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Citations for Thermo Fisher :
3001 - 3050 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by the addition of 25 mM N-ethylmaleimide (NEM) (Thermo Scientific, Rockford, IL USA) for 20 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Pre-cleared samples were then incubated with Streptavidin (SA)-Agarose (Thermo Scientific, Rockford IL USA) and mixed overnight at 4°C ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: Samples were analyzed on Orbitrap Fusion Lumos mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and then stained with combinations of Foxp3 (FJK-16s) and IL-13 (eBio13A) from Invitrogen and IL-4 (11B11 ...
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bioRxiv - Immunology 2020Quote: CRP and IL-6 were measured using a high sensitivity ELISA (Life Technologies, Carlsbad CA). Plasma soluble CD14 (sCD14 ...
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bioRxiv - Molecular Biology 2021Quote: ... which are pre-treated with 0.1 N sodium hydroxide (cat. SS255-1, Fisher Scientific, IL), 0.5% 3-aminopropyltrimethoxysilane (cat no ...
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bioRxiv - Cell Biology 2022Quote: ... The AAV titer was measured by qPCR using TaqMan (Thermo Fisher Scientific, Rockford, IL, USA) and reported as genome copies (GC)/ml ...
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bioRxiv - Genomics 2022Quote: ... RNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL, USA), and RNA integrity by electrophoresis and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10 % v/v heat inactivated fetal bovine serum (Thermo Fisher Scientific, IL, USA). The culture was incubated for three days at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoreactivity was detected with Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA). ImageJ (Scion Corporation ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... followed by development with chemiluminescent reagents (SuperSignal West Pico PLUS, Thermo Scientific, Rockford, IL, USA) and bands intensities were determined by densitometry scanning using the ImageJ software (National Institutes of Health ...
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bioRxiv - Bioengineering 2022Quote: ... Micro BCA and Pierce BCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). PEG-DET polymer was synthesized by aminolysis of PEG-poly(β-benzyl L-aspartate ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-21 cDNA transcript sequences were amplified by TaqMan probe based qPCR reactions (ThermoFisher, 4331182) using the Applied Biosystems TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: Sandwich ELISA was used to establish plasma concentrations of IL-18 (Invitrogen, Thermofisher, BMS618-3) and ferritin (Abcam ...
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bioRxiv - Immunology 2024Quote: Sandwich ELISA was used to establish plasma concentrations of IL-18 (Invitrogen, Thermofisher, BMS618-3) and ferritin (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Whole-cell protein lysates were prepared in M-PER buffer (Thermo Scientific, Rockford, IL, USA) with 1x Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were mounted on gelatin-coated glass slides (SuperFrost Plus, ThermoFisher Scientific, IL, US), rehydrated ...
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bioRxiv - Neuroscience 2023Quote: ... CA) and Supersignal West pico PLUS Chemiluminescent Substrate was purchased from Thermo Scientific (Rockford, IL). Aurum Total RNA Mini Kit ...
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bioRxiv - Immunology 2023Quote: ... Enzyme-linked immunosorbent assays (ELISA) were performed to detect IL-1β (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Scientific; Rockford, IL). Equal amounts of lysed extracts were loaded on a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5) added with a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, IL, USA). Protein samples were separated by 10% SDS-polyacrylamide gel electrophoresis (20 µg/lane) ...
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bioRxiv - Microbiology 2023Quote: IL-33 (residues 112–270) and its R169A mutant were cloned into vector pET28a (Invitrogen) and were expressed with a TEV protease cleavable N-terminal His6 tag ...
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bioRxiv - Immunology 2023Quote: ... Enzyme-linked immunosorbent assays (ELISA) were performed to detect IL-1β (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed with Pierce Radioimmunoprecipitation (RIPA) buffer (cat. no. 89901, Thermo Scientific, Rockford, IL) with protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Poland) and by Western blotting using Pierce High Sensitivity-streptavidin-HRP (Thermo Scientific, Rockford, IL) diluted 1:40,000 in 3% BSA/TBST.
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bioRxiv - Immunology 2023Quote: ... in the presence of 250U Pierce Universal Nuclease for Cell Lysis (ThermoFisher Scientific, Rockford, IL), cells counted on Beckman Vi-Cell XR Cell Counter ...
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bioRxiv - Cell Biology 2023Quote: ... IL) were cultured in Dulbecco’s minimum essential medium (DMEM) high glucose with 10% FBS (Thermofisher) and 100 μg/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 (PNA Biosciences Cas9 Protein with NLS, NC1279639, Fisher Scientific, LLC, Chicago, IL, USA) were injected into 240 embryos (240 separate embryos per gRNA ...
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bioRxiv - Immunology 2023Quote: Ectocervical biopsies were fixed for 48 hours in 4% formaldehyde (Thermo Fisher Scientific, Rockford, IL) and paraffin-embedded to generate 5 μm sections used for hematoxylin and eosin (H&E ...
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bioRxiv - Genomics 2023Quote: ... Gels were stained with the GelCode® Blue stain reagent (Thermo Scientific, Rockford, IL, USA) after fixation using 50% methanol (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The bands were visualized using west pico chemiluminescent kit (#34580, Thermo Fisher Scientific, Rockford, IL) under chemi-doc touch imaging system ...
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bioRxiv - Immunology 2023Quote: ... Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22) from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2023Quote: ... IL-1R2 concentrations were determined in culture supernatants by an ELISA assay (EHIL1R2, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) for 24hr [45 ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific binding was blocked by Starting Block T20 PBS Blocking Buffer (Thermo Scientific, Rockford, IL) for 1 hour followed by primary antibodies targeting EV membrane protein ...
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bioRxiv - Microbiology 2023Quote: ... EV protein content was measured using Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined employing the PierceTM BCA protein quantification kit (Thermo Scientific, IL, USA). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following manufacturer’s instructions (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Biochemistry 2024Quote: Pierce LC-MS grade water and formic acid were purchased from Thermo Scientific (Rockford, IL). LC-MS grade acetonitrile and 2-propanol were purchased from Honeywell (Charlotte ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted with a coverslip using ProLong Gold antifade mounting media (Thermo Fisher, Rockford, IL). Images were acquired using a Nikon A1 Confocal microscope (Nikon TE2000 inverted microscope provided by the UAB Microscopy Core).
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bioRxiv - Plant Biology 2019Quote: ... pEF1-MCS-3Myc [BamHI-NotI-3Myc-stop fragment was inserted between KpnI and XbaI sites of pEF1/myc-His B vector (Invitrogen)] was generated ...
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bioRxiv - Microbiology 2020Quote: The parB genes were recombined into a Gateway-compatible destination vector pET-His-MBP-TEV-DEST 56 via an LR recombination reaction (ThermoFisher). For LR recombination reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... was modified by replacement of the SV40 promoter by the Drosophila actin promoter from the pAct5.1/V5-His C vector (Thermo Scientific), and the firefly luciferase coding sequence by the Renilla luciferase (RLuc ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...