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Citations for Thermo Fisher :
2901 - 2950 of 3050 citations for GRO beta CXCL2 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
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bioRxiv - Biochemistry 2024Quote: ... The following secondary antibodies were used at a 1:1000 dilution for cultured cells: donkey anti-rat Alexa Fluor 488 (Invitrogen A-21208) and donkey anti-mouse Alexa Fluor 488 (Invitrogen A-21202) ...
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bioRxiv - Immunology 2024Quote: ... for one hour and incubated overnight with the primary antibodies (rabbit-anti-Iba-1, 1:200; Wako, USA; rat-anti- mCherry, 1:2000, Thermo Fisher Scientific). After washing in PBS ...
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bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645; Invitrogen A10043 Lot 753765) at 1:500 in PBS+0.3% triton X-100 overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and rat pAbs horseradish peroxidase-conjugated (HRP-Ab) (#111-035-003, #115-035-003, Jackson Immunoresearch Laboratories; #31470, Invitrogen; 1:5000 dilution).
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked with 1% normal goat serum (NGS) in 1X PBS for 1 h followed by incubation with primary antibodies [rat anti-mCherry (Thermo Fisher, #M11217)] overnight at 4°C for standard immunohistochemistry.
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bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 nM of either ON-TARGETplus Non-targeting Control siRNA Pool (Dharmacon #D-001810-10-05) or On-TARGETplus Smartpool siRNA targeting rat ACLY protein (Dharmacon #L-089773-02-0005) using lipofectamine RNAimax (Thermofisher Scientific #13778075), as per manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin C (primary: mAb 14D12, Abcam catalog no. ab106046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α- IgM (mAb II/41 ...
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bioRxiv - Neuroscience 2021Quote: ... mAb24B10 (DSHB, 1:50), anti-OLLAS (Funakoshi, 1:200) and goat anti-rat IgG(H+L) Alexa Flour 488 conjugate (Life Technologies, 1:400).
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with PBS for 3 × 10 minutes prior to incubation overnight at 4°C with the Alexa secondary cocktail: Alexa Fluor 555 dye-conjugated goat anti-rat (1:1000, Invitrogen, Eugene, OR, USA) and Alexa Fluor 488 dye-conjugated goat anti-mouse (1:600 ...
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bioRxiv - Developmental Biology 2019Quote: ... for 1 hour at room temperature and Alexa647-conjugated goat-anti rabbit or goat anti-rat secondary antibodies highly cross-adsorbed against IgG from other species (ThermoFisher Scientific, 1:200) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated with secondary antibodies for 1 hour at room temperature [donkey Alexa 488 anti-rat (1:1000, Invitrogen, Carlsbad, CA, #A21208), goat Alexa 555 anti-rabbit (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were incubating with the primary antibody diluted at 1/100 (anti-PI4Kα1) and with secondary antibody diluted at 1/300 (anti Rat IgG, alexa Fluor 488 conjugate, InVitrogen-Molecular Probes, #A-21210) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Cell Biology 2021Quote: ... Primary neuronal cultures from cortices of 2-day-old rats were prepared and maintained at 37 °C with a 5% CO2-containing atmosphere in neurobasal medium (Life Technologies, 21103-049) supplemented with 2% B27 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin A (primary: mAb 8G6, Hycult catalog no. HM1035; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-mannose binding lectin C (primary ...
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bioRxiv - Neuroscience 2021Quote: ... coverslips were flipped onto 60 mm dishes containing 15 DIV rat hippocampal glial cells cultured in Neurobasal plus medium (Gibco Thermo Fisher Scientific, #A3582901) supplemented with 2 mM glutamine and 1x B27TM plus Neuronal supplement (Gibco Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated in primary antibody (chicken anti-GFP, Aves labs GFP-1010, 1/500; goat anti-PDGFRA, R&D Systems AF1062, 1/200; rat anti-SOX2, ThermoFisher 14-9811-82 ...
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bioRxiv - Neuroscience 2022Quote: ... rat neurons were seeded in eight-well Lab-Tek II chambered cover glasses (German #1.5 borosilicate glass; Thermo Fisher Scientific, cat. no. 155409) at a density of 100,000–110,000 neurons per well ...
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bioRxiv - Cancer Biology 2020Quote: ... After incubation with secondary antibodies (Alexa-Fluor 594 goat antirabbit, Invitrogen Cat # A-11012 and Alexa-Fluor 488 goat anti-rat, Invitrogen Cat # A-11006) diluted 1:1000 in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: REF52 cells (an immortal line of post-crisis Fischer rat embryo cells were grown in Minimum Essential Medium α (MEM α) (Gibco/Invitrogen) supplemented with 10% bovine growth medium (BGS ...
