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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) was used according to the manufacturer’s protocol with modest modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... caspase-3 and caspase-7 green apoptosis-assay reagent (Life Technologies) was added to the culture medium following manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... and CellEvent Caspase-3/7 Green ReadyProbes Reagent (ThermoFisher Scientific, R37111) (CellEvent ...
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bioRxiv - Immunology 2024Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher, C10423) was added to cultures at 20 μM and incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen; 1:1000) was used to measure apoptosis ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Cell Biology 2022Quote: ... 9 μl of carboxyl latex bead (4% w/v, 2 μm; Invitrogen, C37278), and 2 μl of Concanavalin A (1 mg/ml ...
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bioRxiv - Immunology 2024Quote: WT and Tlr2/4/9 -/- iBMDM were lysed in TRIzol reagent (Thermo Fisher) and RNA extraction was performed according to manufacturer protocol ...
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bioRxiv - Bioengineering 2020Quote: The IGFBP-derived NLS peptides (NLS-3 and NLS-5) were extracted from the bacteria using the B-Per 6xHis Fusion Protein Purification Kit (Thermo Scientific, USA), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... with 3 μL of Syto 9 and Propidium iodide (PI) dyes (ThermoFisher Scientific, USA) 1000-fold diluted in PBS (MilliporeSigma ...
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bioRxiv - Molecular Biology 2019Quote: ... a 3 µL aliquote was mixed with 9 µL NuPAGE LDS sample buffer (Invitrogen) and heated at 95 °C for 2 min to stop the reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was then further passivated by adding 9 μL of a freshly prepared aqueous solution of methyl-PEG4-NHS-Ester (10 mg/mL, Thermo Fisher, Cat. No. 22341), followed by adding 1 μL of 1 M NaHCO3 (pH 8.5) ...
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bioRxiv - Microbiology 2022Quote: ... One microliter of RNase H (5 U; Thermo Scientific) was subsequently added and the reaction mix was incubated at 37°C for 20 min.
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bioRxiv - Cell Biology 2023Quote: ... with 5% FBS (Gibco One Shot™; Fisher #A3160602) and Penicillin/Streptomycin (Mediatech ...
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bioRxiv - Bioengineering 2023Quote: ... one 5-minute wash with 95% ethanol (Fisher Scientific), one 5-minute wash with 70% ethanol and one 5-minute wash with 50% ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 7 dpf larvae were exposed to 5 mM BAPTA (Invitrogen) in E3 for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... 5 μM of 2′,7′-Dichlorofluorescin diacetate (DCFH-DA, Invitrogen) probe was added to each neutrophil subtype and incubated in the dark for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes adhered to glass coverslips in 12-well plates (1.5×106 cells/well) and were subsequently cultured for 7–9 days in RPMI (Gibco) with 10% FBS (Wisent ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: 5 × 106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... 5×106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10min before electroporation was performed as stated above.
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bioRxiv - Neuroscience 2023Quote: ... incubated at room temp in secondary antibodies (2h, streptavidin conjugated to Alexa Fluor 488 3:1000, ThermoFisher Scientific, Waltham, MA), and washed in PBS (overnight at 4°C) ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using OC43-nucleocapsid specific TaqMan primers and a probe 18 fluorescent- labelled with a 5’-FAM reporter dye and 3’-BHQ quencher (IDT) and AgPath-ID™ One-Step RT-PCR kit (AgPath AM1005, Applied Biosystems) on an ABI QuantStudio 3 platform (Thermo Fisher) ...
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bioRxiv - Immunology 2019Quote: ... 5’-CCCTACTGTATCCTCATG-3’/5’-CTTACCTCCTCTTCAATAGC-3’ PRKDC: 5’-GGGGCATTTCCGGGTCCGGG-3’/5’-TGCCCTGCCCCCCACTCTGC-3’ Amplicons were cloned using the Zero Blunt TOPO PCR Cloning kit (ThermoFisher), prepared as plasmids ...
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bioRxiv - Bioengineering 2021Quote: ... From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco, 17504044) was used and changed every 2-3 days.
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bioRxiv - Cancer Biology 2019Quote: ... then labelled with 1,1’-di-octa-decyl-3,3,3’,3’-tetra-methyl-indo-carbo-cya-nine perchlorate (DiI, ThermoFisher) and finally resuspended in a buffer containing 5% FBS in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... then disaggregated and labelled with 1,1’-di-octa-decyl-3,3,3’,3’-tetra-methyl-indo-carbo-cya-nine perchlorate (DiI, ThermoFisher) and finally resuspended in a buffer containing 5% FBS in PBS ...
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bioRxiv - Biophysics 2021Quote: We previously cloned the genes for TEM and CTX-M-9 β-lactamase into pET24-b plasmids (Life Technologies) for inducible protein expression under the T7 promoter.[21 ...
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bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mL B-27 (without Vit-A, Gibco, #.12587-010), 2.5 mL N-2 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Analysis of total 5-methyl-2ʹ-deoxycytidine (5-mdC) concentrations was performed using a Q exactive mass spectrometer (Thermo Fisher Scientific), equipped with an electrospray ionization source (H-ESI II Probe ...
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bioRxiv - Biochemistry 2021Quote: ... A DMSO solution with ≈4–7 mol pyrene-iodoacetamide (Molecular Probes) per mol of actin was added to the F-actin mix ...
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bioRxiv - Cell Biology 2024Quote: ... and CellTracker Blue (4-chloromethyl- 6.8- difluoro- 7- hydroxycoumarin; CMF2HC; Invitrogen), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... AMC (7-amino-4-methylcoumarin) fluorescence reference standard (ThermoFisher Scientific, A191): Prepare a 100 mM stock in DMSO ...
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bioRxiv - Microbiology 2019Quote: ... were maintained in HMI-9 (HMI-9 (Gibco), 20 % v/v FCS (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... CellEvent® Caspase-3/7 Green Detection Reagent (Thermal Fisher Scientific, C10423); CellTrace™ Violet (Thermo-Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... CellEvent™ Caspase-3/7 Green live staining detection reagent (Thermofisher Scientific) at 2 μM was prepared and added to the epithelial and vascular channels in order to visualize an apoptotic T-cell killing response ...
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bioRxiv - Cell Biology 2022Quote: ... CellEvent Caspase 3/7 Green Detection Reagent was obtained from Thermo Fisher Scientific and used according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2022Quote: ... HUVECs (passages 3-7) were washed with phosphate-buffered saline (PBS, ThermoFisher) and detached by trypsin/EDTA solution (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the QuantStudio 3 and 7 Real-Time PCR systems (Applied Biosystems).
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bioRxiv - Immunology 2023Quote: ... Apoptosis was assessed using the CellEvent Caspase-3/7 Detection Reagent (Invitrogen) over TCB-treatment duration or at specific intervals ...