ...
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bioRxiv - Genomics 2020Quote: ... The resulting plasmids were transfected into HeLa cells using Lipofectamine 2000 (Life Technologies). These cells were engineered to contain a single loxP-flanked cassette within their genome (Khandelia et al. ...
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bioRxiv - Biochemistry 2019Quote: pET151/D-TOPO plasmids containing the sequences of interest were acquired from Invitrogen; fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene ...
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bioRxiv - Genetics 2019Quote: ... Entry clone plasmids were propagated in One Shot Top10 chemically competent E.coli (Invitrogen) with kanamycin 50 micrograms/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... mCherry-NLS-DEF6 plasmid was establish by inserting annealed NLS oligos (ThermoFisher Scientific) between mCherry and DEF6 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with indicated plasmids using Lipofectamine 2000 (#10696153, Thermo Fisher Scientific).
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... Transfection assays for plasmids were performed using Lipofectamine 2000 (Invitrogen; Carlsbad, CA, US) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Ligated plasmid was used to transform DH5α E.coli cells per manufacturer’s instructions (Invitrogen). Colonies were isolated from LB plates containing 50 µg/ml ampicillin and at least 10 colonies per construct were grown in 100 µl of LB containing ampicillin at 37 °C ON ...
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bioRxiv - Genetics 2019Quote: ... This plasmid was transfected into INS832/13 cells using Lipofectamine 2000 (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Genetics 2019Quote: ... A total of 2 μg plasmid DNA was transfected using Lipofectamine 2000 (Invitrogen). For metabolic labeling ...
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bioRxiv - Microbiology 2019Quote: ... and introduced into the plasmid with restriction enzyme-based techniques (Thermo Scientific, Finland). The ligated plasmid was transformed into E ...
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bioRxiv - Genomics 2020Quote: ... f1 G8PPD plasmid (1.5 μg) were transfected into cells using Lipofectamine 3000 (Invitrogen). At 24 h post G8PPD transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... pCS2nCas9n plasmid and mMessage mMachine SP6 in vitro transcription kits (Thermo Fisher Scientific) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... All plasmids and siRNAs were transfected into different cells using lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Plasmids producing the Sup35NMG protein were based on either pYES2 (Thermo Fisher Scientific) or pCM190 (46 ...
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bioRxiv - Developmental Biology 2019Quote: The amplified products were cloned into the pENTR plasmid (Life Technologies, cat # K240020), digested with Eco RI and Bam HI ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfection was achieved by electroporation with the NEON transfection system (Invitrogen, MPK5000). 36 hours post transfection cells were FACS sorted for Cas9-BFP and 1000-2000 cells seeded for clonal expansion on a 15cm plate ...
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bioRxiv - Genetics 2020Quote: ... and quantified plasmid DNA using Qubit dsDNA HS Assay Kit (Thermo Fisher #Q32854). We digested 10 µg of RCP83 using 10 µL SfiI enzyme (NEB ...
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bioRxiv - Cancer Biology 2020Quote: USP18 cDNA plasmids were transfected with Lipofectamine LTX and Plus (Invitrogen #15338-100) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: The pPvvCY-p230p-gfpLuc plasmid was constructed using MultiSite Gateway cloning system (Invitrogen). attB-flanked 5’and 3’homology arms were obtained by amplifying 800bp regions upstream and downstream of PVVCY_0300700 ...
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Tuning Cell-free Composition Controls the Time-delay, Dynamics, and Productivity of TX-TL ExpressionbioRxiv - Synthetic Biology 2021Quote: ... after which any remaining plasmid DNA was removed via digestion with TurboDNase (Invitrogen). RNA integrity was verified via agarose gel electrophoresis ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: 3 × 104 cells were transfected BRE-Luciferase reporter plasmid using Lipofectamine 2000 (Invitrogen). The cell lysates collected 48 hr after transfection ...
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bioRxiv - Bioengineering 2021Quote: ... pcDNA3.1-Sox2 plasmids were constructed and transfected using Lipofectamine 3000 (Thermo Fisher Scientific).The expression levels of nestin (forward primer ...
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bioRxiv - Neuroscience 2020Quote: ... and 24h after plasmid transfection using Lipofectamine® LTX and Plus reagent (Invitrogen). siRNA sequences used are ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transformed and grown in subcloning efficiency DH5α competent cells (Invitrogen # 18265017) before extraction by Miniprep (QIAGEN #27104 ...