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bioRxiv - Microbiology 2020Quote: ... 474-1506), anti-mouse at 1:3,000 (Insight Biotechnology, Cat no. 474-1506) or anti-rat at 1:4000 (Life technologies, Cat. no 629520). The blots were visualised using ECL Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The slides were washed three times for 10 min with 0.033% Triton X-100 and incubated with an anti-rat antibody conjugated with Alexa Fluor® 488 (1:1000 dilution, Thermo Fisher Scientific) in blocking buffer for 1 hr at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific; A18869, Thermo Fisher Scientific) for monoclonal antibodies ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated and unconjugated primary antibodies were detected by incubating with Streptavidin-PE (Pharmingen, 534061) and Rat anti-mouse IgG2a antibody (Invitrogen, 17-4210-80) respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa 488-conjugated donkey anti-goat and Alexa 488-conjugated donkey anti-rat antibodies were purchased from Life Technologies (Grand Island, NY, USA).
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bioRxiv - Physiology 2021Quote: ... and incubated with secondary antibody (conjugated goat anti-rat Alexa fluoro 546, 1:1,000 dilution, A11081; Invitrogen by ThermoFisher Scientific; Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
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bioRxiv - Cell Biology 2021Quote: ... the cell surface localized LAMP-1 protein was detected using immunolabeling with rat monoclonal anti-LAMP-1 antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific; RRID:AB_657536). The cells were moved to fresh media (containing chemical treatments ...
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated with a secondary antibody conjugated with a biotin or fluorescence label (for immunofluorescence) or biotin for immunohistochemistry (for CD31, PEG10 and CD45, 1:500 goat anti-rat IgG conjugated with Cy3 (Thermo Fisher Scientific, A10522); for Cytokeratin ...
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bioRxiv - Neuroscience 2020Quote: ... A further incubation of sections commenced in a secondary antibody solution containing 1:500 donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) in 0.1 M TBS for 24 hours at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibodies include: Alexa Fluor® 647donkey anti-rabbit IgG (A31573) and Alexa Fluor® 488 donkey anti-rat IgG (A21208) purchased from Life Technologies
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bioRxiv - Immunology 2022Quote: ... sections were with the respective fluorochrome-conjugated secondary antibodies (goat anti-mouse/anti-rabbit/anti-rat, streptavidin etc, conjugated to AlexaFluor 488, 594, 633, 647, all purchased from ThermoFisher Scientific; 1:500) for 2 hours at room temperature in 5% normal goat serum and 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Organoids were then washed three times with 1x PBS and incubated with Alexa Fluor 647 Goat anti-Rat IgG secondary antibody (Invitrogen, A-21247, RRID:AB_141778) diluted 1:400 in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: The following secondary antibodies were used at a 1:1000 dilution for tissue and cell staining: donkey anti-rat Alexa Fluor 594 (Thermo Scientific A-21209), goat anti-rat Alexa Fluor 647 (Thermo Scientific A-21247) ...
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bioRxiv - Neuroscience 2022Quote: ... were stained for hematoxylin and eosin or immunostained for glial fibrillary acidic protein (GFAP; rat-anti-GFAP; #13-0300 Thermo Fisher Scientific; RRID:AB_2532994), ionizing calcium binding adaptor protein 1 (IBA1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked using 2.5% fish gelatin in a solution of 0.05% Tween20 in PBS followed by incubation with primary anti-Ki67 (ThermoFisher, rat, 14-5698-82), incubated over-night at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from dissected ipsilateral and contralateral vMB from AAV-TYR- injected rats using mirVana PARIS RNA and Native Protein Purification Kit (Thermo Fisher Scientific # AM1556). RNA concentration was determined using a NanoDrop ND-1000 Spectrophotometer ...
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bioRxiv - Neuroscience 2022Quote: Dried formaldehyde fixed 50mM floating sections of uninjured rat spinal cord were rehydrated in PBS and then treated with 0.25% trypsin EDTA solution (Gibco cat# 25-200-056) or 20mg/mL proteinase K (recombinant PCR grade ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue sections were washed three times with 0.01M PBS and incubated with goat anti-rat Alexa Fluor 488 (1:1000; Invitrogen, cat no. A-11006) and donkey anti-rabbit Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells and rat neurons were plated on 18 mm coverslips that had been coated with poly-D-lysine (Thermo Fisher Scientific, ICN10269491) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hr under room temperature followed by an 1-hr incubation of an alexa 488 goat anti-rat secondary antibody (Thermo Fisher, A-11006). The slides were mounted with VECTASHIELD Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Neuroscience 2024Quote: ... Biotin-GCO AGC TOG ATO TTG OGC OC (O = C-phosphorthioate) directed against a unique region of the rat mRNA was synthesized by Invitrogen (Waltham, MA, USA). The corresponding GenBank accession number and ODN position within the mRNA sequence are NM_017171.2 and 420-439 ...
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bioRxiv - Developmental Biology 2024Quote: ... culture conditions were established using a mix of 50% Janvier Labs Rat Serum Sprague Dawley RjHan SD (male only) and 50% DMEM FluoroBrite (Thermo Fisher Scientific, A1896701) with incubation at 37°C and a 7% CO2 concentration ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were labelled for 2 h at room temperature with secondary antibodies (α-rat Alexa fluor 488, α-rabbit Alexa fluor 568, α-rabbit Alexa fluor 594 - Invitrogen) then washed again 3 times in PBS ...