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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Alexa488- conjugated antibody (1:40 dilution in 2%w/v milk blocking solution; Invitrogen, A11131) for one hour ...
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bioRxiv - Developmental Biology 2023Quote: Tadpoles were immersed in a solution containing 1 mM EdU (5-ethynyl-2’-deoxyuridine; Invitrogen) before fixation ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 1 ng/mL of fibroblast growth factor 2 (all from Thermo Fisher Scientific). The culture medium was renewed every two days ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were suspended in culture medium (Neurobasal medium, 2% B27, 1% GlutaMAX™, GIBCO, 1% antibiotic/antimycotic (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Genomics 2023Quote: ... Resuspended nuclei were incubated with 1-2 drops of NucBlue Live ReadyProbes Reagent (ThermoFisher Scientific) and immediately sorted using the DAPI channel on the Sony SH800S (Sony Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... in presence of Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, from Thermofisher, USA) and IL-2 (50 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... anti-claudin-2 (21H12) (Cat. No. 32-5600, 1:1000, Thermo Scientific, Waltham, MA, USA), anti-E-Cadherin (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody anti-rabbit Alexa Fluor 488 F(ab’)2 (A11070, Invitrogen, dilution 1:500) in PBS-Tx supplemented with 1% BSA was incubated overnight at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Caco-2 cell medium was also supplemented with 1% nonessential amino acids (Thermo Fisher Scientific). Cells were seeded with a density of 300.000 cells/mL DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... secondary antibodies (2°Abs included: Alexa- Fluor 647 donkey anti-rabbit [1:500, Thermo Scientific, A31573] ...
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bioRxiv - Biochemistry 2024Quote: ... 1× penicillin/streptomycin and 2 mM L-glutamine (GlutaMAX; each Thermo Fisher Scientific, VIC, Australia). Cells were grown in standard mammalian cell culture conditions at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... using a 2:1 v/v mixture of Freestyle293 media and Expi-293 media (ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 2 hours at room temperature in secondary antibodies (1:500, Invitrogen, AlexaFluors goat anti rabbit 546 Cat# A11035 and goat anti mouse 488 Cat# A11029 ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Western blots were performed under reducing conditions as previously detailed (6) to probe for PEMT (dilution 1:1000, Thermo Fisher Scientific, PA5-42383) and loading control GAPDH (dilution 1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... On day 6, the medium was changed to neural induction medium (NIM, DMEM/F12 GlutaMAX supplemented with 1:100 N2 supplement (Gibco™, 15502-048), 1 % Non-essential amino acids and 1 µg/ml Heparin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice by resuspending in 1 mL 1% bovine serum albumin (BSA; Bovagen, NZ) in PBS and centrifugation for 1 minute in a mySPIN™6 mini centrifuge (Thermo Scientific, USA). The resulting pellet was permeabilized for 10 min at room temperature in 0.1% Triton®X-100 (Sigma ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Neuroscience 2024Quote: ... and plated again in a dilution 1:6 in neural differentiation medium to induce neuroepithelial differentiation (NDM; 50% DMEM-F12 (Gibco, Grand Island, NY), 50% Neurobasal (Gibco ...
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Neuroscience 2023Quote: ... Each individual organoid was incubated in 1.5 mL of Trypsin/Accutase (1:1) containing 1 µL/mL of TURBO™ DNase (Thermo Fisher Scientific, AM2238, 2 U/µL) in a gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 2 washes of 2× SSC (Ambion AM9765) for 5min ...
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bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (2◻×◻10-5 M, ThermoFisher), penicillin (100◻IU◻per ml ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 0.05 mM 2-mercaptoethanol (2-ME; Gibco). Mouse melanoma cell line B16 (ATCC ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Microbiology 2020Quote: The plasmid SV-Psi--Env--MLV and L-LUC-SN were co-transfected with or without an envelope glycoprotein plasmid (pHCMV-VSV-G/pSARS-CoV-1/pSARS-CoV-2/pHIV-1 LAI gp160) into HEK293FT cells using Lipofectamine™ 3000 (ThermoFisher, US). Cell supernatants containing viruses were collected after 2 days of transfection ...
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bioRxiv - Genomics 2020Quote: ... or No Call (numerical representations 0, 1, 2, −1, respectively) using the BRLMM-P algorithm for 493,290 SNPs [25] (Affymetrix Power Tools (APT) Release 1.16.0) ...
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bioRxiv - Genomics 2020Quote: ... around 100 mg of liquid nitrogen frozen samples were ground with metal beads (2.5 mm diameter) in a Retschmill apparatus (24 hertz for 2×1 min) before re-suspending in 1 mL Trizol reagent (Thermo Fisher Scientific) and incubating at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Triton X-100 in 200 μL of PBS for 5 min and subsequently incubated in 100 μL of click reaction buffer (1× Click-iT cell reaction buffer [Thermo Fisher Scientific], 2 mM CuSO4, 1× Click-iT cell buffer additive [Thermo Fisher Scientific] ...
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bioRxiv - Biochemistry 2021Quote: ... equal numbers of THP-1 monocytes (2*105 ml−1) were seeded in RPMI 1640 without Lysine and Arginine (Thermo Fisher Scientific), supplemented either with natural amino acids (L-Lysine ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Developmental Biology 2021Quote: ... The colonies were lifted with a 1:1 Dispase/Collagenase enzyme mix (1mg/ml; Gibco; 17105-041 and 2 mg/ml; Gibco; 17104-019). and resuspended in 10 ml of Phase 1 medium (1:1 IMDM (Invitrogen ...