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Citations for Thermo Fisher :
2951 - 3000 of 3050 citations for GRO beta CXCL2 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in 0.1M PBS and incubated in goat anti-rat Alexa Fluor 568 secondary antibody (1:500, catalog #A11077, Thermo Fisher Scientific; RRID: AB_2534121) for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... haemoglobin RNA was removed from 1 µg of RNA extracted from blood samples using the GLOBINclear-Mouse/Rat Globin mRNA Removal Kit (Thermo Fisher Scientific; AM1981) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times in PBS and incubated with secondary antibody (Alexa-Fluor-647 goat anti-rat, Thermo Fisher, 1:200 dilution) for 1 hour at room temperature ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rat anti-Tbr2 (1:500, Invitrogen 14-4875-80), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2022Quote: ... brains were fixed for 14 min in freshly prepared 4% paraformaldehyde and incubated overnight in rat anti-CD8 (1:50, Thermo Fisher #MA5-17594) and mouse nc82 (1:40 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were used with goat anti-Rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo fisher, SA5-10010, 1:400 dilution) or goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used were goat anti-rat Alexa Fluor 488 or goat anti-mouse Alexa Fluor 596 (Thermo Fisher scientific, 1:1000). The incubation with antibodies was accomplished in a humidified chamber for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Brain slices were then incubated either 4 hours at room temperature or overnight at 4°C in 1.5% Normal Donkey Serum in PBS-T with donkey anti-rat DyLight 550 (ThermoFisher, SA5-10029, 1:1000), donkey anti-goat AlexaFluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... unconjugated primary antibodies were detected with fluorescently-labelled species appropriate secondary antibodies including Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 and Goat Anti-Rat IgG (H+L) Alexa Fluor 647 (ThermoFisher Scientific, Brisbane, Australia). All IF sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey anti-rat 555 secondary antibody (ThermoFisher, A48270, 1:1000), donkey anti-guinea pig 647 secondary antibody (Biotium ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained for 1 h at 37°C with Alexa Fluor 488-labeled chicken anti-rat IgG (Invitrogen, A21470, 1:300) and Alexa Fluor 546-labeled goat anti-mouse IgG (Invitrogen ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with perm buffer and then incubated with 20 μL of perm buffer containing 10% rat serum (ThermoFisher 24-5555-94) for 20 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated overnight at 4°C with the following primary antibodies diluted in 1% Normal Goat Serum: Rat anti-Ki67 conjugated FITC (1:50, Invitrogen, 11-5698-82), Mouse anti-active/pro-caspase3 (1:20 ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from whole heart according to the aforementioned procedure and analyzed by means of GeneChip technology (GeneChip Arrays platform with Clariom™ S Assay, Rat; Thermo Fisher Scientific). Standard quality control steps were included to determine total RNA quality with use of Agilent Bioanalyzer 2100 (RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then blocked with 1% bovine serum albumin in PBS for 1 hour before incubating with rat anti-GFAP (Thermo Fisher, 13-0300) or rabbit anti-cFos (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by secondary antibodies (Alexa Fluor Goat anti-Rat 488 Invitrogen #A-11006, 1:1000 and Alexa Fluor Goat anti-Rabbit 594 Invitrogen #A-11012, 1:1000). Coverslips were mounted on slides with Fluoromount-G mounting media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were washed in PBS-T and incubated with the corresponding anti-rabbit or anti-mouse Alexa Fluor 568-conjugated and anti-rat Alexa Fluor 488-conjugated IgG secondary antibody (all 1:1000, Thermo Fisher Scientific, Waltham, MA) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the secondary antibodies (biotinylated goat anti-rat antibody, Jackson 112-066-072, 1/500; goat anti-rabbit Alexa fluor 635, Life technologies A31577, 1/500) and DAPI (4/1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed three times in PBS-T and were incubated for 2 hr with the secondary antibodies Alexa Fluor® 488 or 546 Goat Anti-Mouse/Rat/Rabbit IgG (Life Technologies; 1:200) and then with DAPI (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 1 hour at room temperature with goat anti-rat Alexa-Fluor 488 conjugate antibody (Molecular Probe, ThermoFisher Scientific Waltham, MA, USA) diluted 1:500 in PBS ...
