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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Synthetic Biology 2024Quote: ... His-tagged proteins were purified using 0.2 mL HisPur™ Ni-NTA Spin Columns from ThermoFisher according to the manufacturer’s protocol with the exception of carrying out 4 washing steps ...
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bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Genetics 2019Quote: ... chronic myelogenous leukemia cells and six independent transfections in SH-SY5Y (ATCC#CRL-2266) human neuroblastoma cells in two batches by lipofectamine3000 (Thermofisher Scientific cat. no L3000008). We then extracted DNA and RNA and performed 50 bp non-paired end sequencing reading from the 3’end ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Genomics 2021Quote: ... FACS sorted BST2+ T cells and BST2− T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, cat#11161D, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: MACS enriched CD4+ T cells and CD3+ T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, Cat# 11131D, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: siRNA probes targeting sfrp2 in human mesenchymal cells and frizzled-5 and -6 in AEC2 cells were obtained from Thermo Fisher (Supplemental Table 4). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific, Cat. #11161D) at a concentration of 25 μL/1 x 106 cells ...
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bioRxiv - Cell Biology 2020Quote: Normal human fibroblast cell line (AG08469, Coriell Institute for Medical Research) was maintained in Minimum Essential Medium (Gibco) and supplemented with 15% FBS (HyClone) ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Genomics 2021Quote: Human BLaER1 cells (14) were kindly provided by Thomas Graf (CRG, Barcelona) and grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was maintained in feeder-free culture on Geltrex matrix (A1413202 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human CD171 (i.e. antibody against neural cell adhesion molecule L1CAM; RRID: AB_2043813; Thermo Fisher Scientific, Waltham, MA) or anti-human tetraspanin CD81 (Ancell Corporation ...
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bioRxiv - Molecular Biology 2022Quote: ... Human HAP1 cells were obtained from Horizon Discovery and were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco), containing L-glutamine and 25 nM HEPES and supplemented with 10% Fetal Bovine Serum (FBS ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Molecular Biology 2021Quote: ... T cells were either frozen or stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Bioengineering 2019Quote: Human adult fibroblasts were harvested and re-suspended at 1.5×106 cells/mL in dermal proliferation media (ThermoFisher, DMEM-High Glucose supplemented with 1% Pen/Strep ...
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bioRxiv - Genetics 2021Quote: ... were co-cultured on irradiated murine embryonic fibroblasts in human embryonic stem cell medium [DMEM/F12 (ThermoFisher Scientific), 20% Knockout Serum Replacement (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney cells (293T; ATCC; ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), 10% fetal calf serum (FCS) ...
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bioRxiv - Biochemistry 2020Quote: CD3/CD28 Dynabeads were removed from stimulated primary human CD4+ T cells using a DynaMag-2 magnet (Invitrogen). For antibody staining ...
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bioRxiv - Bioengineering 2020Quote: The human fetal osteoblast (hOB) 1.19 cell line (ATCC) was expanded at 33.5°C in DMEM/F12 media (Gibco) containing 10% FBS (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: MMP1 in the secretome of cell lines was quantified with a MMP1 Human ELISA Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: DF-1 chicken fibroblasts and human HEK 293 or 293T cells were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Biologos) ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney 293T (Hek293T) cells were maintained in T75 flasks containing Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... mouse embryonic fibroblasts (MEFs) in human embryonic stem cell (hES) media composed of Knock-out Dulbecco’s modified Eagle’s medium (DMEM) (Gibco), 10% knock-out-serum replacement (Gibco) ...
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bioRxiv - Physiology 2020Quote: Human Embryonic Kidney 293 (HEK-293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2021Quote: Primary human T cells were thawed at Day 0 and activated with anti-CD3/CD28 Dynabeads (Thermo Fisher) at a 3:1 bead to T cell ratio and cultured in AIM V + 5 % heat-inactivated FBS ...
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bioRxiv - Bioengineering 2021Quote: ... MCF10A human mammary epithelial cells (ATCC) were cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media which consists of 2.50 mM L-Glutamine and 15 mM HEPES buffer ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: Human HaCaT epithelial keratinocytes and Vero cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: Human epithelial colon adenocarcinoma, Caco2, cells (ACC 169, DSMZ, Leipzig, Germany) were grown in DMEM/F-12 (Gibco, Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The human colon adenocarcinoma cell lines LS174T (derived from Caucasian colon adenocarcinoma) maintained in RPMI 1640 medium (Gibco) supplemented with 10% FBS (BI ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... the cells were incubated with primary antibodies such as rabbit anti-human ZO-1 IgG (Thermo Fisher Scientific) and mouse anti-human albumin IgG (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells (1.0 × 106) were transfected with human siRNA (10 nM) using Lipofectamine 3000 (Invitrogen, Waltham, MA). All siRNAs were ON-TARGETplus SMARTpool (Dharmacon ...
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney cells (293T; ATCC; ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2022Quote: Human A549 cells (ATCC CCL-185) and its derivatives were cultured in RPMI 1640 (Gibco catalog no. 11875) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8 and CD4 T cells were activated and expanded with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Human A549 type II alveolar epithelial cells (ATCC CL-185) were grown in Minimum Essential Medium (MEM, Gibco) supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS ...
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bioRxiv - Immunology 2022Quote: Human bladder carcinoma 5637 cells (ATCC, HTB-9) were grown in RPMI-1640 media (GIBCO, cat.no. 21875-034) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (1132D) were purchased from Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: Human induced pluripotent stem cells (iPS line WTSIi008-A, EBiSC, UK) were cultured in E8 medium (Life technologies) on Geltrex coating (Life technologies ...