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Citations for Thermo Fisher :
2951 - 3000 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... M-PER was supplemented with protease and phosphatase inhibitors (PPI; Thermo Scientific, Rockford, IL, USA). After aggressively vortexing these cells for 3x 1-minute increments ...
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bioRxiv - Cancer Biology 2021Quote: ... they were resuspended in mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL, USA) supplemented with protease and phosphatase inhibitor (PPI ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were immersed in PBS with protease inhibitors (Pierce 88266, Fisher Scientific, Rockford, IL, USA) to minimize post-mortem degradation ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... DNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL, USA), DNA quantity with the DNA High Sensitivity kit on a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were mounted on gelatin-coated glass slides (SuperFrost Plus, Thermo Fisher Scientific, IL) and stained for 20 min with a cresyl-violet solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific, Rockford, IL, USA). 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: WI-26 cells were plated in a Lab-Tek chamber slide (Nalgen Nunc International, IL) at 3 × 104 cells per well and incubated overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were revealed using Pierce Silver Staining kit (Thermo Fisher Scientific, Rockford, IL, USA) according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2019Quote: ... loaded onto a 4-12% NuPage Bis-Tris gels (Thermo Fisher Scientific, Rockford, IL, USA). The proteins were revealed using Pierce Silver Staining kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... and then incubated with 10 μg/cm2 MA(PEG)24 (Thermo Scientific, Rockford, IL, USA) for two hours ...
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bioRxiv - Molecular Biology 2019Quote: ... secondary antibodies were visualized with an enhanced chemiluminescence detection kit (Thermo Scientific, Rockford, IL, USA). The band intensities were quantified using Gene Tools software (Sygene ...
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bioRxiv - Microbiology 2021Quote: Cell culture supernatant was harvested and used to carry out ELISA for IL-1β (Invitrogen) and IL-18 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: WI-26 cells were plated in a Lab-Tek chamber slide (Nalgen Nunc International, IL) at 3 × 104 cells per well and incubated overnight ...
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bioRxiv - Physiology 2021Quote: ... and developed with SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). Protein extracts were mixed with 6X SDS sample buffer (Tris pH 6.8 ...
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bioRxiv - Cell Biology 2020Quote: ... Metabolite levels were normalized to total protein as determined by BCA (Thermo Scientific, Rockford, IL) and fold changes compared to day 4 control samples were calculated.
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bioRxiv - Cancer Biology 2021Quote: ... The expression of recombinant IL-18 was induced by 0.5 mM IPTG (Thermo-Fisher Scientific) for 8 h at 37 °C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Total protein in the lysate was quantified via a BCA assay (Thermo Scientific, Rockford, IL). A 4 to 20% gradient Tris-Glycine gel was loaded with 30 μg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... using samples diluted to 1:1000 while interleukin (IL)-6 (Thermo Fisher Scientific, Cat. #BMS625) was evaluated using samples diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Cancer Biology 2022Quote: ... and secondary goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (Thermo Fisher Scientific, IL). To detect cell-surface HER2 expression by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... were dissected and fixed in Richard-Allan Scientific™ Buffered Zinc Formalin (ThermoFisher, Rockford, IL) overnight (∼15 hr ...
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bioRxiv - Immunology 2020Quote: ... and CD4–PE-Cy5.5 (clone S3.5) and IL-17A–PE (clone eBio64DEC17) from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... The immunopositive spots were visualized by using chemiluminescent reagent (Thermo Scientific Pierce, Rockford, IL, USA) as directed by manufacturer ...
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bioRxiv - Systems Biology 2021Quote: ... Liquid chromatography separation was accomplished on an Ultimate 3000 RSLCnano (Thermo Scientific, Rockford, IL, USA). Samples were loaded directly onto Acclaim Pepmap® C18 (75 μm × 150 mm ...
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bioRxiv - Cancer Biology 2022Quote: ... protein concentration was determined using Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Rockford, IL) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from CD14+ cells (MagJET RNA kit, Thermo Fisher Scientific, Rockford, IL) using the protocol adapted for tissue (KingFisher Duo machine ...
