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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... 2 μl mouse anti-human CD14 APC (Invitrogen, MHCD1405), and 20 μg of human IgG were added to glass dishes containing PBMCs ...
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bioRxiv - Microbiology 2022Quote: ... and 50 IU/mL recombinant human IL-2 (Gibco). IL-2 was also supplemented to each culture for the maintenance of the cells.
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bioRxiv - Neuroscience 2023Quote: ... 2 ng/ml human Tgfb2 (ThermoFisher Scientific, 100-35B), 10 ng/ml murine Csf1 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: HEK293 cells were maintained at 37°C and 5% CO2 in phenol red-free Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Sigma-Aldrich (MilliporSigma)) ...
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bioRxiv - Genetics 2024Quote: ... Membrane was probed with anti-MBP antibody (Thermo Fisher MA527544). Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500 ...
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bioRxiv - Microbiology 2020Quote: ... incubated at 37°C (Cytomat 2, Thermo Scientific) with continuous shaking and OD595nm was measured every 30 min for 16 h in a Filtermax F5 multimode plate reader (Molecular Devices) ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa (ATCC CCL-2) and HEK293 (ATCC CRL-1573) cell lines were maintained in Dulbecco’s Modified Eagle Medium (Invitrogen 11965092) supplemented with 10% fetal bovine serum (Invitrogen 16140071) ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Biochemistry 2022Quote: HEK293S GnTI- cells were cultured in Yocon HEK293 medium (Yocon Biotechnology) supplemented with 1% FBS and 1% Antibiotic-Antimycotic (Gibco). HEK293F cells were cultured in SMM 293-TI medium (Sino Biological Inc) ...
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bioRxiv - Molecular Biology 2023Quote: ... and HEK293 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; TM4, HeLa: Gibco™ #41966 – high glucose, pyruvate; HEK293: Gibco™ #31966 – high glucose ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were transfected with Lipofectamine 2000 (Invitrogen) or Polyplus jetPRIME® reagent according to manufacturer protocol.
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bioRxiv - Genomics 2020Quote: Flp-In T-REx 293 (HEK293-derived; Invitrogen) and HEK293T (American Type Culture Collection ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were transfected with pcDNA6/TR (Invitrogen) to express the Tet repressor and selected with 10 μg/ml Blasticidin (Invivogen ...
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bioRxiv - Neuroscience 2019Quote: ... Transfection of HEK293 Flp-In cells (ThermoFisher Scientific) with the wildtype and mutant constructs was performed using lipofectamine 2000 according to the manufacturer’s recommendations (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were cultured using DMEM + GlutaMAX (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells and purified as described previously[26–28].
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in DMEM (GIBCO®) with 10% FBS (VWR® ...
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bioRxiv - Cell Biology 2022Quote: ... into HEK293 cells using Lipofectamine 3000 (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... into Flp-In Hek293 T-REx cells (Invitrogen) followed by selection with blasticidin (10 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were maintained by DMEM (Thermo Fisher) supplemented with 10% Fetal Bovine Serum (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown in DMEM (Thermo Fisher) media and transiently transfected with equal amounts of Cas9 and sgRNA expressing plasmids (PX459 ...
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bioRxiv - Microbiology 2021Quote: HEK293 cells were maintained in DMEM (Gibco, 41965039) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... FlpIn HEK293 cells were obtained from Thermo Fisher. Hras-/- ...
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bioRxiv - Systems Biology 2022Quote: The Flp-in T-REx HEK293 system (Invitrogen) was utilized for both stable plasmid transfection as well as doxycycline-induced expression ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC) were cultured in DMEM (Gibco) + 10% FBS (Seradigm) ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were grown in DMEM (Life Technologies) with 10% FBS at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were cultured in DMEM (Gibco, 11995065) + 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected using Lipofectamine 2000 (Invitrogen) and plasmid DNA purified with the Plasmid Plus Maxi Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected with Lipofectamine 3000 (Invitrogen) according to the protocol of the manufacturer and incubated for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were transfected with Lipofectamine200 (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: The HEK293 Flp-InTM cell line (Invitrogen, R78007) was purchased from the Duke Cell Culture Facility ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells were cultured in DMEM (Gibco-Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS Invitrogen-Thermo ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells were cultured in DMEM (Gibco-Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS Invitrogen-Thermo ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were cultured in DMEM (Life Technologies), supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in DMEM (Gibco) with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...