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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA loading buffer was added and 10 mL of the reactions were electrophoresed on 6% DNA Retardation gels (Invitrogen) at 130 V for 60 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Vero-6 cells (ATCC CRL-1586) were maintained in DMEM medium (Gibco, 4.5 g/L glucose, w/o pyruvate) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Molecular Biology 2021Quote: ... ESCs were transfected in a single well of a 6-well plate with 0.5μg Cas9 guide plasmid and 2μg targeting construct plasmid using Lipofectamine 3000 (Thermo Fisher Scientific) according to manufacturer’s guidelines ...
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bioRxiv - Zoology 2020Quote: Xela DS2 (passage 74) and Xela VS2 (passage 75) cells were seeded in 6-well plates (BioLite, Thermo Scientific) at cell densities of 300,000 cells/well in 1 mL of complete medium and allowed to adhere overnight at 26°C ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Microbiology 2019Quote: 293T cells in 6-well tissue culture plates were transfected using Lipofectamine2000 according to manufacturer’s instruction (Thermo Fisher Scientific). For scaleup transient transfection of 293S GnTI- cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 6-12 µg anti-HA antibodies (i.e., 12CA5, University of Chicago Monoclonal Antibody Facility) or 6 µg anti-V5 antibodies (R960-25, Invitrogen) per reaction were first conjugated to protein A sepharose beads in a 50% slurry that was first washed in IPP150 ...
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bioRxiv - Cell Biology 2019Quote: ... and oligonucleotides targeting genes of interest (IDT) in triplicate and run on QuantStudio 6 Flex (Applied Biosystems Cat# 4485694) using protocols according to the mastermix manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, part of Life Technologies Corporation, CA) according to the manufacturer’s recommendations using a melt protocol in 0.05-degree/sec increments from 25°C to 95 °C ...
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bioRxiv - Genetics 2019Quote: ... 2.4×104 cells were seeded per well of a 6-well cell culture plate containing 4ml of DMEM (Gibco) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670, 0.001% Brij35 ThermoFisher, #28316 ...
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bioRxiv - Genetics 2019Quote: ... about 6 males and 10 female flies were flipped onto a new flask (Fisher Scientific, Cat. No.:AS-355) of food and allowed to mate at 25°C and lay eggs ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions while still in the stem cell state ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cells were then seeded onto Matrigel-coated 6-well plates containing Essential 8™ Medium (ThermoFisher #A15169-01) with added Y-27632 for recovery following nucleofection ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 100×103 cells / well cultured in 6 well plates were transfected with 0.8 μg each plasmid DNA using Lipofectamin2000 following the manufacturer’s protocol (ThermoFisher Scientific) and analysed 48 hours later.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... insect cells (cell density at 5 x 105 cells/mL) using 6 μL of CellFectin reagent and 100μL of SFM II media with antibiotics (penstrep, 0.5X) (Gibco™) and allowed to grow for 1 hour at 28°C ...
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bioRxiv - Neuroscience 2020Quote: ... L4-6 ganglia were isolated from adult male Sprague Dawley rats (175-250 g) and dissociated with collagenase (Invitrogen). Cells were washed and then plated with DMEM/F12 (Cellgro ...
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Immunology 2021Quote: ... kidneys were wax-embedded and sliced into 6 μm sections onto SuperFrost Plus™ adhesion slides (Thermo Fisher Scientific). Slides were air-dried O/N at RT and ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2019Quote: ... hippocampi were dissected from E18 Sprague-Dawley rat embryos or E17 C57BL/6 mice embryos and treated with 0.25% trypsin in HBSS (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Cells were reverse-transfected (transfected while plating) in 6-well plates (3.5 -5 × 105 cells per well) using Lipofectamine RNAimax transfection reagent (Invitrogen), following standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal or cortical neurons were plated at 17,000 cells/well (96-well plate) or 1,000,000 cells/well (6-well plate) in neuron media (Neurobasal medium (ThermoFisher 21103049) supplemented with B27 (ThermoFisher 17504044) ...
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bioRxiv - Neuroscience 2021Quote: ... Retinas were then dissected in DPBS and placed on a culture insert (Millicell) in a 6-well plate (Falcon) containing 1.3ml of equilibrated medium: DMEM (supplemented with glutaMAX; Gibco) with 10% FBS (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.6 µg KV3.1b plasmid and 0.1 µg eGPF were transfected into HEK-293 cells using Lipofectamine 2000 (Invitrogen Corporation), and recordings were performed after 3 days of incubation at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: DRGN and retinal cells were washed with PBS and total RNA (n = 6-9 wells/treatment) was extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen). Appropriate primers were used to validate each gene of interest using the specific primers (Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The nucleofected cells were plated on a well of a 6 well plate coated with Geltrex matrix (Life Technologies) and transitioned to Essential 8 media (Life Technologies)1 ...
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bioRxiv - Molecular Biology 2019Quote: ... After approximately 6 hours the cells were washed with phosphate-buffered saline (PBS) and detached with TrypLE (ThermoFisher Scientific). Then ...
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bioRxiv - Cell Biology 2021Quote: 300,000 cells/well were seeded into duplicate wells of 6-well plates (Thermo Fisher Scientific, Cat# 07-200-83) in 2 mL medium ...
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bioRxiv - Cancer Biology 2019Quote: ... whose NBD-amino group is placed at carbon 6 and can therefore not be phosphorylated by HKs34 (Thermo Fisher). The cells were then washed with ice-cold phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were stably transfected into Fischer Rat Thyroid (FRT) cells in 6-well plates using lipofectamine LTX (Thermo Fisher) and OptiMEM (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: The qPCR was conducted in duplicate using 384-well plates on a QuantStudio™ 6 cycler (Thermo Fisher Scientific). The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... The qPCR was performed by QuantStudio™ 6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). Elongation Factor 1α (CqEF1α)(XM_021888526.1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR amplification was performed on QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) with the following conditions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Ribosome was depleted in 6 µg of RNA from each pool using the Dynabeads mRNA purification kit (Ambion, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then washed twice with PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Scientific #D1306) diluted 1:200 in 0.5% BSA in PBS ...
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bioRxiv - Molecular Biology 2021Quote: Transient expression of DNA plasmids was carried out in 6 well plates by reverse transfection using Lipofectamine LTX (Invitrogen). Briefly ...