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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Human neuronal BE(2)-M17 cells (ATCC® CRL-2267) were maintained in a 1:1 ratio of F12 Hams and OptiMEM (GIBCO, USA) media supplemented with 10% horse serum (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with TBS for 10 min and then then nuclei were counterstained by incubation with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from a 1 mg/ml stock solution, Thermo Fisher Scientific #62248) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
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bioRxiv - Cell Biology 2023Quote: ... and the nucleus was stained with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from 1 mg/ml stock solution (Thermo Fisher Scientific #62248)) ...
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bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... cells with either MX2-T2A-TurboID or MX2-MUTT2A-TurboID lentivirus and selecting with 2 µg ml−1 puromycin (#ant-pr-1, Thermo Fisher Scientific) for 7 days ...
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bioRxiv - Genetics 2023Quote: ... The solution was then exchanged with primary antibody diluted in a staining solution (1% DMSO, 0.5% Triton X-100, and 2% NDS in PBS): Sox2 (1:200; Invitrogen, 14-9811-82) or AF647-conjugated β-catenin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... and fibroblasts were cultured for 2 days in DMEM/F12 (1/1) supplemented with 10 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France) and 1% Penicillin/Streptomycin (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: Neuroblastoma BE(2)-C) cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% Fetal Bovine Serum (Fisher Scientific FB12999102) and 1% Penicillin-Streptomycin (Lonza 17602E) ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Microbiology 2023Quote: ... mosquito bodies and legs were collected and placed in homogenizer tubes with a homogenizer bead and 500μl mosquito homogenization media (DMEM containing 2% FBS, 1% PenStrep, and 1% Fungizone (Gibco, Grand Island, NY)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ml of collagenase mix digestion solution (collagenase II + IV mixed at 1:1 ratio at 2 mg/ml -Thermo Fisher Scientific) was added to the tissue and incubated horizontally for 20 min at 37°C in a shaking incubator ...
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bioRxiv - Biochemistry 2024Quote: ... Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg) was injected onto a Dionex UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) with an additional RSLCnano pump run in online two-dimensional active modulation mode coupled to a Thermo Fusion Orbitrap mass spectrometer.
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bioRxiv - Bioengineering 2024Quote: ... NK cells and target cells were mixed at an effector to target (E/T) ratio of 4:1 (SK-BR-3 cells) or 2:1 (K562 cells) and Sytox Green (Thermo Fisher Scientific) was added at 100 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Then CB were diluted 1:1 (v/v) into a dilution buffer (1mM EDTA in PBS supplemented by 2% FBS (Thermo Fisher, #10099141)) and undelayed with density gradient medium LymphoPrep (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Star635P anti-guinea pig (Abberior, cat. 2-0112-007-1, 1:100 dilution) and Alexa Fluor 594 anti-rabbit (Thermo Fisher, cat. A-21207, 1:100 dilution), Alexa Fluor 488 anti-chicken (Thermo Fisher ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:1,000; Thermo Fisher, HYB-076-08-02), rabbit anti-CFP-10 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 1×106 HL-60 cells were seeded on cell 75 cm2 culture flask in 6 mL IMDM medium (Thermo Fisher Scientific, Waltham, MA, US) and incubated for 24 h in the presence of compound 1 at 10 nM and 10 μM concentrations ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µL deoxynucleotides (dNTPs) and 1 µL of RevertAid H Minus-MuLV reverse transcriptase (ThermoFisher Scientific) were added to a final volume of 20 µL ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated for 2 h with anti-rabbit IgG conjugated to Alexa-555 (1:200, Life Technologies) in PBT-BSA at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... fixed tissues were incubated for 2 hours with Alexa Fluor 546 Phalloidin (ThermoFisher Scientific 1:400) in PBS-0.1% Triton X-100 (PT) ...
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bioRxiv - Cell Biology 2020Quote: ... 1-2 µg of cDNA was diluted into 100 µl of Opti-MEM I Medium (Invitrogen) and mixed gently ...
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bioRxiv - Developmental Biology 2020Quote: ... Remaining of the plasmid was digested with 1 μl TURBO DNase (2 U/μl, ThermoFisher Scientific) for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 kPa polyacrylamide gels were made using 2 μL of blue fluorescent beads (200 nm; ThermoFisher), 18.8 μL of 40% acrylamide solution (cat no ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated 2 hours in a 1:250 dilution of TRITC-conjugated phalloidin (Molecular Probes, Eugene, OR), and subsequently imaged on a Zeiss LSM 700 confocal microscope.
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bioRxiv - Bioengineering 2019Quote: ... Each device was then washed 1× with PBS and 2× with Neuralbasal™-A medium (Invitrogen), with the last wash applied for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... then probed with a 1:2 000 dilution of primary α-Cas9 antibody (ThermoFisher 10C11-A12) in 5% skim milk-TBST overnight at 4°C ...
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Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... plants were germinated in 2 mL of media placed in 1-well microscopy slides (Thermo Fisher) and grown vertically ...
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bioRxiv - Bioengineering 2021Quote: ... and placed into a 2 mL Eppendorf tubes filled with 1 mL of RPMI 1640 (Gibco), 50 µM 2-mercaptoethanol (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... HBEC3-KT cells were incubated with 1 μM of 2’-7’-dichlorofluorescin diacetate (CM-H2DCFDA; Invitrogen) for 30 min at 37°C ...
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bioRxiv - Physiology 2021Quote: ... Adipogenic media for days 1-2 contained: DMEM with 4.5 g/L glucose (Thermo Fisher Scientific), 10% calf serum (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 40 mg tissue sample or 1×105 cultured cells were homogenized in 2 mL Trizol (Invitrogen), and then 1 mL chloroform was added ...
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bioRxiv - Cell Biology 2021Quote: ... 2% chick embryo extract (CEE,E.G.G. Technologies) and 1% (v/v) Penicillin/Streptomycin (P/S, Gibco), and 20 U/ml gamma interferon (γFN ...
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bioRxiv - Systems Biology 2021Quote: ... 1 µL of cells is spotted on a 2 percent low melting agarose pad (Invitrogen 16520050) made with 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... the spike-in culture was aliquoted in a 1:2 ratio with RNAlater™ solution (Invitrogen) on ice and stored at -80 °C.
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bioRxiv - Systems Biology 2020Quote: ... Non-transduced cells were eliminated by 72 h treatment with 2 μg mL−1 puromycin (Gibco).