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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for 6 cyclopropylpyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain punches as well as HBEC- 5i and bEnd.3 cells in 6-well plates using TRIzol (Invitrogen) homogenization and phase separation with chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Microbiology 2019Quote: ... 900µL of the phage prep was combined with 90µL sodium bicarbonate (1M, pH = 9.0) and 10µL (1mg/mL) amine reactive Alexa-633 probe (ThermoFisher, Waltham MA) and incubated at room temperature for 1 hour ...
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bioRxiv - Biophysics 2020Quote: Recombinant GST-tagged PH-domain of PLCd detecting the membrane lipid PI(4,5)P2 was produced and conjugated to of amine-reactive Alexa Fluor 647 carboxylic acid succinimidylester (Invitrogen) as previously described49 ...
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bioRxiv - Synthetic Biology 2022Quote: ... while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel, Belgium). Ethanol (96% vol. ...
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bioRxiv - Cancer Biology 2022Quote: ... Compensation beads (#01-2222-41) and ArC™ Amine Reactive Compensation Bead Kit (#10628) were purchased from Thermo Fisher. The cells were analyzed using a Cytek Aurora 5 Laser Spectral Flow Cytometer (Sony Biotechnology).
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bioRxiv - Neuroscience 2022Quote: ... Poly-D-ly-sine beads are prepared as follows: 30 million 4.5 µm aliphatic amine latex beads (Thermo Fisher) were incubated with poly-D-lysine (P7405 ...
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bioRxiv - Microbiology 2020Quote: ... Purified vesicles were covalently labelled with an amine-reactive Alexa Fluor 488 5-SDP ester dye (Molecular Probes/ThermoScientific).
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bioRxiv - Plant Biology 2019Quote: OefDef1.1 was labeled with DyLight 550 amine reactive dye following the protocol provided by the manufacturer (Thermo Fisher Scientific). Time-lapse confocal laser scanning microscopy was performed to monitor uptake and subcellular localization of the fluorescently labeled peptide as described previously (Islam et al. ...
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bioRxiv - Immunology 2021Quote: ... The spots on a High Capacity Amine Sensor chip (Bruker) were immobilized with streptavidin (ThermoFisher Scientific, Waltham, MA, USA) (50 µg/ml diluted in 10 mM sodium acetate buffer pH 5.0 ...
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bioRxiv - Synthetic Biology 2022Quote: The RBDs were biotinylated by amine coupling with sulfo-NHS-SS-Biotin according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... sharpened tungsten pins were mounted on a shaft and fluorescent dextran-amine (tetramethylrhodamine, 3,000 Da, Invitrogen, D3308; Alexa Fluor 488, 10,000 Da, Invitrogen, D22910) was recrystallized onto the tip of the pins (according to Glover ...
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: Quantum Dots QdotTM 655 ITKTM Carboxyl and QdotTM 655 ITKTM Amine (PEG) Quantum Dots (QDs) were procured from Invitrogen Corporation (Karlsruhe ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg of the oligonucleotide pool was labelled using the Alexa Fluor 488 Oligonucleotide Amine Labeling Kit (ThermoFisher Scientific) as per the kit instructions ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK)-293T cells in log-phase growth were rapidly transfected by using the standard Lipofect Amine reagent (Invitrogen), with GFP-Vector plus helper plasmids to develop virions (Cenciarelli et al. ...
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bioRxiv - Microbiology 2021Quote: Fluorescent polystyrene beads 0.2 μm in size (FluoSpheres sulfate microspheres F8848, carboxylate-modified microspheres F8811, amine-modified microsphere F8764; Thermo Fisher) were diluted 300 times to 0.02% (wt/vol ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μL of amine bearing microbeads solution was firstly reacted with 10 mM bis(sulfosuccinimidyl)suberate crosslinker (BS3; Life Technologies) in phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Neuroscience 2020Quote: ... Each probe is 18-22nt long with a 3’ end amine-modified nucleotide that allows directly couple to an NHS-ester dye according to the manufacturer’s instructions (Life Technologies). Dye-labeled probes were separated from the excess free dyes using the Qiagen Nucleotide Removal Columns ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...