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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: The parB genes were recombined into a Gateway-compatible destination vector pET-His-MBP-TEV-DEST 56 via an LR recombination reaction (ThermoFisher). For LR recombination reactions ...
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bioRxiv - Biochemistry 2024Quote: Bacmids containing 6×His-MBP-TEV-nsp12 variants were generated using the Bac-to-Bac™ baculovirus expression system (Invitrogen). Sf9 cells were transfected with the bacmid DNA and baculovirus-containing cell supernatants were harvested 5 days after transfection ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% HI-FBS and 2mM EDTA (Invitrogen, 15575038).
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Microbiology 2021Quote: HEK293/TLR4 and HEK293/null cells (both Invitrogen) were maintained in 4.5 g/L glucose DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney293 (HEK293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta biologicals ...
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bioRxiv - Cell Biology 2021Quote: Human HEK293 suspension culture cells (Expi293F) were cultured in Expi293F expression media (Fisher Scientific) and maintained at 37°C in 8% CO2 in 125 mL spinner flasks on an orbital shaker rotating at 125 rpm ...
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2021Quote: ... MBP (Invitrogen PA1-10008), Synaptophysin (Synaptic Systems 101 203) ...
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bioRxiv - Microbiology 2021Quote: ... MBP-PfGet4 and MBP-PfGet2CD were affinity purified using amylose-resin (Thermo Fisher) and eluted with elution buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: Human embryonic kidney 293 (HEK293) cells were grown in high glucose DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... The vectors were then transfected into human embryonic kidney cells (HEK293) with Fectin-system (Invitrogen). Protein from culture supernatant was isolated as previously described [46 ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney fibroblasts (HEK293, RRID:CVCL_0045) were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 T-Rex (referred to as HEK293, cat.no. R71007, Invitrogen) and NIH3T3 cells were grown in DMEM Glutamax® (Thermo Fisher Scientific ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... HEK293S GnT1-/- or HEK293 cells (ThermoFisher Scientific, Cat. No. R78007) using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Neuroscience 2022Quote: ... Mbp (ThermoFisher, Mm01266402_m1 and Mm01266403_m1), Plp1 (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... chicken anti-MBP (Thermo Fisher; #PA1-10008 ...
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bioRxiv - Neuroscience 2023Quote: ... MBP (ThermoFisher PA-1-10008); CNP (Abcam ab6319(11-5b)) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 and HeLa cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) with 10% heat-inactivated FBS (BioInd) ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured under standard conditions (37°C, 5% CO2) in DMEM medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were maintained at 5% CO2 at 37°C in DMEM with glutamine (Invitrogen) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells 293 (HEK293) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: HMC3 human microglia or HEK293 cells were transfected with Lipofectamine 3000 with Plus reagent (Invitrogen L3000001) per manufacturer instructions with 0.8 μL of Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney 293 cells (HEK293, ECACC) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney (HEK293) cells from ATCC were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco #12430-054) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293, HEK293A) cell lines were grown in Dulbeco’s Modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... SAP97 or SAP102 fused to a C-terminal MBP were coated overnight at 4°C on ELISA plates (Nunc Maxisorb Immunoplates, #442404, Thermo) at a concentration of 100 ng per well ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...
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bioRxiv - Biochemistry 2022Quote: ... siRNA duplexes (siRNA B and C) were transfected into HEK293-PSMB2-HB cells using RNAiMAX (Invitrogen) dissolved in Opti-MEM (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... HEK293 cells were maintained at 37°C with 5% CO2 in DMEM media (ThermoFisher Cat #11965118) supplemented with 10% serum (Serum Plus II ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Gibco, cat 11965-092) + 10% FBS ...