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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gels were then rinsed one time in deionized water and placed into an iBlot 2 mini transfer stack (Thermo Fisher Scientific). The transfer stack was loaded onto the iBlot 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for one hour at room temperature with a blocking reagent 2% bovine serum albumin (BSA; Fisher Scientific) in PBS followed by staining with primary antibodies ...
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bioRxiv - Pathology 2023Quote: ... which was previously labeled at its 5’ end with one of four specific fluorescent dyes (6-FAM®, NED®, PETTM and VICTM; Thermo Fisher Scientific, Waltham, MA, U.S.A) (Table 2) ...
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bioRxiv - Cell Biology 2024Quote: ... a premium microscope slide (Fisher-finest, 3″ × 1″ × 1 mm; Thermo Fisher Scientific, 12-544-1) or chambered coverglass (1 well ...
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bioRxiv - Neuroscience 2020Quote: ... Iba-1 (1:1000, Wako) and COX-2 (1:1000, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Immunology 2023Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Plant Biology 2021Quote: ... these were extracted and dialyzed against 25 mM Tris pH 7.5 at 4 °C and measured using a Nanodrop One (Thermo Fisher Scientific, Waltham, MA). Particles for cryo-EM were dialyzed against 20 mM NaOAc ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were incubated at 4°C for one day in PBT with 0.002% Streptavidin conjugated to Alexa Fluor 633 (ThermoFisher Scientific, Waltham, MA, USA), then washed two times in PBT and two times in PB ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then washed twice with Blocking One (Nacalai Tesque, 03953-95) and incubated overnight at 4 °C with primary antibodies diluted in 10% goat serum (Thermo Fisher Scientific, 16210064)/Blocking One ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We used para-chlorophenylalanine (PCPA, a.k.a. DL-4 Chlorophenylalanine, fenclonine) (CAS: 7424-00-2, ThermoFischer, Acros Organics, Geel, Belgium; stored at 4°C), an inhibitor of the enzyme tryptophan hydroxylase (TPH) ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... After incubation for 1h with the appropriate secondary antibodies (Alexa Fluor 488 or 594 (Thermo Fisher)) ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 1h on ice with or without RNase (10μg/mL RNase A) (Thermo Fisher). Lysate was centrifuged at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1h followed by incubation with goat α-rabbit Alexa Flour 488 (Thermo Fisher, A-11008) for 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... after 1h of secondary antibody incubation fluorochrome conjugated phalloidin (Alexa Fluor™ 546 Phalloidin, Invitrogen™) was added to the secondary antibody solution for F-actin membrane staining for another hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Each coverslip was then incubated for 1h at 37ºC with Duolink PLA probes (Thermo Fisher Scientific) diluted in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cut samples were incubated 1h at 37°C with Acridine orange 10 μM (A1301, Thermo Fisher), with or without Histamine 100 μM (H7127 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... only 4 μL of 1 mM FM 4-64 (Cat. number T13320, Thermo Fisher, Waltham, MA) was injected ...
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bioRxiv - Immunology 2019Quote: Stable cell lines were generated by co-transfection of pcDNA5/FRT/TO and pOG44 in a 9:1 ratio into HeLa FlpIN T-REx cells (kindly provided by the Hentze Lab, EMBL, Heidelberg) using Lipofectamine2000 (ThermoFisher Scientific) and selection with 10 µg/ml blasticidin and 500 µg/ml hygromycin B ...
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bioRxiv - Cell Biology 2022Quote: ... Liquid nitrogen was allowed to evaporate (30-60s) before 2mLs of extraction medium consisting of 75% (9:1 v/v) Methanol (ThermoFisher Scientific):Chloroform (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The amplified product was diluted with a ratio of 1 part cDNA to 9 parts 10mM Tris-HCl (Thermo Fisher, 15568025), and concentrations were measured with a dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 mM MEM sodium pyruvate) by pipetting with a fire-polished 9” borosiligate glass Pasteur pipet (Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2021Quote: ... to a final concentration of 9 mg.mL−1 that was measured by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham MA USA) with theoretical Mw = 39,390 g.mol−1 and ε280 = 50,037 mol−1.L.cm−1.
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bioRxiv - Biochemistry 2022Quote: ... Each dried aliquot of the non-polar layer was reconstituted in 50 μl of methanol: toluene (9:1, v/v) (Fisher Scientific) and transferred to 2 mL LC-MS amber vials with 300ul glass inserts ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were processed as previously described[9] and incubated in primary and secondary antibodies as follows: phalloidin-488 or phalloidin-633 1:100 (Thermo Fisher); αGNAT2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed in triplicates using 1 µl cDNA and 9 µl master mix consisting of 5 µl SYBR Green PCR Mix (4309155, Applied Biosystems), 3 µl H2O and 1 µl of forward and reverse primers (0.5 µM final concentration) ...
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bioRxiv - Pathology 2023Quote: ... 100% buffered Percoll was made by a 1:9 ratio of 10x PBS to Percoll (Fisher Scientific cat# 45-001-747).
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM MEM sodium pyruvate) by pipetting with a fire-polished 9” borosilicate glass Pasteur pipet (Fisher Scientific, Waltham, MA).
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bioRxiv - Genetics 2022Quote: ... SLITRK3-E606X-Myc or SLITRK3-C566R-Myc together with pCAGGS-IRES-GFP (SLITRK3:GFP=9:1)) were transfected into cultured hippocampal neurons at DIV 14-15 using Lipofectamine 3000 (ThermoFisher, L3000015). After 48 hours ...
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bioRxiv - Genetics 2022Quote: ... or SLITRK3-C566R-Myc together with pCAGGS-IRES-GFP (SLITRK3:GFP=9:1) were transfected into cultured hippocampal neurons at DIV 14-15 using Lipofectamine 3000 (ThermoFisher, L3000015). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... was purchased from Agilent under the product name Quantitative PCR Human Reference Total RNA). HeLa S3 and MCF7 RNAs used for initial FLEXI analysis (Figs. 1 to 9) were purchased from Thermo Fisher. All other cellular RNAs ...
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bioRxiv - Cell Biology 2024Quote: Pellets from the ultracentrifugation fractions 1 to 9 were resuspended in RIPA buffer with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific) with sonication in a Biorupter with settings 10x 30s on 30s off at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Parasites were cultured in HMI-9 medium supplemented with 10% inactivated FBS and 2.5 µg ml-1 G-418 (Life Technologies Europe) at 37°C in a humidified atmosphere containing 5% CO2 (G-418 was included for maintenance of TetR and T7RNAP) ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cells were transfected with 1:1 mEmerald-Zyxin-6 to mCherry-Paxillin-22 using Lipofectamine 3000 (ThermoFisher) per the manufacturer’s protocol and allowed to grow for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was replaced with a 1:1 mix of Essential 6 Medium (E6) (Thermo Fisher Scientific) and E8 medium ...