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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Lipofectamine™ 3000 transfection reagent (InvitrogenTM, Cat# L3000001) and human PAR-1 si-RNA (CGGUCUGUUAUGUGUCUAUdTdT) were purchased from Invitrogen (USA) and Eurogentec (Belgium ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated overnight at 4ºC with mouse anti-human αSMA (Cat. 14-9760-82, Invitrogen: 1:500 dilution) and goat anti-human CD144 (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM L-glutamine at 37°C and in 5% CO2. Human RPE-1 cells (a gift from Dr. Hongtao Yu) were cultured in DMEM: F-12 medium (Invitrogen) supplemented with 10% FBS and 10mM L-glutamine at 37°C and in 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... chronic infected patient and HD groups by Luminex using the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (#EPX450-12171-901 ThermoFisher) according supplier’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Microbiology 2024Quote: ... with a plasmid encoding for a human furin ectodomain (residues 1–794) into 40 mLs of FreeStyle 293-F cells (ThermoFisher). Cells were transfected at a density of 1 million cells/mL using 25 kDa linear polyethylenimine (PEI) ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Developmental Biology 2021Quote: ... HA and Myc tagged proteins were visualized on an Amersham Hyperfilm MP (28906845, Cytiva) using SuperSignal WestDuo (34075, Thermo Fisher Scientific).
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were grown in 10 cm dishes and transfected with APEX2-tagged mammalian expression plasmids using Lipofectamine 3000 (Thermo Fisher). Cells were washed off the plate 12-15 hours post-transfection with Dulbecco’s PBS and then pelleted at 500x g ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa Kyoto cells were transfected with two guide RNA and one HDR donor plasmid (or 4 guide RNA and two HDR donor plasmids for the EGFP-STAG1 FLAG-mRFP-STAG2 double tagged cell line) using Lipofectamine 2000 (Invitrogen 11668019). Cells were grown for 7-10 days before sorting single cells into 96 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... RING1Bfl/fl conditional mESCs were transfected with pCAG vectors encoding 2xFlag-HA-tagged mouse wild-type or RING1B I53A/S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Genomics 2021Quote: ... MBP-tagged (pTHMT, Peti and Page, 2007) full-length CLAMP protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transiently co-transfected with 100 ng in total of an N- or C-terminal LgBiT and SmBiT tagged construct using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher). All possible combinations of the N-terminal and C-terminal-tagged split luciferase protein pairs were tested ...
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bioRxiv - Neuroscience 2019Quote: pLVX-EF1a-TS-EGFP-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged EGFP cDNA (DNA String byGeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissues were counterstained with wheat germ agglutinin (WGA) tagged with Alexa Fluor™ 647 (#A-31573, ThermoFisher Scientific, Waltham, MA) and DAPI (#H-1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed thrice with PBS and incubated with secondary antibody (donkey anti-goat IgG, tagged with Alexa Fluor 488, Thermo Fisher) at 1:500 dilution for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected with 100ng EGFP to mark cell bodies and 50-100ng of GFP tagged TDP-43 constructs using Lipofectamine 2000 (Invitrogen 52887), as previously described[41 ...
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bioRxiv - Biochemistry 2021Quote: Approximately 20 micrograms of purified 6xHis-tagged KRAS4BA59T were reduced with 5 mM tris(2-carboxyethyl)phosphine (ThermoFisher Scientific, Cat #77720) for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... RRV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using a sequence-tagged (indicated with lower case letters) RRV-specific RT primer (5′-ggcagtatcgtgaattcgatgcAACACTCCCGTCGACAACAGA-3′) with SuperScript IV reverse tran-scriptase (Life Technologies). RRV genomes were quantified by RT-qPCR using a tag sequence-specific reverse primer (5′-GGCAGTATCGTGAATTCGATGC-3′ ...
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bioRxiv - Biochemistry 2021Quote: ... Genes encoding pep86 (trademarked as ‘SmBiT’ (Dixon et al., 2015)) -tagged mitochondrial PCP proteins (from MGW Eurofins or Thermo Fisher Scientific) were cloned into either pBAD ...
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bioRxiv - Microbiology 2021Quote: Tagged diguanylate cyclases were generated by PCR amplification of each diguanylate cyclase followed by cloning into pENTR D-TOPO (ThermoFisher Scientific) and then transferred to either pRH016 (HA tag ...
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bioRxiv - Molecular Biology 2020Quote: ... Tagged amplicon pools were quantified using the Qubit™ 3.0 fluorometer and a Qubit™ dsDNA HS Assay Kit (Invitrogen, UK) and pooled with equimolar concentrations into a unique library ...
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bioRxiv - Molecular Biology 2020Quote: ... mESCs were transfected with 10ug pCAG vectors encoding 2xFlag-HA-tagged BAP1 wild-type or BAP1 C91S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were grown on coverslips overnight and transfected with an mGFP-tagged donor construct (GFP-SHANK3 SPN WT or GFP SHANK3 SPN R12E/K22D) and mCherry-tagged acceptor construct (mCherry-KRASG12V) using Lipofectamine® 3000 (Invitrogen) or jet PRIME (Polyplus ...
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bioRxiv - Cancer Biology 2022Quote: ... MIA PaCa-2 cells were grown on coverslips overnight and transfected with GFP-tagged SPN WT or SPN R12E/K22D using Lipofectamine® 3000 (Invitrogen) for 24 h as described above ...
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bioRxiv - Genomics 2022Quote: ... The human urothelial bladder tumor cells were transfected with the doxycycline-inducible GFP-tagged APOBEC3G vector and the PiggyBac transposase vectors with the Lipofectamine2000 transfection agent (ThermoFisher Scientific). G418 was used for the selection of the clones with stable genomic insertion of the GFP-tagged APOBEC3G component ...
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bioRxiv - Systems Biology 2022Quote: ... Individual wells were transfected with 25ng of plasmid expressing one tagged Parkin variant using a Lipofectamine 3000 kit (Invitrogen, L3000-015) via manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK 293T cells were transiently transfected with 5 µg of FLAG tagged full-length HRI construct and 5 µg of GFP tagged DELE1 constructs (DELE1CTD or mutants) using Lipofectamine 3000 transfection reagent (L3000008, ThermoFisher Scientific). GFP empty vector was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... H10,14,243F GPR65 or B2AR N-terminally tagged with FLAG were cultured in DMEM high glucose (Cytiva, SH3024301) supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079). Stable cell lines expressing one of the constructs were generated using Geneticin (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 5 min with 25 pM (single-label) or 5 pM (dual-label) biotin-tagged α-FLAG monoclonal antibody (ThermoFisher Scientific #MA1-91878-BTIN ...
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bioRxiv - Genetics 2023Quote: ... We used Gibson Assembly to add a 3x FLAG tag to the appropriate end and insert the tagged coding sequence into a pcDNA5/FRT vector (Thermo Fisher). We note that we overexpressed the propeptide of PSMB4 (removing amino acids 2-45).
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: Dextran-tagged Rhodamine-B (Rhod-Dex) with 10,000 Da and 70,000 Da molecular weights were from Invitrogen (Invitrogen 11466337 and 11590226). Mouse pre-osteoblast cells were cultured on 400 nm porous and non-porous surfaces in a 96-well plate 24 hours before the incubation with Rhod-Dex ...
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bioRxiv - Biochemistry 2024Quote: FL α-syn was covalently tagged at its lysine residues using NHS-Rhodamine (ex. 552 nm, em. 575 nm, Thermo Scientific). The probe was added to 100 µM α-syn in ∼ 8-fold molar excess ...