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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Bioengineering 2024Quote: ... the sections were rinsed in PBS and the nuclei were counterstained and mounted with Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI; #S36939, Invitrogen). The slides were imaged at a 4X magnification using EVOS M7000 (ThermoFisher Scientific™ ...
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bioRxiv - Cancer Biology 2024Quote: ... and A-253 cells were serially diluted and seeded at three different dilutions (2, 4 and 128 cells/well) with 50 µL serum-free DMEM/F12 medium (Gibco) supplemented with 10 ng/ml human EGF (R & D systems) ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were plated on 25 mm coverslips coated with PLL (0.01%) and Collagen IV (4 µg/ml) and loaded with 10 µM Fura-2 AM (Thermo Fisher) with 0.02% Pluronic F-127 (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in 15-cm Petri dishes were grown to 60-70% confluency and then transfected with 4 µg of the UTR library of DNA templates (2 µg for each UTR library) using lipofectamine 3000 reagent (Invitrogen). Fourteen hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg of protein (or all of the sample for the MDF multiplexing experiment across 2 gels) was loaded into a NuPAGE 4∼12% Bis-Tris 1.5 mm gel (Invitrogen #NP0335) and gel electrophoresis performed ...
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bioRxiv - Microbiology 2024Quote: ... Transfectants were selected with either 4 nM WR99210 (Jacobus Pharmaceuticals; pSLI) or 2 μg/μl blasticidin S (Life Technologies; pSLI2). SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Developmental Biology 2019Quote: ... prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies, Carlsbad, CA; 1A12; 1:100). Following further buffer washes ...
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bioRxiv - Cancer Biology 2020Quote: ... new organoids were fixed with 4% paraformaldehyde for 1 day and then incubated with the phalloidin (1:40; Invitrogen) and Hoechst 33342 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Primary staining’s were performed overnight at 4 °C with antibodies to TRF2 and POT-1 (1:300; Abcam and ThermoFisher,); CD3 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... we loaded the cells with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Molecular Probes®) and incubated at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were stained with 2’,7’-Dichlorofluorescein diacetate (10 µM) (Thermo Fisher, USA) for one hour ...
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bioRxiv - Molecular Biology 2022Quote: ... then placed in 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (Invitrogen, Carlsbad, CA, USA) and incubated under vacuum for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-(and-6)-chloromethyl-2′,7′ dicholorodihydrofluorescein diacetate (CM-H2DCFDA; Molecular Probes C6827), was used to visualize ROS accumulation (excitation ...
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bioRxiv - Immunology 2022Quote: ... or 2.5μM of 5-(and-6)-Carboxy-2’,7’-Dichlorofluorescein Diacetate (DCFDA) (Invitrogen) was then added and incubated with the cells 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... follicles were treated with 10μM of 2′,7′ dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen- D399) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... or 5- (and -6)-chloromethyl- 2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA, ThermoFisher, C6827), or hydroxyphenyl fluorescein (HPF ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5A for 7 min using iBlotTM 2 dry Blotting Transfer system (Thermo Fisher). The membrane was blocked in Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Microbiology 2021Quote: ... BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene lipid stain was from Molecular Probes (Invitrogen/ Thermo Fisher Scientific).
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with primary antibodies (overnight, 4°C), washed (5X, PBS, 4°C) and incubated with species-specific secondary antibodies coupled to fluorophores (Thermo Fisher). Cells were then washed (5X ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were resolved for 4 hr at 100 V on 4% −8% polyacrylamide gradient Tris-acetate denaturing gels (Novex, Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were ultracentrifuged for 4 hours at 50.000 rpm at 4°C in a Sorvall MX 120/150 Plus MicroUltracentrifuge (Thermo Fisher Scientific) in S55A2 rotor ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized using isofluorane and perfused transcardially with 4 mL PBS followed by 4 mL RNAlater™ (Invitrogen; CAT # AM7020) using a peristaltic pump at a flow rate of 0.8mL/min ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted at blotting force 20 for 4 s at 4°C and 100% humidity and plunged into liquid ethane using a Vitrobot (Thermo Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... cell suspensions obtained as above were fixed in 4% paraformaldehyde and cytocentrifuged (Cytospin 4 Shandon, Thermo Scientific, 1200 rpm, 10 minutes). Slides of captured cells (n=3 patients for each group ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cellular response in each well was measured via fluo-4 intracellular calcium dye using the fluo-4 NW calcium assay kit (Molecular Probes) according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2019Quote: ... and CarboPac PA1 standard bore guard column (4 x 50 mm) plus CarboPac PA1 preparative IC column (4 x 250 mm, both Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: Protein samples in 1X LDS sample buffer containing 4% β-mercaptoethanol (BME) were separated by size on 4-12% Novex NuPage Bis-Tris SPS-PAGE (Invitrogen) gels (2 h ...
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bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
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bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, NW04122BOX) and transferred onto a PVDF membrane (Immobilon-FL ...
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bioRxiv - Neuroscience 2022Quote: ... Three successive 5-hour paraffin wax embedding stages were performed followed by cooling and sectioning of tissue on a Leica microtome into 4 × 4 μm thick serial sections that were collected on Superfrost (ThermoFisher Scientific) microscope slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Separation of anionic metabolites were performed using a Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific). The injection volume was 0.4 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...
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bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
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bioRxiv - Neuroscience 2024Quote: ... larvae and dissected adult brains were fixed overnight in paraformaldehyde (PFA; 4% in 1x phosphate-buffered saline) at 4°C then dehydrated overnight in 100% methanol (A4124, Fisher Scientific) at -20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Cell Biology 2023Quote: ... -4 blot force for 4 seconds and plunged frozen in liquid ethane using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected on a Titan Krios operating at 300 keV and equipped with a Gatan K3 direct detector and a 20 eV slit width energy filter ...