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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 4 mM Glutamine (Glutamax; Gibco Thermo Fisher Scientific), 2 mM Pyruvate and 200 μM Uridine (permissive complete medium ...
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bioRxiv - Molecular Biology 2024Quote: ... NuPAGE 4-12% Bis-Tris gels (Thermo Fisher) were loaded with protein samples and SDS-PAGE was conducted at a constant 180V for approximately 1hr ...
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bioRxiv - Physiology 2024Quote: ... or 4% paraformaldehyde (ThermoFisher Scientific, Waltham, MA, USA) for 2 hours at 4°C for labeling with anti-HuC/D and anti-IL-17R or anti-nestin ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... The lungs were inflated with 1-3 ml 2% UltraPure Low Melting Point Agarose (Invitrogen). Lungs and livers were fixed overnight at 4°C on a shaker ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% (v/v) Triton X-100) and analyzed by SDS-PAGE on 4-12% NuPAGE Bis-Tris gradient gels (Invitrogen) followed by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in Alexa488 Streptavidin for 2-4 hours in the same incubation solution at room temperature (S32354, Invitrogen). After final PBS washes ...
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bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Biochemistry 2022Quote: ... at a concentration of 2 x 105 cells per ml (4 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were desalted into reaction buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl and 4 mM Tris (2-Carboxyethyl) Phosphine (TCEP) (Invitrogen, T2556)) for removing DTT in storage buffer ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Biochemistry 2021Quote: ... Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher) at 4°C and 100% humidity ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were used at 1:200 dilution in PBT for 2 hours at room temperature or at 4 °C overnight (Goat anti-Chicken Alexa 488, ThermoFisher Scientific cat#A11039 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated with poly(I:C) at a final concentration of 2 μg/mL using 4 μL Lipofectamine 2000 (Thermo Fisher). At three hours post-transfection ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... The 4-micron Dionex IonPac AG-11-HC guard column (2 × 50 mm) and 4-micron IonPac AS11-HC analytical IC column (2.0 × 250 mm) were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Genetics 2022Quote: ... fixed ovaries and eggs were incubated in a 300 nM DAPI stain solution (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) on a shaking platform (150-200 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Immunology 2020Quote: ... CAR T cells were incubated for 30 min at 37°C with either 1μM fura-2 AM or fluo-4 AM (Life Technologies). T cells were then washed in HBSS and resuspended in TexMACS complete medium with 3% AB serum ...
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bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
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bioRxiv - Neuroscience 2020Quote: ... tissue was placed on slides and cover-slipped with mounting media containing DAPI (Fluoromount-G; 4’,6-diamidino-2-phenylindole; Invitrogen). All immunofluorescence runs contained tissue from more than one age (1 ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated for 30 minutes at 4°C with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher Scientific) while in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were plated in 10cm dishes at 80-90% confluency the day before transfection and transfected 16 hours later with 4 μg total DNA (2 μg of each plasmid) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The cells were placed onto 4 ml MSgg agar (1.5% w/v) set into each chamber of LabTek II 2-chamber microscope vessels (Thermo Scientific) set into various containers ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Cancer Biology 2019Quote: ... The FT tissue was cut into small pieces of around 2-4 mm in length and incubated in pre-warmed Digestive Medium containing Advanced DMEM/F12 (Gibco), 12mM HEPES (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were counter stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in ProLongTM Gold antifade mounting media (Invitrogen). Images were captured using Nikon C2 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in 2% BSA/PBS were incubated overnight at 4°C and secondary antibodies (Thermo Fisher Scientific, Alexa Fluor) were incubated for 2 hours at room temperature and washed with PBS-T ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Perkin-Elmer Life Sciences) for 15 min and then stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting in Prolong Diamond (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted onto glass slides with ProLong Gold Anti-Fade reagent with DAPI (4[prime],6-diamidino-2-phenylindole; ThermoFisher). Images were obtained using an Olympus BX60 inverted microscope equipped with phase and fluorescence optics ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...