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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Mouse OLFR139 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 0.5 µg of hybrid transposase plasmid and Lipofectamine 2000 transfection reagent (Invitrogen) with a 1:2.5 DNA to transfection reagent ratio.
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bioRxiv - Genetics 2023Quote: ... Confirmed plasmids were transfected into HEK293T cells with lipofectamine 3000 (Invitrogen, L3000001) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... were transfected with TDP-43 or control plasmids using Lipofectamine 2000 (Invitrogen) for 48hr following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... with 3 μg of plasmid DNA in 400 μl Opti-MEM (Gibco), and added to each dish ...
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bioRxiv - Microbiology 2023Quote: ... and then transfected with 3µg of amplicon plasmid using Lipofectamine 2000 (Invitrogen). 24 hours after transfection ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Transfection-ready plasmids were purified from bacterial cultures by maxiprep (Invitrogen, #K20006). Expi293 HEK cells (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmids were transfected into HeLa cells using TurboFect Transfection Reagent (Invitrogen). After 48h ...
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bioRxiv - Developmental Biology 2023Quote: Rescue Plasmids were transfected into relevant cell lines using Lipofectamine 2000 (Thermofisher). Cells with insertions were selected using Puromycin (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... and the constructed transfer plasmid using the Cellfectin II (Thermo Fisher, 10362100) transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... pPAX2 and VSVg plasmids using Lipofectamine® 2000 reagent (Thermo Fisher Scientific) in Opti-MEM™ (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... RPE1 cells were transfected with this plasmid by using Lipofectamine 3000 (Invitrogen). Transfected cells were subjected to puromycin selection after transfection and subsequently seeded as single cells into 96-multiwell plates in order to screen for CEP290 KO clones ...
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bioRxiv - Cell Biology 2023Quote: Bacterial expression plasmids were generated via Gateway cloning (Invitrogen, Carlsbad, CA, USA). The effector sequences of SopB ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and subcloned into pFastBac1 recombination plasmid (Thermo Fisher Scientific, Waltham, MA, US) between BamHI and HindIII restriction sites ...
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bioRxiv - Microbiology 2023Quote: ... The purified plasmid was linearized by Anza 24 MssI restriction enzyme (Invitrogen), purified again ...
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bioRxiv - Biochemistry 2023Quote: ... were transfected with 200 ng psDARPin-expressing plasmids using lipofectamine 3000 (Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and then transfected with 3µg of amplicon plasmid using Lipofectamine 2000 (Invitrogen). 24 hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each plasmid was linearised using restriction enzymes (XbaI + KpnI, Thermo Scientific FastDigest), and 10 µg of DNA was used for each transformation ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter-containing plasmid DNA was digested with FastDigest MvaI (Thermo Scientific) and used as template for in vitro transcription of tRNA using the AmpliScribe T7-Flash Transcription Kit for 4 h at 42°C (see Table S3) ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid pZA31-mCherry-TnpB was designed using Vector NTI software (Life Technologies) and synthesized de novo by GENEWIZ Gene Synthesis Services (GENEWIZ ...
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bioRxiv - Neuroscience 2023Quote: ... transfection of the scFv expression plasmids was carried out using 293fectin (ThermoFisher) following vendor’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products and plasmids were restricted with FastDigest BshTI (Thermo Fisher, FD1464) and NotI (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids at 4:1 ratio (by mass) using Lipofectamine 2000 (Invitrogen, 11668019) in Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... Synthetic genes were ordered from GeneArt and plasmids purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng pTI-ARE-LUC reporter plasmid in 10 µL OptiMEM (Gibco) was transected into each well using Fugene HD at a dilution of 1 µg plasmid DNA:4 µL Fugene ...
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bioRxiv - Cell Biology 2023Quote: ... The final plasmid construct was generated by MultiSite Gateway® cloning (Invitrogen). The strain expressing HIS-1/H4-GFP was made using the MosSCI technique (42) ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were cloned into the pENTR/D-TOPO plasmid vector (Invitrogen) to obtain pYC19 (pENTR-AGB1.1 ...
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bioRxiv - Biophysics 2024Quote: ... cells were transfected with 1 μg BacMam plasmids using Lipofectamine 3000 (Invitrogen) in Opti-MEM media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids (2 µg) were delivered using Lipofectamine® 3000 (Invitrogen, L3000-001) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen, cat. no. L3000150) according to the manufacturer’s protocol 12 hours post plating ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were mixed in the above ratio in Turbofect (Invitrogen, USA), incubated at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and 6µg of vector plasmid in 1.5ml of Opti-MEM (Fisher Scientific) and 45µL of TransIT-LT1 (Mirus Bio ...
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bioRxiv - Biochemistry 2024Quote: ... The linear mutagenized plasmids were then phosphorylated using T4 polynucleotide kinase (Invitrogen) prior to ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the PureLinkTM HiPure Plasmid Filter Maxiprep kit (Invitrogen) and equal sgRNA coverage within the library was confirmed by sequencing.
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bioRxiv - Biochemistry 2024Quote: ... Plasmid DNA (100 ng per well) was transfected using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific, Lafayette, CO, USA). Natural transformation was performed according to the procedure described in (27).
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bioRxiv - Microbiology 2024Quote: ... The furin fragment from this plasmid was subcloned into pcDNA3.1(–) (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Genomics 2020Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... A total of 21,981 mouse genes were included in the Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) and all steps were performed at the McGill University and Génome Québec Innovation Centre (Montréal ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse anti-human CD20 APC-H7 and mouse anti-human CD73 PE were from e-Bioscience (Thermo Fisher Scientific). Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with a mouse primary antibody (DSHB 1D4B) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: TNF-α released by mouse peritoneal macrophages was measured by a Mouse TNF-α ELISA Kit (Invitrogen, BMS607-3TEN) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
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bioRxiv - Genomics 2022Quote: Mouse ESCs (mESCs) were cultured in mouse ESC media that contains DMEM (Dulbecco’s modified Eagle’s medium) (Thermo Fisher Scientific) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...