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bioRxiv - Cell Biology 2020Quote: Cardiomyocytes were isolated from the left ventricle of 1–2-day-old Sprague-Dawley rats following the protocol from an isolation kit (Cellutron Life Technologies, cat#nc-6031). Myocytes were then plated on cover slips coated with laminin (Life tech #23017-015 ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed with PBS-T and then were incubated with Alexa Fluor 488–conjugated donkey anti–rat IgG (1:500; Invitrogen, California, USA; RRID AB_2535794), Alexa Flour 594–conjugated donkey anti–rabbit IgG (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed and incubated with 1 µg/ml Donkey anti-Rat polyclonal Dylight 488-conjugated antibody (Thermo Fisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and subsequently for 16 hours at 4°C in blocking solution containing primary antibodies (anti-A2AR mouse antibody, sc-32261 Santa Cruz Biotechnology, at 1:100 and anti-CD31 rat monoclonal antibody, ER-MP12 Thermo Scientific, 1:20). For detection of A2AR and CD31 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ileum and brain of the sub-chronic IP injected animals as well as the CIE rats was extracted using Trizol (Thermo Fisher, Waltham, MA, USA) and the RNeasy MicroElute RNA extraction kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were flipped onto 60 mm dishes containing 15 DIV rat hippocampal glial cells cultured in Neurobasal plus medium (Gibco Thermo Fisher Scientific, #A3582901) supplemented with 2 mM glutamine and 1x B27TM plus Neuronal supplement (Gibco Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and the secondary antibodies solution (1:75 dilutions of Alexa Fluor 488 goat anti-rat and Alexa Fluor 546 goat anti-mouse [Molecular probes, A11006 & A11030, respectively]), always washing with PBS-T in between ...
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bioRxiv - Microbiology 2021Quote: ... The CD3 (Rat anti-CD3 clone CD3-12, P. Moore, UC-Davis, Davis, CA) and CD20 (Rabbit anti-CD20, Thermo Fisher Scientific, Waltham, MA) primary antibodies were applied for 30 m at 1:25 and 1:400 dilutions ...
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bioRxiv - Neuroscience 2020Quote: Tissue sections from male (n = 3) and female (n = 3) rats were mounted on subbed glass slides (Fisher brand Superfrost Plus, Fisher Scientific, Hampton, NH, USA) and desiccated overnight (~16 h ...
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bioRxiv - Neuroscience 2022Quote: ... from E18 Sprague Dawley rats were thawed and cultured according to the manufacturer’s recommendations in a B-27 Plus Neuronal Culture System (Thermo Fisher Scientific, cat. no. A3653401). The thawing and culturing medium (NB Plus + ...
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bioRxiv - Neuroscience 2019Quote: ... at 4°C overnight, rinsed in PBS (3 times, 5 min each) and amplified by Alexa Fluor-488 donkey anti-rat antibody (Life Technologies, A21208, 1:600). Sections were mounted with Fluoromount containing DRAQ-5 (1:1000).
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bioRxiv - Plant Biology 2021Quote: ... the slides were incubated with secondary goat anti-rabbit or goat anti-rat antibodies conjugated with Alexa Fluor 488 or Alexa 555 (Thermo Fisher, diluted 1:100) in PBS containing 0.01 % acetylated BSA at 37 °C for 2h ...
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bioRxiv - Cell Biology 2019Quote: Py-3Y1-S2 rat fibroblast cells were cultured in Dulbecco’s minimum essential medium (DMEM) containing 5% fetal bovine serum (Gibco, Thermo Fisher Scientific K.K., Tokyo, Japan) in the presence of 5% CO2 at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... rat anti-Vasa (1:100, Developmental Studies Hybridoma Bank, RRID: AB_760351) and rabbit anti-GFP (1:2500, Thermo Fisher, cat# A-11122, RRID: AB_221569). Staining was detected with the following conjugated antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated for two hours at room temperature with goat anti-Rat IgG (H+L) Alexa Fluor 488 secondary antibody (ThermoFisher Scientific, diluted 1:200). Antibodies were diluted in 1×PBS with 5% BSA and incubated in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific, A11006, 1:1000) for CD44 and Alexa Fluor-594 anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Later optimization steps were performed using genomic DNA from SD rats extracted from tail tissue using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, Waltham, MA). HS rat DNA was extracted from spleen tissue using the Agencourt DNAdvance Kit (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed twice with PB then incubated with species-specific secondary antibodies diluted in PBS-T for two hours (goat anti-rat conjugated to Alexa 488; ThermoFisher; #A-11006; 1:500 and goat anti-rabbit conjugated to Alexa 647; ThermoFisher; A-21247; 1:500). After two final rinses in PB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and scanned using the standard Affymetrix procedure (Affymetrix GeneChip® Mouse Genome 430 2.0 Array or Affymetrix GeneChip® Rat Genome 230 2.0 Array). Quality control and low level analysis of raw fluorescence intensities was performed using the affy package (v1.64.0 ...
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bioRxiv - Neuroscience 2023Quote: Sections were washed in PBS three times for 5 min each and incubated in secondary antibodies in PBS (goat or donkey anti-rabbit, anti-mouse, or anti-rat fluorescent antibodies; Invitrogen Alexa Fluor; 1:500) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... and HA were labeled with fluorescent secondary antibodies (donkey anti-rat IgG, 1:200; donkey anti-rabbit IgG, 1:200, Thermo-Fisher A-31573; anti-streptavidin IgG, 1:200, Invitrogen Molecular Probes S-32355), mounted onto glass slides ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were washed 3 times with PBS for 5 min each and incubated with secondary antibodies anti-mouse Alexa fluor 594 and anti-rat Alexa fluor 488 (1:250, Invitrogen #A11005 and #A11006, respectively) in blocking buffer for 1h at RT in a dark humid chamber ...