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bioRxiv - Synthetic Biology 2019Quote: ... The resuspended cultures were distributed into 15 mL polypropylene centrifuge tubes (Thermo Scientific, IL, USA) with a working volume of 5 mL and grown for 24 hour (h ...
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bioRxiv - Biophysics 2019Quote: ... and 1X protease inhibitor added prior to lysis (Thermo Fisher Scientific, Pierce, Rockfrod IL USA). Lysed cells were centrifuged at 10,000X g for 7 min and supernatant was transferred onto packed streptavidin beads and incubated with end-over-end mixing for 1 hr at RT ...
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bioRxiv - Cell Biology 2019Quote: ... were from Proteintech Group Inc (Chicago, IL). pAb against claudin-5 (cat. no. 34-1600) was from Invitrogen and mAb against β-actin (cat ...
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bioRxiv - Bioengineering 2019Quote: ... HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific; Rockford, IL) and the reaction was stopped with 2N sulfuric acid ...
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bioRxiv - Plant Biology 2019Quote: ... using the SuperSignal West Pico detection system (Pierce-Thermo Fisher Scientific Inc., Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... Then cell supernatant was harvested and the cytokine IL-1β (Catalog # 88-7013-22, Invitrogen) and TNFα (Catalog#88-7324-88 ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was visualized by enhanced chemiluminescence kit (Thermo Fisher Scientific Inc., Rockford, IL, USA). The intensity of the bands was measured with a scanning densitometer (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Fractionation was performed with Subcellular Protein Fractionation Kit from Thermo Scientific (#78840, Rockford, IL, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the plates were emptied and blocked with 300 µL blocking buffer (Thermo Scientific, IL, USA) at RT for 1 hrs ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNF-α levels were determined by ELISA kit (ThermoFisher, for MCP-1) and (Affymetrix eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, IL). Cleared lysates were supplemented with protein sample buffer ...
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bioRxiv - Pathology 2021Quote: ... and the fraction was subsequently passed through Detoxi-gel (Thermo Fisher Scientific, Rockford, IL, USA) to remove LPS ...
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bioRxiv - Systems Biology 2020Quote: ... Digested samples were desalted with Pierce Peptide Desalting Spin Columns (Thermo Fisher Scientific, IL, USA). Phosphopeptide enrichment was subsequently performed using PolyMac spin tips (Tymora Analytical ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants were combined and 300 μL of neutravidin agarose resin (Thermo Fisher Scientific, Rockland, IL) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Protein quantitation was performed using bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific, Rockford, IL); 100 µg of each sample was used for TMT labeling.
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bioRxiv - Immunology 2020Quote: ... IL-17 and IFN-γ were measured by ELISA Ready-Set-Go kits from Thermofisher and culture supernatants were tested at different dilutions according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Commercially available direct ELISA kits were performed to determine bovine IL-1β (ESS0027 Invitrogen®) and IL-8 (3114-1A-6 MABTECH ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations were determined by the BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL). Equal amounts of protein (10-50 µg ...
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bioRxiv - Systems Biology 2020Quote: ... Immunoreactive moieties were detected by chemifluorescent detection (Pierce ECL Plus, Thermo Fisher Scientific, Rockford, IL) of horseradish peroxidase-conjugated (HRP ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed and 100 µl of TMB substrate (ThermoFisher Scientific, Rockford, IL, USA) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then subjected to qPCR using PowerUp SYBR Green Master Mix (ThermoFisher, Rockford, IL) and the following primer sets detecting three transcripts ...
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bioRxiv - Physiology 2021Quote: ... The samples were acidified with 50% trifluoroacetic acid (TFA, Thermo Fisher Scientific, Rockford, IL, USA) until the pH of the samples was lesser than 2 ...
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bioRxiv - Developmental Biology 2021Quote: Stock solution of doxycycline hyclate (dox) (446060250, Acros Organics, Fisher Scientific, Hanover Park, IL, USA) was made up to final concentration of 1 mg/mL in filter sterilized water ...